Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon

Martin, María Victoria; Villarreal, Fernando; Miras, Isabelle; Navaza, A.; Haouz, Ahmed; González-Lebrero, Rodolfo M.; Kaufman, Sergio B.; Zabaleta, Eduardo

doi:10.1016/j.febslet.2009.09.055

Cited by 47 publications

(33 citation statements)

References 38 publications

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“…In chloroplasts, HCO 3 is converted into CO 2 , which servers as a substrate for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) [45]. Therefore, an increase in γCAL2 might be important for reusing the CO 2 produced in mitochondria through other biochemical pathways [46], and this protein is essential for regulating the carbon metabolism and for reducing the level of intracellular CO 2 , which might otherwise cause severe oxidative damage to the cell. An association between γCAL2 and an increased abundance of Rubisco and other photosynthetic proteins was also observed in the tolerant cultivar RB855536 (S1 Table).…”

Section: Discussionmentioning

confidence: 99%

Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots

et al. 2017

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Salt stress is one of the most common stresses in agricultural regions worldwide. In particular, sugarcane is affected by salt stress conditions, and no sugarcane cultivar presently show high productivity accompanied by a tolerance to salt stress. Proteomic analysis allows elucidation of the important pathways involved in responses to various abiotic stresses at the biochemical and molecular levels. Thus, this study aimed to analyse the proteomic effects of salt stress in micropropagated shoots of two sugarcane cultivars (CB38-22 and RB855536) using a label-free proteomic approach. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD006075. The RB855536 cultivar is more tolerant to salt stress than CB38-22. A quantitative label-free shotgun proteomic analysis identified 1172 non-redundant proteins, and 1160 of these were observed in both cultivars in the presence or absence of NaCl. Compared with CB38-22, the RB855536 cultivar showed a greater abundance of proteins involved in non-enzymatic antioxidant mechanisms, ion transport, and photosynthesis. Some proteins, such as calcium-dependent protein kinase, photosystem I, phospholipase D, and glyceraldehyde-3-phosphate dehydrogenase, were more abundant in the RB855536 cultivar under salt stress. Our results provide new insights into the response of sugarcane to salt stress, and the changes in the abundance of these proteins might be important for the acquisition of ionic and osmotic homeostasis during exposure to salt stress.

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Section: Discussionmentioning

confidence: 99%

Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots

et al. 2017

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“…A role for the CA/CAL proteins in an intracellular CO 2 transfer mechanism from mitochondria to chloroplasts analogous to the cyanobacterial carbon concentration mechanism has been proposed (Braun and Zabaleta, 2007). Recently, overexpressed CA2 was shown to bind CO 2 and/or bicarbonate efficiently (Martin et al, 2009). …”

Section: The Ca Subunits Of Complex I From Arabidopsismentioning

confidence: 99%

Internal Architecture of Mitochondrial Complex I from Arabidopsis thaliana

et al. 2010

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The NADH dehydrogenase complex (complex I) of the respiratory chain has unique features in plants. It is the main entrance site for electrons into the respiratory electron transfer chain, has a role in maintaining the redox balance of the entire plant cell and additionally comprises enzymatic side activities essential for other metabolic pathways. Here, we present a proteomic investigation to elucidate its internal structure. Arabidopsis thaliana complex I was purified by a gentle biochemical procedure that includes a cytochrome c-mediated depletion of other respiratory protein complexes. To examine its internal subunit arrangement, isolated complex I was dissected into subcomplexes. Controlled disassembly of the holo complex (1000 kD) by low-concentration SDS treatment produced 10 subcomplexes of 550, 450, 370, 270, 240, 210, 160, 140, 140, and 85 kD. Systematic analyses of subunit composition by mass spectrometry gave insights into subunit arrangement within complex I. Overall, Arabidopsis complex I includes at least 49 subunits, 17 of which are unique to plants. Subunits form subcomplexes analogous to the known functional modules of complex I from heterotrophic eukaryotes (the so-called N-, Q-, and P-modules), but also additional modules, most notably an 85-kD domain including g-type carbonic anhydrases. Based on topological information for many of its subunits, we present a model of the internal architecture of plant complex I.

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“…Although not active in vitro, gCA has been shown to bind inorganic carbon (Martin et al, 2009), affect complex I levels, plant growth, and gas-exchange rates when deleted (Perales et al, 2004;Soto et al, 2015), and cause plant sterility when ectopically overexpressed ). Arabidopsis has eight aCA genes, but only aCA1, aCA2, and aCA3 appear to be expressed in leaf tissue.…”

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confidence: 99%

The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO₂

et al. 2016

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Carbonic anhydrases (CAs) are zinc metalloenzymes that interconvert CO 2 and HCO 3 2 . In plants, both a-and b-type CAs are present. We hypothesize that cytoplasmic bCAs are required to modulate inorganic carbon forms needed in leaf cells for carbonrequiring reactions such as photosynthesis and amino acid biosynthesis. In this report, we present evidence that bCA2 and bCA4 are the two most abundant cytoplasmic CAs in Arabidopsis (Arabidopsis thaliana) leaves. Previously, bCA4 was reported to be localized to the plasma membrane, but here, we show that two forms of bCA4 are expressed in a tissue-specific manner and that the two proteins encoded by bCA4 localize to two different regions of the cell. Comparing transfer DNA knockout lines with wild-type plants, there was no reduction in the growth rates of the single mutants, bca2 and bca4. However, the growth rate of the double mutant, bca2bca4, was reduced significantly when grown at 200 mL L 21 CO 2 . The reduction in growth of the double mutant was not linked to a reduction in photosynthetic rate. The amino acid content of leaves from the double mutant showed marked reduction in aspartate when compared with the wild type and the single mutants. This suggests the cytoplasmic CAs play an important but not previously appreciated role in amino acid biosynthesis.Carbonic anhydrases (CAs) are zinc metalloenzymes that catalyze the interconversion of CO 2 and HCO 3 2 . Flowering plants possess members of the aCA, bCA, and gCA families. While all three CA families contain zinc, they clearly have evolved independently (Hewett-Emmett and Tashian, 1996). Most aCAs are monomeric, although there are notable exceptions (Whittington et al., 2001;Hilvo et al., 2008;Suzuki et al., 2010Suzuki et al., , 2011Cuesta-Seijo et al., 2011). The aCA active site contains a single zinc molecule coordinated by three His residues and a water molecule (Liljas et al., 1972). bCAs also contain a zinc active site, although the coordinating molecules are two Cys residues, a His, and a water molecule (Bracey et al., 1994). The active unit of the bCA is a dimer where the active site is located at the interface of the two monomers (Kimber and Pai, 2000). In contrast, gCAs are trimers that have their active site zinc ion situated at the interface of two subunits coordinated by His residues from both subunits (Kisker et al., 1996;Iverson et al., 2000).In Arabidopsis (Arabidopsis thaliana), there are three gCA proteins and two g-like proteins that interact to form an extra structure of complex I of the mitochondrial electron transport chain (Perales et al., 2004;Sunderhaus et al., 2006). Although not active in vitro, gCA has been shown to bind inorganic carbon (Martin et al., 2009), affect complex I levels, plant growth, and gas-exchange rates when deleted (Perales et al., 2004;Soto et al., 2015), and cause plant sterility when ectopically overexpressed ). Arabidopsis has eight aCA genes, but only aCA1, aCA2, and aCA3 appear to be expressed in leaf tissue. aCA1 has been reported to be localized to the c...

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Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon

Abstract: MINT‐7266036: gamma CA2 (uniprotkb:Q9C6B3) and gamma CA2 (uniprotkb:Q9C6B3) physically interact (MI:0914) by molecular sieving (MI:0071)

Cited by 47 publications

References 38 publications

Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots

Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots

Internal Architecture of Mitochondrial Complex I from Arabidopsis thaliana

The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO₂

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Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon

Abstract: MINT‐7266036: gamma CA2 (uniprotkb:Q9C6B3) and gamma CA2 (uniprotkb:Q9C6B3) physically interact (MI:0914) by molecular sieving (MI:0071)

Cited by 47 publications

References 38 publications

Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots

Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots

Internal Architecture of Mitochondrial Complex I from Arabidopsis thaliana

The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO2

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The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO₂