Selective Adsorption and Separation of Proteins by Ligand-Modified Nanofiber Fabric

Abstract

Electrospun polyvinyl alcohol (PVA) nanofiber fabric was modified by Cibacron Blue F3GA (CB) to enhance the affinity of the fabric. Batch experiments were performed to study the nanofiber fabric’s bovine hemoglobin (BHb) adsorption capacity at different protein concentrations before and after modification. The maximum BHb adsorption capacity of the modified nanofiber fabric was 686 mg/g, which was much larger than the 58 mg/g of the original fabric. After that, the effect of feed concentration and permeation rate on the dynamic adsorption behaviors for BHb of the nanofiber fabric was investigated. The pH impact on BHb and bovine serum albumin (BSA) adsorption was examined by static adsorption experiments of single protein solutions. The selective separation experiments of the BHb–BSA binary solution were carried out at the optimal pH value, and a high selectivity factor of 5.45 for BHb was achieved. Finally, the reusability of the nanofiber fabric was examined using three adsorption–elution cycle tests. This research demonstrated the potential of the CB-modified PVA nanofiber fabric in protein adsorption and selective separation.

Application of nanofibrous protein for the purification of contaminated water as a next generational sorption technology: a review

“…The efficiencies of the membrane were due to the possession of multiple sites for attachment and interaction, considerable low mass transfer limitation, convoluted porous structure, evenly dispersed and aggregated surface morphology, and large surface area-to-volume ratios (Liu and Mukai 2021). Other potentials include concurrent separation of two or more chemicals with similar and different structures, the reusability of the nanofibrous membranes, reduction in the multi-step chemical reactions leading to minimal damage to the nanofibers' shape and performance, improvement in the membrane's operational flexibility and thereby reducing both material and labor costs (Liu and Mukai 2021). The sorption technology has created the opportunity for the scaling-up of the process thereby enhancing the strength and toughness in terms of improving the membranes' mechanical stability.…”

“…These properties include excellent mechanical dynamics, robust biocompatibility, and barrier resistivity, though with limited hydrophilicity in practical applications. These materials could be functionally modified to enhance their compatibility with other biomass materials thereby improving the level of hydrophilicity in large-scale industrial applications (Liu and Mukai 2021). Additionally, the modification is mainly aimed at improving the hydrophobic property, solubility, and compatibility of the membrane in the formation of the desired composite substrate.…”

Preparation of the Carbonized Zif−8@PAN Nanofiber Membrane for Cadmium Ion Adsorption

“…Nanofiber membranes produced by the electrospinning technique are of high specific surface area and porosity due to their low diameter of several tens of nanometers to few hundred nanometers. As a result, they have been widely used in the field of adsorption and separation, air filtration, drug delivery system, tissue engineering, antimicrobial applications, catalysis, energy storage devices, and controllable water absorption [12][13][14][15][16][17][18][19]. Polyacrylonitrile (PAN) is a kind of polymer material with abundant functional cyano−groups (-CN) on its macromolecular chain, which results in its excellent thermal and chemical stability, and resistance to solvent, oxidative degradation, sunlight, and weather.…”

Electrospun Composite Nanofibers for Functional Applications

“…Selective separation experiments were carried out at the optimal pH value. A reusability test was carried out by performing three adsorption–elution cycles [ 33 ]. Task-specific functionalized polymeric nanofiber mats were fabricated from homocysteine thiolactone and polyvinylpyrrolidone through electrospinning.…”