Recovery of microorganisms from synovial and pleural fluids of animals using hyperosmolar media

16S rRNA Next Generation Sequencing Analysis Shows Bacteria in Alzheimer’s Post-Mortem Brain

“…However, the consistently high levels seen here in AD samples compared to normal brains and the apparent minimal contribution of post mortem interval along with the lack of significant contact with skin makes contamination an unlikely explanation for the P. acnes content of these data. Furthermore, the physiological characteristics of P. acnes (Buchanan et al, 1982; Bhatia et al, 2004; McDowell et al, 2013; Tanghetti, 2013), including its known ability to grow slowly in the cortex (Kornhuber, 1996), would make P. acnes a good candidate for a bacterial source of neuroinflammation in AD brains and the bacterial reads seen here would warrant further investigation.…”

“…Most commonly, it has been associated with post-operative lesions and implanted prostheses, but, also in chronic diseases such as lumbar region inflammation, endocarditis, sarcoidosis and in intracranial lesions (Buchanan et al, 1982; Bhatia et al, 2004; McDowell et al, 2013). Recently Branton et al (2016) have shown the presence of Proteobacteria and Actinobacteria (containing Propionibacteriaceae ) in both normal and multiple sclerosis affected brains; thus, normal brain has a microbiome consisting largely of Proteobacteria and Actinobacteria.…”