Following on from our previous investigations, fluorescent core/shell CdTe@SiO 2 particles were prepared via the water-in-oil (W/O) reverse microemulsion method. It was found out that incubating the as-prepared aqueous CdTe quantum dots stabilized by thioglycolic acid in ammoniacal solution not only increased the fluorescence quantum yield of CdTe quantum dots but also gave rise to high retention of fluorescence throughout the silica coating. Under optimized conditions, the fluorescence quantum yield of CdTe quantum dots encapsulated in silica particles reached 47%. Both absorption and fluorescence spectroscopy were used in combination with X-ray photoelectron spectroscopy and electrophoresis to investigate the fluorescence enhancement effect occurring during incubation, the high retention of fluorescence quantum yield, as well as the formation of multicore/shell CdTe@SiO 2 particles as a result of the incubation process imposed on the as-prepared CdTe quantum dots.
The decreased expression of Dnmt1, Dnmt3a, Dnmt3b and Dnmt3L in oocytes (MII) and the change of genome-wide DNA methylation in oocytes and pre-implantation embryos due to aging may be related to lower reproductive potential in old female mice.
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