Recombinant expression, characterization, and application of a phospholipase B from Fusarium oxysporum

Su, Lingqia; Ji, Dening; Tao, Xiumei; Yu, Lingang; Wu, Jang‐Yen; Xia, Yongmei

doi:10.1016/j.jbiotec.2016.12.009

Cited by 11 publications

(15 citation statements)

References 46 publications

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“…On the one hand, phospholipases are of great medical and scientific interest because, for example, those from snake venom induce significant pharmacological effects such as inhibition of platelet aggregation, hypotension and antitumor activity [111]. On the other hand, the use of phospholipases in industrial applications is increasing due to the development of molecular techniques for the production of recombinant heterologous proteins and the production of new redesigned enzymes with improved catalytic properties, including chemical modification as a tool to improve their stability [112,113].…”

Section: 2-phospholipasesmentioning

confidence: 99%

Lecitase ultra: A phospholipase with great potential in biocatalysis

Virgen-Ortíz

Santos

Ortíz

et al. 2019

Molecular Catalysis

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Lecitase Ultra is a chimera produced by the fusion of the genes of the lipase from Thermomyces lanuginosus and the phospholipase A1 from Fusarium oxysporum. The enzyme was first designed for the enzymatic degumming of oils, as that problem was not fully resolved before. It is commercialized only as an enzyme solution by Novo Nordisk A/S. This review shows the main uses of this promising enzyme. Starting from the original degumming use, the enzyme has found applications in many other food modification applications, like production of structured phospholipids (e.g., derivatives of phosphatidylcholine), tuning the properties of flour, etc. Moreover, the enzyme has been used in fine chemistry (resolution of racemic mixtures), in the production of aromas and fragrances, polymers modification, etc. Some papers show the use of the enzyme in biodiesel production. Moreover, we present the different technologies applied to obtain a suitable immobilized biocatalyst, remarking the immobilization via interfacial activation and how heterofunctional acyl supports may solve some of the limitations. Immobilized enzyme physical and chemical modifications have also been presented. Finally, Lecitase Ultra has been one of the model enzymes in a new strategy to coimmobilize lipases and other less stable enzymes.

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Section: 2-phospholipasesmentioning

confidence: 99%

Lecitase ultra: A phospholipase with great potential in biocatalysis

Virgen-Ortíz

Santos

Ortíz

et al. 2019

Molecular Catalysis

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“…It had phospholipids hydrolyzing potential greater than its lipase capacity where it hydrolyzed the fatty acyl ester bond at the sn-1 and -2 positions of the phospholipids and reduced the oil phosphorus contents. This proved the potential industrial use of this PLB in oil degumming applications [172].…”

Section: Aromatic Carboxylic Acid Decarboxylasesmentioning

confidence: 59%

“…Phospholipase B Phospholipase B (PLB) hydrolyzes the phospholipid acyl groups to produce fatty acids and phosphoglycerates [171]. PLB is utilized to produce beneficial phospholipid derivatives, reduce food's cholesterol content, and refine vegetable oils, especially in terms of crude oil degumming [172]. Su et al, characterized a putative lipase from F. oxysporum NCBI-EGU84973.1 that was expressed in P. pastoris and classified as a PLB.…”

Section: Aromatic Carboxylic Acid Decarboxylasesmentioning

confidence: 99%

Bright Side of Fusarium oxysporum: Secondary Metabolites Bioactivities and Industrial Relevance in Biotechnology and Nanotechnology

Ibrahim

Sirwi

Eid

et al. 2021

JoF

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Fungi have been assured to be one of the wealthiest pools of bio-metabolites with remarkable potential for discovering new drugs. The pathogenic fungi, Fusarium oxysporum affects many valuable trees and crops all over the world, producing wilt. This fungus is a source of different enzymes that have variable industrial and biotechnological applications. Additionally, it is widely employed for the synthesis of different types of metal nanoparticles with various biotechnological, pharmaceutical, industrial, and medicinal applications. Moreover, it possesses a mysterious capacity to produce a wide array of metabolites with a broad spectrum of bioactivities such as alkaloids, jasmonates, anthranilates, cyclic peptides, cyclic depsipeptides, xanthones, quinones, and terpenoids. Therefore, this review will cover the previously reported data on F. oxysporum, especially its metabolites and their bioactivities, as well as industrial relevance in biotechnology and nanotechnology in the period from 1967 to 2021. In this work, 180 metabolites have been listed and 203 references have been cited.

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“…Previous reports on crude oil degumming mainly focused on the breeding of strains producing phospholipase A2, B, and C (Table 2). The phospholipases of the recombinant strains possess high application values in enzymatic degumming (Wei et al, 2015; Elena et al, 2017; Su et al, 2017). In this study, S. marcescens OM-PLA1 was effectively expressed in engineered P. pastoris .…”

Section: Discussionmentioning

confidence: 99%

Recombinant Expression of Serratia marcescens Outer Membrane Phospholipase A (A1) in Pichia pastoris and Immobilization With Graphene Oxide-Based Fe3O4 Nanoparticles for Rapeseed Oil Degumming

Yang

Jiang

et al. 2019

Front. Microbiol.

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Enzymatic degumming is an effective approach to produce nutritional, safe, and healthy refined oil. However, the high cost and low efficiency of phospholipase limit the application of enzymatic degumming. In this study, an 879 bp outer membrane phospholipase A (A1) (OM-PLA1) gene encoding 292 amino acid residues was isolated from the genome of Serratia marcescens . The recombinant OM-PLA1 profile of appropriately 33 KDa was expressed by the engineered Pichia pastoris GS115. The OM-PLA1 activity was 21.2 U/mL with the induction of 1 mM methanol for 72 h. The expression efficiencies of OM-PLA1 were 0.29 U/mL/h and 1.06 U/mL/OD600. A complex of magnetic graphene oxide (MGO) and OM-PLA1 (MGO-OM-PLA1) was prepared by immobilizing OM-PLA1 with graphene oxide-based Fe 3 O 4 nanoparticles by cross-linking with glutaraldehyde. The content of phosphorus decreased to 5.1 mg/kg rapeseed oil from 55.6 mg/kg rapeseed oil with 0.02% MGO-OM-PLA1 (w/w) at 50°C for 4 h. MGO-OM-PLA1 retained 51.7% of the initial activity after 13 times of continuous recycling for the enzymatic degumming of rapeseed oil. This study provided an effective approach for the enzymatic degumming of crude vegetable oil by developing a novel phospholipase and improving the degumming technology.

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Recombinant expression, characterization, and application of a phospholipase B from Fusarium oxysporum

Cited by 11 publications

References 46 publications

Lecitase ultra: A phospholipase with great potential in biocatalysis

Lecitase ultra: A phospholipase with great potential in biocatalysis

Bright Side of Fusarium oxysporum: Secondary Metabolites Bioactivities and Industrial Relevance in Biotechnology and Nanotechnology

Recombinant Expression of Serratia marcescens Outer Membrane Phospholipase A (A1) in Pichia pastoris and Immobilization With Graphene Oxide-Based Fe3O4 Nanoparticles for Rapeseed Oil Degumming

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