Reactive Oxygen Species Signaling Differentially Controls Wound Healing and Regeneration

Huizen, Alanna V. Van; Hack, Samantha J.; Greene, Jacqueline M; Kinsey, Luke J.; Beane, Wendy S.

doi:10.1101/2022.04.05.487111

Cited by 5 publications

(2 citation statements)

References 76 publications

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“…Low and physiologically relevant levels of H 2 O 2 have been reported at <100 μM, 47 with 100 μM observed during inflammation 48 . Concentrations of as low as 500 μM can overwhelm the body's natural antioxidant mechanisms resulting in apoptosis in most systems 49 . NADFbs cultured in 3D within the luteolin gels resisted oxidative damage significantly better than controls.…”

Section: Discussionmentioning

confidence: 99%

Characterization and biological evaluation of a novel flavonoid‐collagen antioxidant hydrogel with cytoprotective properties

Nalband,

Sarker,

Khan

et al. 2023

J Biomed Mater Res

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Reactive oxygen species (ROS) play a critical and important role during wound healing but excess ROS at the wound site can lead to cellular damage and sub‐optimal healing. Minimizing oxidative damage to the wound site and any supplemental therapeutic cells can be achieved by delivering exogenous antioxidants. Collagen hydrogels are ideal wound care materials due to their biocompatibility, high water content, and porous, three‐dimensional architecture. Yet, they lack the inherent antioxidant activity that could help mitigate excess ROS at a wound site. This work formulates and evaluates the in vitro biocompatibility and antioxidant capabilities of collagen‐fibroblast hydrogels combined with the polyphenolic antioxidant luteolin. Collagen solutions mixed with luteolin readily assembled into robust hydrogels with increasing gel strength due to increasing concentrations of luteolin. SEM images confirmed a mean pore size of 2.2 μm and a drastically different macromolecular ultrastructure with extensive fine crosslinking relative to collagen. Adequate cell viability and metabolic activity of dermal fibroblasts cultured within the gels were measured across all formulations, resulting in higher antioxidant activity and more than double the protection to cells from oxidative damage than traditional collagen hydrogels. Given these results, luteolin‐collagen hydrogels demonstrate the potential for superior wound‐healing properties when compared to collagen alone.

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Section: Discussionmentioning

confidence: 99%

Characterization and biological evaluation of a novel flavonoid‐collagen antioxidant hydrogel with cytoprotective properties

Nalband,

Sarker,

Khan

et al. 2023

J Biomed Mater Res

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“…In general, ROS activate different signalling pathways involved in tissue damage, repair, and regeneration such as: Wnt/β‐catenin, fibroblast growth factors, vascular endothelial grow factors, Jun N‐terminal kinase and MAPK/ERK1/2, among others. ROS are able to promote the metabolic switch toward glycolysis, through HIF‐1α (Bandão et al., 2022; Van Huizen et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Homeostasis and evolution in relation to regeneration and repair

Cano‐Martínez,

Rubio‐Ruiz,

Guarner‐Lans

2024

The Journal of Physiology

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Homeostasis constitutes a key concept in physiology and refers to self‐regulating processes that maintain internal stability when adjusting to changing external conditions. It diminishes internal entropy constituting a driving force behind evolution. Natural selection might act on homeostatic regulatory mechanisms and control mechanisms including homeodynamics, allostasis, hormesis and homeorhesis, where different stable stationary states are reached. Regeneration is under homeostatic control through hormesis. Damage to tissues initiates a response to restore the impaired equilibrium caused by mild stress using cell proliferation, cell differentiation and cell death to recover structure and function. Repair is a homeorhetic change leading to a new stable stationary state with decreased functionality and fibrotic scarring without reconstruction of the 3‐D pattern. Mechanisms determining entrance of the tissue or organ to regeneration or repair include the balance between innate and adaptive immune cells in relation to cell plasticity and stromal stem cell responses, and redox balance. The regenerative and reparative capacities vary in different species, distinct tissues and organs, and at different stages of development including ageing. Many cell signals and pathways play crucial roles determining regeneration or repair by regulating protein synthesis, cellular growth, inflammation, proliferation, autophagy, lysosomal function, metabolism and metalloproteinase cell signalling. Attempts to favour the entrance of damaged tissues to regeneration in those with low proliferative rates have been made; however, there are evolutionary constraint mechanisms leading to poor proliferation of stem cells in unfavourable environments or tumour development. More research is required to better understand the regulatory processes of these mechanisms. image

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Regeneration ofLumbriculus variegatusrequires post-amputation production of reactive oxygen species

Beinart,

Gillen

2024

Preprint

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Animals vary in their ability to replace body parts lost to injury, a phenomenon known as restorative regeneration. Uncovering conserved signaling steps required for regeneration may aid regenerative medicine. Reactive oxygen species (ROS) are necessary for proper regeneration in species across a wide range of taxa, but it is unknown whether ROS are essential for annelid regeneration. Since annelids are a widely used and excellent model for regeneration, we sought to determine whether ROS play a role in the regeneration of the highly regenerative annelid,Lumbriculus variegatus. Using a ROS-sensitive fluorescent probe we observed ROS accumulation at the wound site within 15 minutes after amputation; this ROS burst lessened by 6 hours post amputation. Chemical inhibition of this ROS burst delayed regeneration, an impairment that was partially rescued with exogenous ROS. Our results suggest that similar to other animals, annelid regeneration depends upon ROS signaling, implying a phylogenetically ancient requirement for ROS in regeneration.

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Reactive Oxygen Species Signaling Differentially Controls Wound Healing and Regeneration

Cited by 5 publications

References 76 publications

Characterization and biological evaluation of a novel flavonoid‐collagen antioxidant hydrogel with cytoprotective properties

Characterization and biological evaluation of a novel flavonoid‐collagen antioxidant hydrogel with cytoprotective properties

Homeostasis and evolution in relation to regeneration and repair

Regeneration ofLumbriculus variegatusrequires post-amputation production of reactive oxygen species

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