Abstract:Similarly sized protein separation was investigated using a charge‐selective membrane, which prepared by grafting dimethylaminoethyl methacrylate (DMAEMA) onto ethylene vinyl alcohol copolymer (EVAL) membrane. Bovine serum albumin (BSA) and bovine hemoglobin (BHb) was used as model proteins. P(DMAEMA), the weak cationic polyelectrolyte with ionizable tertiary amine groups, contributed to the charge‐selective separation for BSA and BHb. At pH 6.0, the grafted EVAL membrane surface was positively charged and BSA… Show more
“…Corresponding to the water contact angle test results, in an acidic environment, the tertiary amine group at the end of the chain was protonated and had a positive charge, and the segments inside the aerogel could also stretch. The positive charge of the aerogel could produce electrostatic adsorption to negatively charged particles, so it could be used to adsorb BSA, lysozyme, methylene blue, etc. − More importantly, the adsorbed BSA could be released with an increase in pH.…”
Although chemical cross-linking could greatly improve the mechanical flexibility of nanocellulose aerogels, current cross-linking strategies still have some shortcomings, such as a complex cross-linking process or toxic cross-linking agents. Herein, a copolymer (PDMAEMA-co-PVTMS) containing polyorganosiloxane and a pH-responsive segment was designed and synthesized via free-radical polymerization. The polyorganosiloxane could covalently cross-link to a cellulose nanofiber (CNF) and the dimethylaminoethyl methacrylate (DMAEMA) section could endow the aerogel with pH-responsive properties. The prepared aerogel showed a three-dimensional (3D) porous structure with a specific surface area as high as 53.88 m 2 /g. Furthermore, the cross-linked aerogel had excellent mechanical flexibility and its maximum stress could be maintained above 71.3% of the initial value (11.88 kPa) after 50 cycles. More importantly, the aerogel could turn from a positive charge to a negative charge when the environment changed from acidity to alkalinity. It could be used to adsorb bovine serum albumin (BSA) in an acidic environment and adsorption capacity could reach 107 mg/g. It also could release 97% of adsorbed BSA in an alkaline environment. This work provided a new strategy to construct functional cellulose aerogels with excellent mechanical properties through structural design of a cross-linking agent containing organosiloxane.
“…Corresponding to the water contact angle test results, in an acidic environment, the tertiary amine group at the end of the chain was protonated and had a positive charge, and the segments inside the aerogel could also stretch. The positive charge of the aerogel could produce electrostatic adsorption to negatively charged particles, so it could be used to adsorb BSA, lysozyme, methylene blue, etc. − More importantly, the adsorbed BSA could be released with an increase in pH.…”
Although chemical cross-linking could greatly improve the mechanical flexibility of nanocellulose aerogels, current cross-linking strategies still have some shortcomings, such as a complex cross-linking process or toxic cross-linking agents. Herein, a copolymer (PDMAEMA-co-PVTMS) containing polyorganosiloxane and a pH-responsive segment was designed and synthesized via free-radical polymerization. The polyorganosiloxane could covalently cross-link to a cellulose nanofiber (CNF) and the dimethylaminoethyl methacrylate (DMAEMA) section could endow the aerogel with pH-responsive properties. The prepared aerogel showed a three-dimensional (3D) porous structure with a specific surface area as high as 53.88 m 2 /g. Furthermore, the cross-linked aerogel had excellent mechanical flexibility and its maximum stress could be maintained above 71.3% of the initial value (11.88 kPa) after 50 cycles. More importantly, the aerogel could turn from a positive charge to a negative charge when the environment changed from acidity to alkalinity. It could be used to adsorb bovine serum albumin (BSA) in an acidic environment and adsorption capacity could reach 107 mg/g. It also could release 97% of adsorbed BSA in an alkaline environment. This work provided a new strategy to construct functional cellulose aerogels with excellent mechanical properties through structural design of a cross-linking agent containing organosiloxane.
“…The EVAL membrane was prepared by phase inversion method as our previous work. [ 17 ] The EVAL membrane was immersed in 35 mL anhydrous THF and 0.28 mL TEA was added. Then, 0.14 mL 2‐BIB was gently added, and the solution was stirred at 35°C for 3 h. The pH‐responsive membrane was synthesized by grafting PDMAEMA via ATRP, using the EVAL‐Br membrane as the macroinitiator.…”
Section: Methodsmentioning
confidence: 99%
“…The measurement was conducted on the dead‐end filtration cell at a transmembrane pressure of 0.05 MPa. [ 17 ] The effective membrane area was 26.9 cm 2 and the water flux was calculated as follows:where J (L·m −2 ·h −1 ) is the flux; V (L) is the permeate volume; A (m 2 ) is the effective membrane area; t (h) is the filtration time. The phosphate buffer solutions with different pH values were used as the feed solutions.…”
Section: Methodsmentioning
confidence: 99%
“…The measurement was conducted on the dead-end filtration cell at a transmembrane pressure of 0.05 MPa. [17] The effective membrane area was 26.9 cm 2 and the water flux was calculated as follows:…”
Protein fractionation and purification remain challenging in membrane separation. Herein, a pH-responsive membrane was developed by grafting the brush-like poly(dimethylaminoethyl methacrylate) (PDMAEMA) via ATRP onto the ethylene vinyl alcohol copolymer (EVAL) membrane for protein fractionation. The PDMAEMA brushes are distributed in all pore channels of the membrane. Based on the electrostatic and sieving properties of the as-prepared pH-responsive membrane, three milk proteins are successfully separated from their mixture. The α-Lactalbumin (α-LA) preferentially permeates the membrane from the mixture at pH 3.2, and the bovine serum albumin (BSA) and lactoferrin (LF) can be separated at pH 6.4 based on the selective adsorption. This work proposes a promising candidate for protein separation by a pHresponsive membrane.
“…Furthermore, conventional ultrafiltration is limited to separating solutes with molecular size differences of more than 10 times [ 19 ]. The separation selectivity is hardly controllable for protein molecules of similar sizes [ 20 ].…”
Electrospun polyvinyl alcohol (PVA) nanofiber fabric was modified by Cibacron Blue F3GA (CB) to enhance the affinity of the fabric. Batch experiments were performed to study the nanofiber fabric’s bovine hemoglobin (BHb) adsorption capacity at different protein concentrations before and after modification. The maximum BHb adsorption capacity of the modified nanofiber fabric was 686 mg/g, which was much larger than the 58 mg/g of the original fabric. After that, the effect of feed concentration and permeation rate on the dynamic adsorption behaviors for BHb of the nanofiber fabric was investigated. The pH impact on BHb and bovine serum albumin (BSA) adsorption was examined by static adsorption experiments of single protein solutions. The selective separation experiments of the BHb–BSA binary solution were carried out at the optimal pH value, and a high selectivity factor of 5.45 for BHb was achieved. Finally, the reusability of the nanofiber fabric was examined using three adsorption–elution cycle tests. This research demonstrated the potential of the CB-modified PVA nanofiber fabric in protein adsorption and selective separation.
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