Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots

Abstract

Salt stress is one of the most common stresses in agricultural regions worldwide. In particular, sugarcane is affected by salt stress conditions, and no sugarcane cultivar presently show high productivity accompanied by a tolerance to salt stress. Proteomic analysis allows elucidation of the important pathways involved in responses to various abiotic stresses at the biochemical and molecular levels. Thus, this study aimed to analyse the proteomic effects of salt stress in micropropagated shoots of two sugarcane cultivars (CB38-22 and RB855536) using a label-free proteomic approach. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD006075. The RB855536 cultivar is more tolerant to salt stress than CB38-22. A quantitative label-free shotgun proteomic analysis identified 1172 non-redundant proteins, and 1160 of these were observed in both cultivars in the presence or absence of NaCl. Compared with CB38-22, the RB855536 cultivar showed a greater abundance of proteins involved in non-enzymatic antioxidant mechanisms, ion transport, and photosynthesis. Some proteins, such as calcium-dependent protein kinase, photosystem I, phospholipase D, and glyceraldehyde-3-phosphate dehydrogenase, were more abundant in the RB855536 cultivar under salt stress. Our results provide new insights into the response of sugarcane to salt stress, and the changes in the abundance of these proteins might be important for the acquisition of ionic and osmotic homeostasis during exposure to salt stress.

Potassium Synergize the Positive Effect of Ascorbic Acid on some Morpho-physiological Parameters of Salt Stressed Faba Bean Cultivars

“…Accumulation of soluble factions of carbohydrates and nitrogen compounds were considered as sign for the ability of certain genotype to tolerate salt stress; further increase in these components were detected when AsA was used (Ekmekçi & Karaman, 2012;Ejaz et al, 2012;Bastam et al, 2013;Billah et al, 2017). The accumulation of total sugars and proteins in response to salinity stress were documented in this work and others (Zhao et al, 2016;Passamani et al, 2017;Jini & Joseph, 2017), it may be due to the accumulation soluble fractions (El-Tayeb, 2005) and significant up-accumulation or downaccumulation of metabolites (Wu etal., 2013).…”

Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species

“…A large number of salt-responsive proteins have been isolated from some plant species by iTRAQ technique in the last few years. Some of these salt-responsive proteins have also been verified to play critical roles in plant salt tolerance 23 – 27 .…”

RESPOSTA GERMINATIVA DO FEIJÃO ARIGÓ E MANTEIGUINHA (Vinga unguicula L. Walp) SUBMETIDAS A CONDIÇÕES ESTRESSE SALINO

“…O crescimento vegetal sofre vários impactos negativos que são consequências do estresse salino, onde são capazes de promover mudanças em funções metabólicas, anatômica e fisiológicas das plantas (DUTRA et al, 2017). Passamani et al (2017) corroboram essa afirmação, explicando que os efeitos que ocorrem nas plantas são decorrentes de várias alterações tanto fisiológicas quanto moleculares um exemplo são as alterações do potencial osmótico, que impedem que raízes absorvam nutrientes e água, resultando no fechamento dos estômatos além de reduzir a atividade fotossintética.…”