Recovery of pure B-phycoerythrin from the microalga Porphyridium cruentum

Řemínek, Roman; Álvarez‐Pez, José M.; Fernández, Francisco Gabriel Acién; Grima, E. Molina

doi:10.1016/s0168-1656(01)00385-6

Cited by 247 publications

(115 citation statements)

References 40 publications

Supporting

Mentioning

103

Contrasting

Unclassified

Order By: Relevance

“…Extraction with aqueous buffers has been used to obtain phycobiliproteins from P. cruentum (Bermejo Román et al, 2001 and lutein from C. vulgaris (Li et al, 2001). Extraction of many proteins requires biomass that has not been dried previously (Bermejo Román et al, 2002).…”

Section: Extraction and Purification Of Metabolitesmentioning

confidence: 99%

“…Some other chromatographic methods that have been used for recovering pure fatty acids (or equivalent esters) have included reverse phase chromatography, silica gel adsorption chromatography and argentated silica gel chromatography (Robles Medina et al, 1995;Belarbi et al, 2000). Proteins are usually purified using ion exchange chromatography (Bermejo Román et al, 2002).…”

Section: Extraction and Purification Of Metabolitesmentioning

confidence: 99%

See 1 more Smart Citation

Recovery of microalgal biomass and metabolites: process options and economics

Grima

Belarbi

Fernández

et al. 2003

Biotechnology Advances

1,850

692

View full text Add to dashboard Cite

Commercial production of intracellular microalgal metabolites requires the following: (1) largescale monoseptic production of the appropriate microalgal biomass; (2) recovery of the biomass from a relatively dilute broth; (3) extraction of the metabolite from the biomass; and (4) purification of the crude extract. This review examines the options available for recovery of the biomass and the intracellular metabolites from the biomass. Economics of monoseptic production of microalgae in photobioreactors and the downstream recovery of metabolites are discussed using eicosapentaenoic acid (EPA) recovery as a representative case study. D

show abstract

Section: Extraction and Purification Of Metabolitesmentioning

confidence: 99%

Section: Extraction and Purification Of Metabolitesmentioning

confidence: 99%

Recovery of microalgal biomass and metabolites: process options and economics

Grima

Belarbi

Fernández

et al. 2003

Biotechnology Advances

1,850

692

View full text Add to dashboard Cite

show abstract

“…Volume ratio (Vr): The volume ratio was given by the ratio between the volumes in the top and bottom phases (Bermejo et al 2002):…”

Section: Experimental Designsmentioning

confidence: 99%

Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

Antelo

Costa

Kalil

2015

Braz. arch. biol. technol.

View full text Add to dashboard Cite

C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w) PEG

show abstract

“…Depending on their absorption spectrum, PE can be divided into three main classes R-PE, B-PE, and C-PE [11]. It has been reported that PE is useful for development of fluorescent probes because of its high extinction coefficients, fluorescence quantum efficiency of more than 0.8, and stability as hexamers even at lower concentrations without showing fluorescence decrease [12e15].…”

Section: Introductionmentioning

confidence: 99%

“…B-PE is the main phycobiliprotein of Porphyridium species and it contains three subunits (ab) 6 g with molecular weights of 19.5 kDa (a and b subunits) and 29 kDa (g subunit) for a total molecular weight of 263 kDa [10]. This phycoerythrin normally has a double absorption peaks at~545 and 565 nm, and a shoulder peak at~498 nm, and a fluorescence emission maximum at 580 nm [11]. The price of B-PE products varies widely and is dependent on the purity ratio (A max /A 280 ).…”

Section: Introductionmentioning

confidence: 99%

One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum

Tang

Zhao

Bao

et al. 2016

Protein Expression and Purification

View full text Add to dashboard Cite

a b s t r a c t B-phycoerythrin (B-PE) was separated and purified from microalga Porphyridium cruentum using onestep chromatographic method. Phycobiliproteins in P. cruentum was extracted by osmotic shock and initially purified by ultrafiltration. Further purification was carried out with a SOURCE 15Q exchange column and analytical grade B-PE was obtained with a purity ratio (A 545 /A 280 ) of 5.1 and a yield of 68.5%. It showed a double absorption peaks at 545 nm and 565 nm and a shoulder peak at 498 nm, and displayed a fluorescence emission maximum at 580 nm. The analysis by sodium dodecyl sulfate epolyacrylamide gel electrophoresis (SDSePAGE) showed a bulky band between 18 and 20 kDa which could be assigned to subunits a and b and a low intensity band of 27 kDa assigned to g subunit. Our protocol provides attractive alternative to consider for the purification procedure to obtain analytical grade B-PE at commercial level.

show abstract

Recovery of pure B-phycoerythrin from the microalga Porphyridium cruentum

Cited by 247 publications

References 40 publications

Recovery of microalgal biomass and metabolites: process options and economics

Recovery of microalgal biomass and metabolites: process options and economics

Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum

Contact Info

Product

Resources

About