Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques

Steger, Krista; Valentine, P J; Heffron, Fred; So, Magdalene; Pauza, C. David

doi:10.1016/s0264-410x(98)00278-3

Cited by 18 publications

(16 citation statements)

References 26 publications

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“…As a consequence, less severe attenuations might be sufficient to prevent unwanted side effects which enhances the chance to retain immunogenicity (see below). In a recent primate example using an enteric serovar, rhesus macaques have successfully been vaccinated using attenuated S. typhimurium expressing an SIV antigen [26].…”

Section: Salmonella Serovarsupporting

confidence: 56%

“…It should be noted that with the exception of one trial ( S. typhi CVD908 expressing CSP), only humoral responses but not the important cell‐mediated immune responses against the heterologous antigen were monitored. Depending on the pathogen and the appropriate correlate of protection, measuring just antibody responses can considerable underestimate the efficacy of a vaccine as has been demonstrated, for example, for rhesus macaques receiving Salmonella expressing an antigen from SIV [26]. Nevertheless, further improvement of the immunogenicity of Salmonella ‐based vaccines for human use seems to be necessary.…”

Section: Discussionmentioning

confidence: 99%

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Recombinant liveSalmonellaspp. for human vaccination against heterologous pathogens

Bumann

Hueck²,

Aebischer

et al. 2000

FEMS Immunology &Amp; Medical Microbiology

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Section: Salmonella Serovarsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Recombinant liveSalmonellaspp. for human vaccination against heterologous pathogens

Bumann

Hueck²,

Aebischer

et al. 2000

FEMS Immunology &Amp; Medical Microbiology

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“…However, the confinement of Salmonella strains to the intracellular vesicular compartments of bacterially infected cells has limited their potential for stimulating major histocompatibility complex (MHC) class I-restricted CTL responses. Indeed, this may explain the inefficient stimulation of virus-specific CTL responses in earlier studies in which macaques were immunized with recombinant Salmonella strains engineered to express SIV antigens (13,37). To circumvent this problem, we have used an approach that takes advantage of the bacterial type III secretion system to deliver vaccine antigens directly into the cytoplasm and into the MHC class I antigen-processing pathway of Salmonella-infected cells (33).…”

mentioning

confidence: 99%

Mucosal Priming of Simian Immunodeficiency Virus-Specific Cytotoxic T-Lymphocyte Responses in Rhesus Macaques by theSalmonellaType III Secretion Antigen Delivery System

Evans

Chen

Gillis

et al. 2003

J Virol

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Nearly all human immunodeficiency virus (HIV) infections are acquired mucosally, and the gut-associated lymphoid tissues are important sites for early virus replication. Thus, vaccine strategies designed to prime virus-specific cytotoxic T lymphocyte (CTL) responses that home to mucosal compartments may be particularly effective at preventing or containing HIV infection. The Salmonella type III secretion system has been shown to be an effective approach for stimulating mucosal CTL responses in mice. We therefore tested ⌬phoP-phoQ attenuated strains of Salmonella enterica serovar Typhimurium and S. enterica serovar Typhi expressing fragments of the simian immunodeficiency virus (SIV) Gag protein fused to the type III-secreted SopE protein for the ability to prime virus-specific CTL responses in rhesus macaques. Mamu-A*01 ؉ macaques were inoculated with three oral doses of recombinant Salmonella, followed by a peripheral boost with modified vaccinia virus Ankara expressing SIV Gag (MVA Gag). Transient low-level CTL responses to the Mamu-A*01 Gag 181-189 epitope were detected following each dose of Salmonella. After boosting with MVA Gag, strong Gag-specific CTL responses were consistently detected, and tetramer staining revealed the expansion of Gag 181-189 -specific CD8 ؉ T-cell responses in peripheral blood. A significant percentage of the Gag 181-189 -specific T-cell population in each animal also expressed the intestinal homing receptor ␣4␤7. Additionally, Gag 181-189 -specific CD8 ؉ T cells were detected in lymphocytes isolated from the colon. Yet, despite these responses, Salmonella-primed/MVA-boosted animals did not exhibit improved control of virus replication following a rectal challenge with SIVmac239. Nevertheless, this study demonstrates the potential of mucosal priming by the Salmonella type III secretion system to direct SIV-specific cellular immune responses to the gastrointestinal mucosa in a primate model.

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“…Expression systems based on salmonella are another example of a bacterial vector in use in AIDS [162][163][164][165][166]. SIV-specific immunity is induced using salmonella-based vaccine, but the protection is not achieved even when relatively "easy" challenge with SHIV89.6P is used [166].…”

Section: Bacteria-based Vaccinesmentioning

confidence: 99%

Utility of Nonhuman Primate Models for Vaccines

Marx

Voevodin²

2012

Vaccinology

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Recombinant, attenuated Salmonella typhimurium stimulate lymphoproliferative responses to SIV capsid antigen in rhesus macaques

Cited by 18 publications

References 26 publications

Recombinant liveSalmonellaspp. for human vaccination against heterologous pathogens

Recombinant liveSalmonellaspp. for human vaccination against heterologous pathogens

Mucosal Priming of Simian Immunodeficiency Virus-Specific Cytotoxic T-Lymphocyte Responses in Rhesus Macaques by theSalmonellaType III Secretion Antigen Delivery System

Utility of Nonhuman Primate Models for Vaccines

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