Atypical colony-like structures developing in control media and in clinical L-form cultures containing serum

Buchanan, Audria M.

doi:10.1016/0378-1135(82)90002-5

Cited by 7 publications

(1 citation statement)

References 18 publications

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“…The transferable L-form variant colonies observed in the present study resembled the nonreverting L-form variant colonies described by others (6, 13). They were identical to those detected in earlier work from this laboratory (10,11) in L-form cultures of synovial fluids from arthritic monkeys and dogs with steroid-responsive IPA and systemic lupus erythematosus. These colony-like bodies had to be ignored because of similarities with pseudocolonies of horse serum origin.…”

Section: Sods Are Cytoplasmic Scavengers Of O2;supporting

confidence: 87%

Nocardia asteroides recovery from a dog with steroid- and antibiotic-unresponsive idiopathic polyarthritis

Buchanan

Beaman

Pedersen³

et al. 1983

J Clin Microbiol

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This report describes a fatal case of idiopathic polyarthritis in a dog that was partially responsive to vigorous immunosuppressive treatment. Synovial fluids were cultured for L-forms at the following stages of disease: (i) acute arthritic relapse, (ii) incomplete remission, and (iii) death. No(a,(rdia asteroides UCD 1-581 was recovered from the L-form broth culture of the specimen taken during acute relapse, 5 weeks after inoculation, but not at any other stage of disease. Numerous conventional microbiological cultures were unproductive during all phases. Changes occurring in L-form plates included the formation of large irregular mineral deposits and many transferable bodies resembling pseudocolonies. Microscopic examination revealed the presence of many intracellular goldenbrown granules and acid-fast bodies in macrophages of the lung and bronchial lymph node tissues. The granules are believed to be the variants embedded in calcium deposits similar to those which developed in the L-form cultures in vitro. Fluorescence of these acid-fast bodies with antibody specific for superoxide dismutase of N. asteroides GUH-2 and labeled anti-immunoglobulin G established their relationship to the isolate. The unrelenting course of disease and the persistence of N. asteroides as an L-form in this animal despite vigorous immunosuppression suggest that this organism plays a direct role in the etiology of this disease.

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Section: Sods Are Cytoplasmic Scavengers Of O2;supporting

confidence: 87%