Reconociendo el virus del chikunguña

Calvo, Eliana; Archila, Edwin Darío; López, Lady S.; Castellanos, Jaime E.

doi:10.7705/biomedica.5797

Cited by 6 publications

(3 citation statements)

References 106 publications

(126 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Post-translational modifications of E-related proteins occur in the endoplasmic reticulum, Golgi complex, and plasma membrane. In the cytoplasm, capsid proteins interact with the vgRNA to package it, and this complex moves to the cell membrane in the presence of E proteins to assemble and release viral particles (Velandia and Castellanos, 2011 ; Calvo et al, 2021 ).…”

Section: Mosquitoes and Arbovirusmentioning

confidence: 99%

“…Furthermore, Wolbachia causes endoplasmic reticulum stress, disrupting viral envelope protein processing. The bacteria also alters microtubule organization ( Figure 2A -VIII) and vesicular trafficking (Ferree et al, 2005 ), a key factor in arbovirus entry (Velandia and Castellanos, 2011 ; Calvo et al, 2021 ), and Wolbachia wRNase HI degrades DENV RNA, suppressing early infection (Hussain et al, 2023b ), Wolbachia could also affects EVs biogenesis and their involvement in arbovirus infection.…”

Section: Mosquitoes and Arbovirusmentioning

confidence: 99%

See 1 more Smart Citation

A tangled threesome: understanding arbovirus infection in Aedes spp. and the effect of the mosquito microbiota

Mantilla-Granados,

Castellanos,

Velandia-Romero

2024

Front. Microbiol.

View full text Add to dashboard Cite

Arboviral infections transmitted by Aedes spp. mosquitoes are a major threat to human health, particularly in tropical regions but are expanding to temperate regions. The ability of Aedes aegypti and Aedes albopictus to transmit multiple arboviruses involves a complex relationship between mosquitoes and the virus, with recent discoveries shedding light on it. Furthermore, this relationship is not solely between mosquitoes and arboviruses, but also involves the mosquito microbiome. Here, we aimed to construct a comprehensive review of the latest information about the arbovirus infection process in A. aegypti and A. albopictus, the source of mosquito microbiota, and its interaction with the arbovirus infection process, in terms of its implications for vectorial competence. First, we summarized studies showing a new mechanism for arbovirus infection at the cellular level, recently described innate immunological pathways, and the mechanism of adaptive response in mosquitoes. Second, we addressed the general sources of the Aedes mosquito microbiota (bacteria, fungi, and viruses) during their life cycle, and the geographical reports of the most common microbiota in adults mosquitoes. How the microbiota interacts directly or indirectly with arbovirus transmission, thereby modifying vectorial competence. We highlight the complexity of this tripartite relationship, influenced by intrinsic and extrinsic conditions at different geographical scales, with many gaps to fill and promising directions for developing strategies to control arbovirus transmission and to gain a better understanding of vectorial competence. The interactions between mosquitoes, arboviruses and their associated microbiota are yet to be investigated in depth.

show abstract

Section: Mosquitoes and Arbovirusmentioning

confidence: 99%

Section: Mosquitoes and Arbovirusmentioning

confidence: 99%

A tangled threesome: understanding arbovirus infection in Aedes spp. and the effect of the mosquito microbiota

Mantilla-Granados,

Castellanos,

Velandia-Romero

2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…While rare, CHIKV seems to be capable of infecting the lungs, causing acute respiratory distress syndrome (ARDS) and some cases of respiratory failure, which have resulted in the death of at least one patient [51,[81][82][83][84][85][86][87][88]. In addition to human patients, mouse models of CHIKV infection have also exhibited inflammatory cells in the alveolar sacs at the end of bronchioles in the lungs [74].…”

Section: Rare Respiratory Complicationsmentioning

confidence: 99%

Chikungunya Immunopathology as It Presents in Different Organ Systems

Traverse

Millsapps

Underwood

et al. 2022

Viruses

View full text Add to dashboard Cite

Chikungunya virus (CHIKV) is currently an urgent public health problem as high morbidity from the virus leaves populations with negative physical, social, and economic impacts. CHIKV has the potential to affect every organ of an individual, leaving patients with lifelong impairments which negatively affect their quality of life. In this review, we show the importance of CHIKV in research and public health by demonstrating the immunopathology of CHIKV as it presents in different organ systems. Papers used in this review were found on PubMed, using “chikungunya and [relevant organ system]”. There is a significant inflammatory response during CHIKV infection which affects several organ systems, such as the brain, heart, lungs, kidneys, skin, and joints, and the immune response to CHIKV in each organ system is unique. Whilst there is clinical evidence to suggest that serious complications can occur, there is ultimately a lack of understanding of how CHIKV can affect different organ systems. It is important for clinicians to understand the risks to their patients.

show abstract

Molecular and biological characterization of an Asian-American isolate of Chikungunya virus

et al. 2022

View full text Add to dashboard Cite

Chikungunya virus is an arthropod-transmitted virus that causes chikungunya fever, a disease characterized by severe muscle and joint pain. In 2013, the virus was introduced to the Americas and caused approximately 2.7 million cases of infection during the subsequent two years. The lack of knowledge regarding the biological behavior of the viral strains circulating during the outbreak motivated the characterization of an isolate from the Colombian outbreak, starting from analysis of the complete genome to the biological behavior in vitro. The full genome was retrieved using next-generation sequencing. The infective and replicative capacities were evaluated in HEK293T, Huh-7, and MRC-5 cell lines. The infection rates were determined by flow cytometry, and the cytopathic effect was assessed by a resazurin fluorescent metabolic assay. The viral yield was quantified using the virus plaque formation assay, while the viral proteins and genomic RNA kinetics were subsequently evaluated by western-blot and RT-qPCR. The COL7624 isolate clustered with other American and Caribbean sequences in the Asian American lineage. The T669A substitution in E2 protein distinguished it from other Colombian sequences reported in 2014. After 48 h post infection (hpi), the three cell lines analyzed reached infection percentages exceeding 65%, generating a high load of infectious viral progeny. The infection kinetics indicated that the replication peak of this CHIKV isolate is around 24 hpi, although gRNA is detectable in the culture supernatant from 4 hpi onwards. The infection caused the overexpression of interferon and pro-inflammatory cytokines, such as IL-1β, TNF-α, and IL-8. The COL7624 CHIKV isolate exhibited a high infective and replicative capacity as well as activation of cellular immune responses, similar to isolates belonging to the other genotypes.

show abstract

Reconociendo el virus del chikunguña

Cited by 6 publications

References 106 publications

A tangled threesome: understanding arbovirus infection in Aedes spp. and the effect of the mosquito microbiota

A tangled threesome: understanding arbovirus infection in Aedes spp. and the effect of the mosquito microbiota

Chikungunya Immunopathology as It Presents in Different Organ Systems

Molecular and biological characterization of an Asian-American isolate of Chikungunya virus

Contact Info

Product

Resources

About