Recovery of sertoli cells by Allium cepa in Toxoplasma gondii infected rats

Abstract

Toxoplasma gondii is a protozoan parasite that is globally widespread and it affects men and animals. We investigated the effect of allium cepa (onion juice) on sperm parameters, testestrone level in male rats was experimentally infected by T. gondii. Wistar male rats (n=40) were allocated into four groups: control group (n=10), T 1 group that received tachyzoites of T. gondii (n=10), T 2 group that received tachyzoites of T. gondii plus fresh onion juice 1cc per rat daily by gavages method (n=10), and T3 group which received fresh onion juice 1cc per rat daily by gavage method (n=10). 30 days after inducing toxoplasma, 5cc blood were collected for measuring testosterone. Testes tissues of rats in all groups were removed; then, they were prepared for sertoli cells analysis. Serum total testosterones and sertoli were significantly decreased in groups that were infected with T. gondii, in comparison to control and onion groups. Moreover, comparing to control group (p<0.05), testes weights in toxoplasma group were drastically decreased. Since, in our study, T.gondii had grave effect on serum total testosterones, and because of applying fresh onion juice led to removing this harmful effect, it is suggested that eating of onion is useful in infected men.

Semen quality characteristics of Koekoek breeder cocks influenced by supplemental inclusion levels of onion and garlic mixture at 35-41 weeks of age

“…Khaki et al (2008) also demonstrated that the administration of onion juice (1 g/rat/day) for 20 days increases sperm count, viability, and motility in rats. The onion fresh juice can significantly increase the recovery of sperm health parameters such as count, motility, and serum total testosterone and total antioxidants capacity (Chakrabarti et al, 2003) levels in T. gondii-infected rats (Khaki et al, 2011). On the other hand, garlic has been used as medicine worldwide since ancient times (Arzanlou and Bohlooli, 2010).…”

“…On the other hand, garlic has been used as medicine worldwide since ancient times (Arzanlou and Bohlooli, 2010). It contains a wide variety of phytochemicals and micro components such as trace elements, vitamins, fructans, flavonoids, and sulphur compounds which can scavenge free radicals (Khaki et al, 2011). Therefore, importantly, one possible consequence of these ad libitum combinations of onion and garlic in the diet of Koekoek breeder cocks might be the pulling together of these qualities, which invariably influenced and improved the sperm health parameters of the birds as well as their semen viability, as observed in this study.…”

Effect of varying dietary supplemental inclusion levels of onion and garlic on semen quality characteristics of Hubbard white breeder broiler cocks aged 35-41 weeks old

“…Khaki et al (2008) also demonstrated that the administration of onion juice (1g/rat/day) for 20 days increases sperm count, viability and motility. The onion fresh juice can significantly increase the recovery of sperm health parameters such as count, motility and serum total testosterone and total antioxidants capacity (Chakrabatt et al, 2003) levels in T. gondii-infected rats (Khaki et al, 2011). On the other hand, garlic has been used as medicine worldwide since ancient times (Arzanlou et al, 2010).…”

“…On the other hand, garlic has been used as medicine worldwide since ancient times (Arzanlou et al, 2010). It contains a wide variety of phytochemicals and micro components such as trace elements, vitamins, fructans, flavonoids and sulphur compounds which can scavenge free radicals (Khaki et al, 2011). Therefore, importantly, one possible consequence of these ad-libitum combination of onion and garlic in the diet of Hubbard white breeder broiler cocks might be the pulling together of these qualities which invariably influenced and improved the bird's sperm health parameters as well as their semen viability as observed in this study.…”

Repairing Effect of Allium Cepa on Testis Degeneration Caused by Toxoplasma Gondii in The Rat

“…Studies on the effect of quercetin on oxidative damage in cultured chicken spermatogonial cells showed quercetin to have no deleterious effect on spermatogonial cells at doses of 1 mg/mL and 10 mg/mL. Khaki et al reported quercetin (1 mg/mL) increased the number of spermatogonial cells and decreased the mortality of Aroclor-induced oxidative damage (24,25). In this study, the effect of quercetin on serum MDA was determined, but the results indicated no obvious effect of quercetin on MDA production.…”