Simultaneous clarification of Escherichia coli culture and purification of extracellularly produced penicillin G acylase using tangential flow filtration and anion-exchange membrane chromatography (TFF-AEMC)

Orr, Valerie; Scharer, Jeno M.; Moo‐Young, Murray; Honeyman, Charles H.; Fenner, Drew; Crossley, Lisa; Suen, Shing‐Yi; Chou, C. Perry

doi:10.1016/j.jchromb.2012.05.039

Cited by 13 publications

(9 citation statements)

References 21 publications

(23 reference statements)

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“…TFF devices tend to have large dead volumes, which are unequally distributed between the feed and permeate sides. But perhaps more importantly, the lateral resistances on the two sides of the membrane being unequal, problems identical to those with a radial-flow device, such as broad eluted peaks could be expected [28].…”

Section: Resultsmentioning

confidence: 99%

On the workings of laterally-fed membrane chromatography

Ghosh

Madadkar

2016

Journal of Membrane Science

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Section: Resultsmentioning

confidence: 99%

On the workings of laterally-fed membrane chromatography

Ghosh

Madadkar

2016

Journal of Membrane Science

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“…Membrane chromatography has been widely used for protein purification separation due to its good penetration, fast mass transfer, lower pressure drop, and easier to scale up, that is, gelatin pre‐concentration and enzymes extraction . One of the important filtration principles of membrane is the size exclusion, but the applications of traditional membrane for separating similarly sized proteins are still limited because the pore size of membrane is constant once prepared and the sieving/selectivity property of membrane is uncontrollable .…”

Section: Introductionmentioning

confidence: 99%

Similarly sized protein separation of charge‐selective ethylene‐vinyl alcohol copolymer membrane by grafting dimethylaminoethyl methacrylate

Huang

et al. 2018

J of Applied Polymer Sci

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Similarly sized protein separation was investigated using a charge‐selective membrane, which prepared by grafting dimethylaminoethyl methacrylate (DMAEMA) onto ethylene vinyl alcohol copolymer (EVAL) membrane. Bovine serum albumin (BSA) and bovine hemoglobin (BHb) was used as model proteins. P(DMAEMA), the weak cationic polyelectrolyte with ionizable tertiary amine groups, contributed to the charge‐selective separation for BSA and BHb. At pH 6.0, the grafted EVAL membrane surface was positively charged and BSA was negatively charged, while BHb was positively charged. The BSA was adsorbed onto the membrane surface due to electrostatic interaction and the BHb passed through the membrane into the permeate. The charge‐selective behavior resulted in the separation of the similarly sized protein. The maximum separation factors of static adsorption separation for model protein and binary mixture were 32.4 and 37.2, respectively. In the dynamic separation process, the maximum separation factor value was 6.2. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46374.

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“…In the area of recombinant technology, Orr et al. used the integration of tangential flow and membrane chromatography to obtain a one‐step purification of an extracellularly produced enzyme .…”

Section: Introductionmentioning

confidence: 99%

“…In food industry applications, it has been implemented for the recovery of bioactive proteins from industrial potato fruit juice [5,6] or for the separation of glycosylated caseinomacropeptide from skim milk whey [7]. In the area of recombinant technology, Orr et al used the integration of tangential flow and membrane chromatography to obtain a one-step purification of an extracellularly produced enzyme [8].…”

Section: Introductionmentioning

confidence: 99%

Considerations on the flow configuration of membrane chromatography devices for the purification of human basic fibroblast growth factor from crude lysates

Gieseler

Pepelanova

Meyer

et al. 2016

Engineering in Life Sciences

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Membrane chromatography possesses numerous advantages such as operation at high flow rates, low back pressure, ease of handling and scale up, which make the membrane adsorber process a viable alternative to conventional packed column chromatography. A purification process for the isolation of human recombinant basic fibroblast growth factor (FGF‐2) based on membrane chromatography was investigated using devices with different flow configurations. In the first process, the FGF‐2 capture step was performed with an axial flow device, while the alternative method achieved direct capture of FGF‐2 from unclarified cell lysate with a tangential flow device. In both processes, FGF‐2 purities exceeded 82% and the purified cytokine displayed high biological activity. Binding capacity (BC) from fermentation broth of the axial flow device was 28 mg/mL. This was 50% higher than the BC obtained with the tangential flow device under particle‐free supernatant conditions (18 mg/mL) and 150% higher compared to the BC achieved with unclarified cell lysate (11 mg/mL). While membrane chromatography in tangential flow mode omits clarification and thus reduces the number of stages in the downstream process, it displays lower peak resolution and leads to a lower overall process yield.

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Simultaneous clarification of Escherichia coli culture and purification of extracellularly produced penicillin G acylase using tangential flow filtration and anion-exchange membrane chromatography (TFF-AEMC)

Cited by 13 publications

References 21 publications

On the workings of laterally-fed membrane chromatography

On the workings of laterally-fed membrane chromatography

Similarly sized protein separation of charge‐selective ethylene‐vinyl alcohol copolymer membrane by grafting dimethylaminoethyl methacrylate

Considerations on the flow configuration of membrane chromatography devices for the purification of human basic fibroblast growth factor from crude lysates

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