Reference database of the main physiological parameters in Sprague-Dawley rats from 6 to 32 months

Alemán, Celia; Más, Rosa; Rodeiro, Idania; Noa, Miríam; Hernández, Caridad; Menéndez, Roberto; Gámez, Rafael

doi:10.1258/002367798780599802

Cited by 84 publications

(52 citation statements)

References 4 publications

(5 reference statements)

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“…As expected, there was no body weight loss related to tumor induction or tumor development, either prior to treatment (tumor size <8 mm) or during treatment (using the placebo-treated groups as a reference). Rat growth followed a pattern similar to that reported in healthy female Sprague Dawley rats (28). No reduction in food intake was observed during the study.…”

Section: Discussionsupporting

confidence: 81%

DHA Effect on Chemotherapy-Induced Body Weight Loss: An Exploratory Study in a Rodent Model of Mammary Tumors

Hajjaji

Couet

Besson

et al. 2012

Nutrition and Cancer

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Body weight loss during the course of cancer disease has been associated with poor prognosis. Beside cancer-associated cachexia, weight loss can also result from chemotherapy. This work explored whether a model of mammary tumors in female Sprague Dawley rats could be appropriate to study the effect of doxorubicin on body weight, described weight change in this model, and assessed the effect of DHA on weight during chemotherapy. After tumor induction, rats were randomly assigned to a control or a DHA-enriched diet, and treated with doxorubicin or placebo twice a week for 2.5 wk (n = 6 in each group). Body weight, food intake, and tumor growth were monitored. Neither the induction of tumors nor their initial development impaired body weight gain. No reduction in food intake was observed. Tumor growth was similar between groups from day 1 to day 11. Although doxorubicin induced body weight loss from day 4 compared to placebo (P< 0.01) in rats fed the control diet, it did not induce body weight loss in rats fed the DHA-enriched diet (P = 0.02), indicating that DHA had a protective effect. These results indicate that doxorubicin can induce body weight loss in this model and that a DHA-enriched diet can prevent this effect.

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Section: Discussionsupporting

confidence: 81%

DHA Effect on Chemotherapy-Induced Body Weight Loss: An Exploratory Study in a Rodent Model of Mammary Tumors

Hajjaji

Couet

Besson

et al. 2012

Nutrition and Cancer

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“…Sprague Dawley (SD) rats are classified as young adult from the time of sexual maturity (P35) until 6 months, middle aged between 6 and 18 months, old (aged) after 18 months, and senile after 24 months with an average lifespan of 26-32 months (Alemán et al , 1998; Gao et al , 2011). Importantly, after 22-24 months, SD rats exhibit a 13% reduction in SN dopamine neurons (Gao et al , 2011).…”

Section: Resultsmentioning

confidence: 99%

Recombinant adenoassociated virus 2/5-mediated gene transfer is reduced in the aged rat midbrain

Polinski

Gombash

Manfredsson

et al. 2015

Neurobiology of Aging

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Clinical trials are examining the efficacy of viral vector-mediated gene delivery for treating Parkinson’s disease (PD). While viral vector strategies have been successful in preclinical studies, to date clinical trials have disappointed. This may be due to the fact that preclinical studies fail to account for aging. Aging is the single greatest risk factor for developing PD and age alters cellular processes utilized by viral vectors. We hypothesized that the aged brain would be relatively resistant to transduction when compared to the young adult. We examined recombinant adeno-associated virus 2/5 mediated green fluorescent protein (rAAV2/5 GFP) expression in the young adult and aged rat nigrostriatal system. GFP overexpression was produced in both age groups. However, following rAAV2/5 GFP injection to the substantia nigra (SN) aged rats displayed 40-60% less GFP protein in the striatum, regardless of rat strain or duration of expression. Further, aged rats exhibited 40% fewer cells expressing GFP and 4-fold less GFP mRNA. rAAV2/5-mediated gene transfer is compromised in the aged rat midbrain, with deficiencies in early steps of transduction leading to significantly less mRNA and protein expression.

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“…With regard to the recommended number of 10 rats per toxicological test in OECD Guideline 452 [20], the above recommendation of FDA should be applied to ensure that in average, more than 10 rats would be available for terminal testing. The reported survival rates of the control rats in Séralini et al [3] (70% for males, 80% for females) lie at or above the upper range presented in available literature on survival rates of SD rats in 2-year studies [28][29][30][31].…”

Section: The European Ramazzini Foundation For Oncologymentioning

confidence: 91%

Rat feeding studies with genetically modified maize - a comparative evaluation of applied methods and risk assessment standards

Meyer¹,

Hilbeck²

2013

Environ Sci Eur

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A 2-year rat feeding study with genetically modified NK603 maize sparked an international scientific and public debate as well as policy responses by the European Commission. The European Food Safety Authority (EFSA) evaluated the study as defective based on conceptual and methodological shortcomings by retroactive application of the recommendations of its recent guidance on 90-day feeding studies. Our comparative analysis of the three relevant NK603 publications, including a 90-day feeding study of Monsanto, showed that all of them satisfy or fail to satisfy the EFSA evaluation criteria to a comparable extent; the rejection of only one of the papers is, thus, not scientifically justified. We also show that EFSA's criteria are not standard practice in 21 other rat feeding studies lasting at a minimum of 12 months. The review reveals critical double standards in the evaluation of feeding studies submitted as proof of safety for regulatory approval to EFSA. We specifically argue that the current approach to declare statistically significant differences between genetically modified organisms and its parents as 'biologically irrelevant' based on additional reference controls lacks scientific rigor and legal justification in the European Union (EU) system. Only recently, the EU authorities started building up an implementing system based on its own legislation and supportive of the EU approach to risk assessment in the context of technology assessment. Until these issues are resolved, we do not expect that neither the public nor the scientific debate will subside.

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Reference database of the main physiological parameters in Sprague-Dawley rats from 6 to 32 months

Cited by 84 publications

References 4 publications

DHA Effect on Chemotherapy-Induced Body Weight Loss: An Exploratory Study in a Rodent Model of Mammary Tumors

DHA Effect on Chemotherapy-Induced Body Weight Loss: An Exploratory Study in a Rodent Model of Mammary Tumors

Recombinant adenoassociated virus 2/5-mediated gene transfer is reduced in the aged rat midbrain

Rat feeding studies with genetically modified maize - a comparative evaluation of applied methods and risk assessment standards

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