Recombinant mycobacterial HSP65 in combination with incomplete Freund's adjuvant induced rat arthritis comparable with that induced by complete Freund's adjuvant

Yu, Yongli; Chen, Liang; Zhang, Peiyin; Wu, Xiuli; Zhang, Yongsheng; Yang, Ming; Di, Jing; Jiang, Hongyu; Wang, Liying

doi:10.1016/j.jim.2012.09.002

Cited by 4 publications

(3 citation statements)

References 28 publications

(26 reference statements)

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“…The inactivated mycobacterium is the main active substance in Freund's complete adjuvant and has been conventionally used to induce typical arthritis in rats. 19 Previous studies stated that malnutrition is the established symptom in RA, in this study we found the level of body weight was significantly decreased in the RA group compared with the control group (p < 0.05) probably because of overproduction of TNF-a and IL-1. 20 The nutritional status of RA patients was compromised despite adequate intake.…”

Section: Discussionsupporting

confidence: 56%

Goat milk CSN1S2 is able to decrease the severity scoring, TNF-a, and RAGE expression in complete Freund's adjuvant-induced rheumatoid arthritis model of rats

Bia

Virgirinia

Setiawan

et al. 2015

Biomarkers and Genomic Medicine

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This study aimed to elucidate whether CSN1S2 protein of goat's milk was able to inhibit pro-inflammatory cytokine [interleukin (IL)-17 and tumor necrosis factor (TNF)-a], RAGE, and caspase-3 expression in rheumatoid arthritis (RA) rats. A total of 24 Wistar female rats, were randomly assigned into four groups: control group (C), CSN1S2 protein of goat's milk group (CM), CFA-induced RA-rats group (RA), and the RA group treated by CSN1S2 protein of goat's milk (RAM). The severity of erythema and swelling in lower extremities were counted by scoring. IL-17, TNF-a, RAGE, and caspase-3 expression in synovial membranes were analyzed by confocal laser scanning microscopy (CLSM) and western blotting. Erythema and swelling in the RA group was significantly attenuated by goat's milk CSN1S2 (p < 0.05), but did not reach the level in the control group (p < 0.05). The use of CLSM, CSN1S2 protein of goat's milk could decrease the TNF-a, caspase-3, and the number of hyperplasia cells in comparison with the RA group (p < 0.05), to reach the level in the control group (p > 0.05). Western blotting analysis showed that the expression of IL-17, RAGE, TNF-a, and caspase-3 were higher in the RA group compared with the control group. CSN1S2 protein Please cite this article in press as: Bia RR, et al., Goat milk CSN1S2 is able to decrease the severity scoring, tumor necrosis factor-alpha, and advanced glycation end products receptor expression in complete Freund's adjuvant-induced rheumatoid arthritis model of rats, Biomarkers and Genomic Medicine (2015), http://dx.Biomarkers and Genomic Medicine (2015) xx, 1e8 of goat's milk decreased RAGE and TNF-a expression, but increased the IL-17 and caspase-3 expression. In conclusion, CSN1S2 protein of goat's milk decreased erythema, swelling, and inflammation in lower extremities. The CSN1S2 protein of goat's milk also decreased TNF-a and RAGE expression in the synovial membrane of ankle joints. Unfortunately, CSN1S2 protein of goat's milk may induce the production of IL-17. Therefore CSN1S2 protein of goat's milk may provide a nutritional therapy for attenuating the inflammation found in rheumatoid arthritis.

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Section: Discussionsupporting

confidence: 56%

Goat milk CSN1S2 is able to decrease the severity scoring, TNF-a, and RAGE expression in complete Freund's adjuvant-induced rheumatoid arthritis model of rats

Bia

Virgirinia

Setiawan

et al. 2015

Biomarkers and Genomic Medicine

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“…In an experiment of arthritis induction by Complete Freund’s adjuvant (CFA) replaced with the whole mycobacterium [72], the intradermal injection induced arthritic lesions at the same degree as CFA in ankle joints, with the production of anti-DNA and anti-Hsp65 in rats. Thus, the not remarkable increase of anti-Hsp65 antibodies presented by H III and L III mice may be responsible for the absence of disease.…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium leprae Hsp65 administration reduces the lifespan of aged high antibody producer mice

et al. 2014

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BackgroundAging process may result in immune modifications that lead to disruption of innate and acquired immunity mechanisms that may induce chronic-degenerative events. The heat shock proteins (Hsp), phylogeneticaly conserved among organisms, present as main function the ability of folding and refolding proteins, but they also are associated with chronic-degenerative disorders. Here were evaluated the role of M. leprae native Hsp65 (WT) and its point-mutated (K409A) on survival and anti-DNA and anti-Hsp65 antibody production of aged genetically selected mice for high (HIII) and low (LIII) antibody production; data from 120- and 270-days old mice (named “adult” or “aged”, respectively) were compared.ResultsWT Hsp65 administration induces reduction in the mean survival time of adult and aged female HIII mice, this effect being stronger in aged individuals. Surprisingly, the native protein administration increased the survival of aged female LIII when compared to K409A and control groups. No survival differences were observed in aged male mice after Hsp65 proteins inoculation. We observed increase in IgG1 anti-Hsp65 in WT and K409A aged HIII female mice groups and no marked changes in the anti-DNA (adult and aged HIII) and anti-Hsp65 IgG1 or IgG2a isotypes production in adult HIII female and aged male mice. LIII male mice presented increased anti-DNA and anti-Hsp65 IgG2a isotype production after WT or K409A injection, and LIII female groups showed no alterations.ConclusionsThe results revealed that the WT Hsp65 interferes with survival of aged HIII female mice without involvement of a remarkable IgG1 and IgG2a anti-DNA and anti-Hsp65 antibodies production. The deleterious effects of Hsp65 on survival time in aged HIII female mice could be linked to a gender-effect and are in agreement with those previously reported in lupus-prone mice.

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“…The AIA model was performed using methods previously reported in the literature. 21 Briey, male Lewis rats (weighing 200 g) were injected subcutaneously in the paw with 0.1 mL of Freund's complete adjuvant. The joint swelling was quantied by measuring perpendicularly with calipers.…”

Section: Induction Of Aiamentioning

confidence: 99%

Folic acid-conjugated glucose and dextran coated iron oxide nanoparticles as MRI contrast agents for diagnosis and treatment response of rheumatoid arthritis

Dai

Liu

et al. 2014

J. Mater. Chem. B

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Recombinant mycobacterial HSP65 in combination with incomplete Freund's adjuvant induced rat arthritis comparable with that induced by complete Freund's adjuvant

Cited by 4 publications

References 28 publications

Goat milk CSN1S2 is able to decrease the severity scoring, TNF-a, and RAGE expression in complete Freund's adjuvant-induced rheumatoid arthritis model of rats

Goat milk CSN1S2 is able to decrease the severity scoring, TNF-a, and RAGE expression in complete Freund's adjuvant-induced rheumatoid arthritis model of rats

Mycobacterium leprae Hsp65 administration reduces the lifespan of aged high antibody producer mice

Folic acid-conjugated glucose and dextran coated iron oxide nanoparticles as MRI contrast agents for diagnosis and treatment response of rheumatoid arthritis

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