Recovery of lactoferrin from whey using cross-flow cation exchange mixed matrix membrane chromatography

Simultaneous clarification of Escherichia coli culture and purification of extracellularly produced penicillin G acylase using tangential flow filtration and anion-exchange membrane chromatography (TFF-AEMC)

“…Hence, the system required a minimum operating volume (∼100 mL in this study to cover the tubing volume and minimum working volume of the reservoir) for continuous operation. Such a minimum volume is not required for retentive separations, for which the product to be purified binds onto the membrane and the processing is typically operated in a recycling mode through which the permeate is recycled back to the reservoir without being drained from the process stream [1,18]. A major technical issue for the TFF-AEMC processing, particularly for that with a non-retentive separation mode, is that the increasing cell density of the retentate tends to aggravate the membrane fouling and consequently prevents the product harvest/purification.…”

Fabrication of polyimide hollow fiber membrane with well‐defined adsorption channels and binding sites for organics separation

“…Other benefits include reduced buffer usage due to a low void volume, lower pressure drops, lower compression, simple operational facility, and high scalability for industrial applications. In addition, column packing and column testing are eliminated when using membrane chromatography . The reported applications of membrane chromatography are mainly in bioseparation and purification (antibody and therapeutic protein production, purifying large biomolecules such as viruses and plasmid DNA) and the removal of heavy metal ions.…”

Solvent-resistant P84-based mixed matrix membrane adsorbers

“…The affinity of adsorptive solids for specific molecules can be classified in terms of hydrophobic, hydrophilic or charged functionalities, in particular ion exchange effects, molecular (imprinted) recognition or other specific interactions. Recently, mixed matrix adsorber membranes entered the field of solid phase extraction, offering the advantages of the membrane technology: easy scale-up, high throughputs and low pressure drop [18][19][20][21][22][23][24][25][26][27]. This adsorption based separation involves the preferential partitioning of the adsorbate molecules from the liquid phase onto the surface of substrate, here the adsorptive particles incorporated in the polymeric porous matrix, based on the various types of interactions between the biomolecules and particles.…”