Point-of-Need Diagnostics for Foodborne Pathogen Screening

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

“…Conventional laboratory-based analytical methods like high-performance liquid chromatography (HPLC), gas chromatography (GC), mass spectrometry (MS), enzyme-linked immunosorbent assay (ELISA) and real time polymerase chain reaction (qPCR) usually require complex and long procedures to obtain a result [ 1 , 2 , 3 ], but several situations often require a fast and on-site analyte detection [ 4 , 5 ]. As a consequence, in the last decades, the scientific research has been focused more and more on the development and optimization of portable, affordable, and user-friendly rapid methods of analysis for point-of-need (PON) testing [ 6 , 7 , 8 ].…”

Rapid detection methods for foodborne pathogens based on nucleic acid amplification: Recent advances, remaining challenges, and possible opportunities

“…Conversely, molecular methods, such as NAA-based methods, are more sensitivity and reliable compared to alternative detection techniques. To meet the demand for pathogen detection techniques that are affordable, sensitive and specific, user-friendly, rapid and robust, equipment-free, and deliverable to end users ( Campbell et al, 2021 ), future developments in this field should focus on simplifying, integrating, and miniaturizing NAA tools to enable on-site detection platforms for timely responses. Simplification is crucial to reduce the time required for sample pre-treatment/preparation, amplification, and readout detection.…”

Supra-Quantum Dot Assemblies to Maximize Color-Based Multiplexed Fluorescence Detection with a Smartphone Camera

“…Point-of-need diagnostics (PONDs) are sought for numerous applications in health care, food and water safety, environmental monitoring, and many other sectors. Smartphones are an emerging platform for PONDs because of their global ubiquity, ease of use, economy of scale, and capabilities such as connectivity, computation, on-board power, and built-in light sources and cameras .…”