Recombinase polymerase amplification-lateral flow (RPA-LF) assay combined with immunomagnetic separation for rapid visual detection of Vibrio parahaemolyticus in raw oysters

Point-of-Need Diagnostics for Foodborne Pathogen Screening

“…The Wang lab further explored sensitivity improvements by using IMS to capture and isolate Vibrio parahaemolyticus in contaminated raw oyster samples. 109 IMS decreased the LOD to 2 CFU/g after 4 h of enrichment from 2 × 10 6 CFU/g in the oyster samples. However, the increased sensitivity of RPA-LFA compromises assay time; though it exhibits lower time to detection than traditional methods like PCR, this hybrid technique is much less rapid than RPA or LFA alone.…”

Evaluation of loop-mediated isothermal amplification assay along with conventional and real-time PCR assay for sensitive detection of pathogenic Vibrio parahaemolyticus from seafood sample without enrichment

“…To date, all the LAMP-based assays developed for the detection of pathogenic V. parahaemolyticus targeting trh and tdh was not validated by direct seafood samples, and the studies involved either enrichment or use of pure cultures. The other available isothermal amplification methods for the detection of V. parahaemolyticus require enrichment for detecting 2 CFU/g of cells in the seafood sample [27]. The validation of an assay is important to confirm the sensitivity, specificity, and accuracy of the method.…”

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

“…Furthermore, Jiang et al. (2020) studied the CE value of IMPs for V. parahaemolyticus at different reaction time (30, 45, 60, 75, and 90 min). The results indicated that the CE significantly enhanced with the increase of time from 30 min (61.5%) to 45 min (81.2%).…”