Red blood cell membrane fatty acid composition in infants fed formulas with different lipid profiles

Visentin, Silvana; Vicentin, Dimas; Magrini, Graciano; Santandreu, Fernanda; Disalvo, Liliana; Sala, Marisa; Fasano, María Victoria; González, Horacio F.

doi:10.1016/j.earlhumdev.2016.05.018

Cited by 8 publications

(3 citation statements)

References 27 publications

(32 reference statements)

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“…Breast milk FAs were extracted from the samples using modified Folch method (24,25). In brief, 500 µl of homogenized breast milk samples were added to a mixture of chloroform/methanol (v/v, 2:1) and 600 µl of normal saline and vortexed for 1 min.…”

Section: Milk Fatty Acid Analysismentioning

confidence: 99%

Comparisons of Breast Milk Fatty Acid Profiles in Overweight and Obese Women

et al. 2021

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Background and Objectives: Breast milk composition is affected by several factors such as maternal diet and body mass index. Milk fats include a major calorie content of the milk, which changes dramatically throughout the lactation. The aim of the present study was to assess breast milk fatty acid profile in overweight and obese mothers and its correlations with maternal body mass index.Materials and Methods: Milk samples were collected from 73 healthy lactating women referring to various health centers in Mashhad. Data were collected using food frequency questionnaire to assess the maternal dietary intakes. Breast milk fatty acid profile was assessed using gas chromatography.Results: Breast milk content of saturated fatty acids was higher in obese mothers than overweight mothers (p = 0.03) with positive correlations with maternal body mass index (r = 0.256; p = 0.037). Furthermore, breast milk monounsaturated fatty acids were negatively correlated with pre-pregnancy body mass index (r = -0.385; p = 0.003) and pre-pregnancy body weight (r = -0.276; p = 0.026). However, no significant correlations were observed between the maternal body fat mass and fat proportion with the breast milk fatty acids (p > 0.05). Moreover, no significant associations were seen between dietary saturated fatty acids, milk monounsaturated fatty acids, polyunsaturated fatty acids, n-3 polyunsaturated fatty acids and n-6 polyunsaturated fatty acids with the corresponding fatty acids in breast milk (p > 0.05). Conclusions:Based on the results, breast milk of the obese mothers included higher proportion of saturated fatty acids, while no significant differences were observed in the contents of milk monounsaturated fatty acids and polyunsaturated fatty acids between the overweight and obese women. Furthermore, maternal pre-pregnancy body mass index was negatively correlated with the contents of milk monounsaturated fatty acids, while it was positively correlated with saturated fatty acids contents.

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Section: Milk Fatty Acid Analysismentioning

confidence: 99%

Comparisons of Breast Milk Fatty Acid Profiles in Overweight and Obese Women

et al. 2021

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“…15,40 Se estudió su absorción e incorporación a la circulación en los niveles que alcanza la leche humana. 41,42 El recurso de LV diluida al medio con el agregado de 2 g de aceite y 5 g de azúcar por cada 100 mL debe considerarse como la última opción y hacer todos los esfuerzos para complementar las posibles deficiencias. Dos gramos de aceite de girasol, por ejemplo, aportarían aceite linoleico (54 %) y oleico (33 %), pero solo el 0,2 % de linolénico y no aporta DHA.…”

Section: áCidos Grasosunclassified

Riesgos nutricionales en lactantes que no reciben lactancia materna exclusiva en los primeros seis meses de la vida

2021

Arch Argent Pediat

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“…Recent studies have reported that milk formula-fed (MF) infants present more rapid weight gain during the first weeks of life compared to breastfed infants, and this appears to be associated with weight gain later in life [3][4][5]. The nutrient composition of human milk (HM) in comparison to milk formulas may play a significant role in the observed metabolic outcomes and the reported health differences when comparing these two neonatal diets [6][7][8][9][10][11]. Mitochondrial function and energy homeostasis impinge on all of these systems, but the role of infant diet and programming of cellular bioenergetics remains largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

Neonatal diet impacts liver mitochondrial bioenergetics in piglets fed formula or human milk

et al. 2020

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Background: Neonatal diet impacts many physiological systems and can modify risk for developing metabolic disease and obesity later in life. Less well studied is the effect of postnatal diet (e.g., comparing human milk (HM) or milk formula (MF) feeding) on mitochondrial bioenergetics. Such effects may be most profound in splanchnic tissues that would have early exposure to diet-associated or gut microbe-derived factors.Methods: To address this question, we measured ileal and liver mitochondrial bioenergetics phenotypes in male piglets fed with HM or MF from day 2 to day 21 age. Ileal and liver tissue were processed for mitochondrial respiration (substrate only [pyruvate, malate, glutamate], substrate + ADP, and proton "leak" post-oligomycin; measured by Oroboros methods), mitochondrial DNA (mtDNA) and metabolically-relevant gene expression analyses.Results: No differences between the diet groups were observed in mitochondrial bioenergetics indices in ileal tissue. In contrast, ADP-dependent liver Complex I-linked OXPHOS capacity and Complex I + II-linked OXPHOS capacity were significantly higher in MF animals relative to HM fed piglets. Interestingly, p53, Trap1, and Pparβ transcript abundances were higher in MF-fed relative to HM-fed piglets in the liver. Mitochondrial DNA copy numbers (normalized to nuclear DNA) were similar within-tissue regardless of postnatal diet, and were~2-3 times higher in liver vs. ileal tissue. Conclusion:While mechanisms remain to be identified, the data indicate that neonatal diet can significantly impact liver mitochondrial bioenergetics phenotypes, even in the absence of a change in mtDNA abundance. Since permeabilized liver mitochondrial respiration was increased in MF piglets only in the presence of ADP, it suggests that formula feeding led to a higher ATP turnover. Specific mechanisms and signals involved with neonatal dietassociated differences in liver bioenergetics remain to be elucidated.

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Red blood cell membrane fatty acid composition in infants fed formulas with different lipid profiles

Cited by 8 publications

References 27 publications

Comparisons of Breast Milk Fatty Acid Profiles in Overweight and Obese Women

Comparisons of Breast Milk Fatty Acid Profiles in Overweight and Obese Women

Riesgos nutricionales en lactantes que no reciben lactancia materna exclusiva en los primeros seis meses de la vida

Neonatal diet impacts liver mitochondrial bioenergetics in piglets fed formula or human milk

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