Red cell and plasma cholinesterase activities in microsamples of human and animal blood determined simultaneously by a modified acetylthiocholine/DTNB procedure

Voß, G.; Sachsse, K.

doi:10.1016/0041-008x(70)90082-7

Cited by 194 publications

(47 citation statements)

References 12 publications

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“…Parameters were determined according to colorimetric enzymatic methods using reagent kits (Boehringer Mannheim GmbH DiagnosticaJ. Bloodacetylcholinesterase was determined by the method of Voss and Sachsse (1970). Haemoglobin, haematocrit, total and differential leukocytes count were also done.…”

Section: Discussionmentioning

confidence: 99%

Reference database of the main physiological parameters in Sprague-Dawley rats from 6 to 32 months

et al. 1998

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SummaryA reference database comprising body weight gain, exploratory activity, hot plate response, serum biochemistry, haematology, organ weight (%) and a complete anatomopathological study containing non-neoplastic and neoplastic lesions over 1200 Sprague-Dawley rats from 6 to 32 months is described. Comparisons between age and sex were analysed.Keywords Database; Sprague-Dawley rats, biological parameters Toxicology laboratories and other research institutions collect large bodies of reference data in order to support drug and chemical toxicity testing. However, many of these databases contain either a relatively small number of animals, a limited number of parameters, or poorly defined limitations by age, all of which limit their usefulness on a routine basis. In addition, it is often unclear whether the same test method was used throughout the data collection period. In our laboratory, we have set up and maintain a large reference database. The database covers not only the main physiological indicators, including results of the anatomicopathological studies, but also the major life span of this species. The data were classified into three main age groups, <6,6-18 and 18-32 months according to Wolford et a1. (1986), and sex. The present results correspond to data of special sentinel groups from different preclinical toxicological evaluations performed in our laboratory during the last 7 years.

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Section: Discussionmentioning

confidence: 99%

Reference database of the main physiological parameters in Sprague-Dawley rats from 6 to 32 months

et al. 1998

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“…The, whole brain AChE activity was measured by the method of Ellman, et al, (1961) and Voss and Sachsse, (1970). Change in absorbance per min of the sample was read spectrophotometrically at 420 nm.…”

Section: Estimation Of Brain Acetyl Cholinesterase (Ache) Activitymentioning

confidence: 99%

Effects of Erythropoietin on Memory Deficits and Brain Oxidative Stress in the Mouse Models of Dementia

Kumar

Jaggi

Singh

2010

Korean J Physiol Pharmacol

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The present study was undertaken to explore the potential of erythropoietin in memory deficits of mice. Memory impairment was produced by scopolamine (0.5 mg/kg, i.p.) and intracerebroventricular streptozotocin (i.c.v STZ, 3 mg/kg, 10 μl, 1 st and 3 rd day) in separate groups of animals. Morris watermaze test was employed to assess learning and memory. The levels of brain thio-barbituric acid reactive species (TBARS) and reduced glutathione (GSH) were estimated to assess degree of oxidative stress. Brain acetylcholinesterase enzyme (AChE) activity was also measured. Scopolamine/streptozotocin administration induced significant impairment of learning and memory in mice as indicated by marked decrease in Morris water-maze performance. Scopolamine/streptozotocin administration also produced a significant enhancement of brain AChE activity and brain oxidative stress (an increase in TBARS and a decrease in GSH) levels. Treatment of erythropoietin (500 and 1,000 IU/Kg i.p.) significantly reversed scopolamine-as well as streptozotocin-induced learning and memory deficits along with attenuation of those-induced rise in brain AChE activity and brain oxidative stress levels. It may be concluded that erythropoietin exerts a beneficial effect in memory deficits of mice possibly through its multiple actions including potential anti-oxidative effect.

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“…Brain cholinesterase activity was measured by the method of Ellman et al with a slight modification (Ellman et al, 1961;Voss and Sachsse, 1970). 0.5 ml of the cloudy supernatant liquid was pipetted out into 25 ml volumetric flask and dilution was made with a freshly prepared DTNB (5,5-dithiobis-2-nitrobenzoic acid) solution (10 mg DTNB in 100 ml of Sorenson phosphate buffer, pH 8.0).…”

Section: Estimation Of Brain Cholinesterasementioning

confidence: 99%

Pharmacological evidences for antiamnesic potentials of Phyllanthus amarus in mice

Joshi¹,

Parle²

2010

African Journal of Biomedical Research

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Red cell and plasma cholinesterase activities in microsamples of human and animal blood determined simultaneously by a modified acetylthiocholine/DTNB procedure

Cited by 194 publications

References 12 publications

Reference database of the main physiological parameters in Sprague-Dawley rats from 6 to 32 months

Reference database of the main physiological parameters in Sprague-Dawley rats from 6 to 32 months

Effects of Erythropoietin on Memory Deficits and Brain Oxidative Stress in the Mouse Models of Dementia

Pharmacological evidences for antiamnesic potentials of Phyllanthus amarus in mice

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