Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

Antelo, Francine; Costa, Jorge Alberto Vieira; Kalil, Susana Juliano

doi:10.1590/s1516-8913201502621

Cited by 16 publications

(8 citation statements)

References 29 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several techniques have been optimized to purify C-PC from the microalgae Spirulina (Antelo et al, 2015;Kamble et al, 2013;Kumar et al, 2014;Moraes and Kalil, 2009;Ramos et al, 2010), and from other microalgae (Benedetti et al, 2006;Abalde et al, 1998, Sørensen et al, 2013. However, a few works used membrane separation processes (Sørensen et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

C-Phycocyanin Purification: Multiple Processes for Different Applications

Figueira

Moraes

Kalil

2018

Braz. J. Chem. Eng.

Self Cite

View full text Add to dashboard Cite

C-phycocyanin is a potential nutraceutical/pharmaceutical candidate with functionalities that are better documented than those of health foods in general. Studies have demonstrated that C-phycocyanin has antioxidant and antitumor properties and potential activities against other diseases. However, its large-scale purification remains problematic and expensive. The aim of this work was to establish the best process for obtaining C-phycocyanin of different purities for different applications. The first step of this study was the maximization of the ultrafiltration process. Under the best ultrafiltration conditions, we evaluated the application of ultrafiltration, precipitation, and ion exchange chromatography (expanded and fixed beds) in different sequences and their effects on C-phycocyanin recovery and purification. It was possible to obtain C-phycocyanin that could be used as food dye with purity of 0.95 employing only diafiltration/ultrafiltration. With different process configurations, we obtained extracts that could be used as cosmetic dye with purity of 2.1 and biomarkers with purity of 3.0, and for therapeutic and biomedicine applications with an analytical grade (purity > 4.0).

show abstract

Section: Introductionmentioning

confidence: 99%

C-Phycocyanin Purification: Multiple Processes for Different Applications

Figueira

Moraes

Kalil

2018

Braz. J. Chem. Eng.

Self Cite

View full text Add to dashboard Cite

show abstract

“…At high concentration of PEG, there is an increase in system viscosity, reducing the homogenization, and, consequently, the phase separation is difficult (Pereira et al 2012). A similar behavior was observed by Antelo et al (2015), when they purified phycocyanin of A. platensis with ATPS composed of PEG 1500, 4000, 6000, and 8000 Da and potassium phosphate.…”

Section: Discussionmentioning

confidence: 54%

“…In this study, ATPSs composed by PEG and salt were used as protein purification strategy. This technique is reported as a good option in the purification of several proteins, especially enzymes or some specific group of protein, such as phycocyanin (Antelo et al 2015;Trentini et al 2020). Thus, the ATPS application was used as strategy to purify and recover A. platensis protein, previously extracted by ultrasonication and mechanical agitation (Lupatini et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Protein purification of Arthrospira platensis using aqueous two-phase system composed of polyethylene glycol and potassium phosphate/sodium citrate

et al. 2021

View full text Add to dashboard Cite

Aqueous two-phase systems (ATPS) stand out as an alternative technique for recovering and concentrating proteins. However, the study of ATPS to recover Arthrospira platensis protein is still poorly reported. This research used a sequential strategy of experimental design to evaluate the protein recovery from A. platensis protein extract using ATPS composed of polyethylene glycol (PEG: 1500, 4000, 6000, and 8000 Da) and saline solution (potassium phosphate and sodium citrate). The maximized conditions for A. platensis protein recovery were established by using 16% sodium citrate and 18% PEG 1500 Da, reaching 1.02 of purification factor and 75% protein recovery. Electrophoresis analysis showed protein bands between 5 and 15 kDa, characteristic size of phycobiliproteins. PEG and sodium citrate ATPS are a simple and low-cost alternative to the recovery A. platensis protein and show potential for industrial application.

show abstract

“…The purification of PC from Spirulina and other microalgae has been investigated and optimized by several authors [11][12][13][14][15][16]. Purification generally involves different techniques that include extraction, centrifugation and separation by chromatography or ion exchange and dialysis.…”

Section: Introductionmentioning

confidence: 99%

A Combination of Aqueous Extraction and Ultrafiltration for the Purification of Phycocyanin from Arthrospira maxima

et al. 2022

View full text Add to dashboard Cite

The purification of phycocyanin (PC) from Spirulina generally involves a combination of different techniques. Here, we report the results on PC yields from a combined aqueous extraction-ultrafiltration (UF) process of a strain of Arthrospira maxima cultivated in a farm devoted to producing PC with food-grade purity. Samples optimized from different biomass/solvent ratios were purified by using a polyethersulphone (PES) membrane with a molecular weight cut-off (MWCO) of 20 kDa. The UF system was operated at 2.0 ± 0.1 bar and at 24 ± 2 °C up to a volume concentration factor (VCF) of 5. A diafiltration (DF) process was conducted after UF in order to increase the PC recovery in the retentate. Samples were collected during both UF and DF processes in order to evaluate membrane productivity and PC purity. The average permeate fluxes of about 14.4 L/m2h were measured in the selected operating conditions and more than 96% of PC was rejected by the UF membrane independently ofthe extraction yields and times. The concentration of PC in the final retentate was 1.17 mg/mL; this confirmed the observed rejection and the final VCF of the process (about 5-fold when compared to the concentration of PC in the crude extract). In addition, the combination of UF and diafiltration allowed the removal of about 91.7% of the DNA from the crude extract, thereby improving the purity of the phycocyanin in the retentate fraction.

show abstract

Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

Cited by 16 publications

References 29 publications

C-Phycocyanin Purification: Multiple Processes for Different Applications

C-Phycocyanin Purification: Multiple Processes for Different Applications

Protein purification of Arthrospira platensis using aqueous two-phase system composed of polyethylene glycol and potassium phosphate/sodium citrate

A Combination of Aqueous Extraction and Ultrafiltration for the Purification of Phycocyanin from Arthrospira maxima

Contact Info

Product

Resources

About