Recombinant live<i>Salmonella</i>spp. for human vaccination against heterologous pathogens

Bumann, Dirk; Hueck, Christoph J.; Aebischer, Toni; Meyer, Thomas F.

doi:10.1111/j.1574-695x.2000.tb01450.x

Cited by 52 publications

(4 citation statements)

References 60 publications

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“…In addition, differences in immunogenicity between fur mutants of S. Typhimurium and S. Gallinarum may also be explained by the fact that the disease caused by S. Typhimurium in mice is not exactly the same as that caused by S. Gallinarum in chickens. Support for this view comes from observations that mutations that completely attenuate S. Typhimurium are often insufficiently attenuating for S. enterica serovar Typhi in humans (47,48). Since S. Gallinarum and S. Typhi are strictly host-adapted serovars, the mechanisms of their pathogenesis are different from those of the broad-host range S. Typhimurium.…”

Section: Discussionmentioning

confidence: 95%

Evaluation of Protective Efficacy of Live Attenuated Salmonella enterica Serovar Gallinarum Vaccine Strains against Fowl Typhoid in Chickens

Łaniewski

Mitra

Khan

et al. 2014

Clin. Vaccine Immunol.

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c Salmonella enterica serovar Gallinarum is the etiological agent of fowl typhoid, which constitutes a considerable economic problem for poultry growers in developing countries. The vaccination of chickens seems to be the most effective strategy to control the disease in those areas. We constructed S. Gallinarum strains with a deletion of the global regulatory gene fur and evaluated their virulence and protective efficacy in Rhode Island Red chicks and Brown Leghorn layers. The fur deletion mutant was avirulent and, when delivered orally to chicks, elicited excellent protection against lethal S. Gallinarum challenge. It was not as effective when given orally to older birds, although it was highly immunogenic when delivered by intramuscular injection. We also examined the effect of a pmi mutant and a combination of fur deletions with mutations in the pmi and rfaH genes, which affect O-antigen synthesis, and ansB, whose product inhibits host T-cell responses. The S. Gallinarum ⌬pmi mutant was only partially attenuated, and the ⌬ansB mutant was fully virulent. The ⌬fur ⌬pmi and ⌬fur ⌬ansB double mutants were attenuated but not protective when delivered orally to the chicks. However, a ⌬pmi ⌬fur strain was highly immunogenic when administered intramuscularly. All together, our results show that the fur gene is essential for the virulence of S. Gallinarum, and the fur mutant is effective as a live recombinant vaccine against fowl typhoid.

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Section: Discussionmentioning

confidence: 95%

Evaluation of Protective Efficacy of Live Attenuated Salmonella enterica Serovar Gallinarum Vaccine Strains against Fowl Typhoid in Chickens

Łaniewski

Mitra

Khan

et al. 2014

Clin. Vaccine Immunol.

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“…Since orally administered live attenuated Salmonella spp. that express heterologous antigens are safe and highly immunogenic, they are promising candidates; they can elicit prolonged, protective, systemic, and mucosal immune responses against the heterologous pathogen (67). …”

Section: Live Non-pathogenic Vaccinesmentioning

confidence: 99%

Live Vaccination Tactics: Possible Approaches for Controlling Visceral Leishmaniasis

2014

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Vaccination with durable immunity is the main goal and fundamental to control leishmaniasis. To stimulate the immune response, small numbers of parasites are necessary to be presented in the mammalian host. Similar to natural course of infection, strategy using live vaccine is more attractive when compared to other approaches. Live vaccines present the whole spectrum of antigens to the host immune system in the absence of any adjuvant. Leishmanization was the first effort for live vaccination and currently used in a few countries against cutaneous leishmaniasis, in spite of their obstacle and safety. Then, live attenuated vaccines developed with similar promotion of creating long-term immunity in the host with lower side effect. Different examples of attenuated strains are generated through long-term in vitro culturing, culturing under drug pressure, temperature sensitivity, and chemical mutagenesis, but none is safe enough and their revision to virulent form is possible. Attenuation through genetic manipulation and disruption of virulence factors or essential enzymes for intracellular survival are among other approaches that are intensively under study. Other designs to develop live vaccines for visceral form of leishmaniasis are utilization of live avirulent microorganisms such as Lactococcus lactis, Salmonella enterica, and Leishmania tarentolae called as vectored vaccine. Apparently, these vaccines are intrinsically safer and can harbor the candidate antigens in their genome through different genetic manipulation and create more potential to control Leishmania parasite as an intracellular pathogen.

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“…In almost all cases, this evidence indicates expression of the respective antigens at least at some stage of the infection. Virulence phenotypes in human beings are also available in a few cases from vaccine trials with live attenuated Salmonella strains (20–22). These scarce human data are largely consistent with observations for the corresponding Salmonella mutants in the mouse model, but systematic comparisons are currently impossible due to the lack of human data for most potential virulence factors.…”

Section: Antigen Expression In Host Tissuesmentioning

confidence: 99%

Identification of Protective Antigens for Vaccination against Systemic Salmonellosis

Bumann

2014

Front. Immunol.

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There is an urgent medical need for improved vaccines with broad serovar coverage and high efficacy against systemic salmonellosis. Subunit vaccines offer excellent safety profiles but require identification of protective antigens, which remains a challenging task. Here, I review crucial properties of Salmonella antigens that might help to narrow down the number of potential candidates from more than 4000 proteins encoded in Salmonella genomes, to a more manageable number of 50–200 most promising antigens. I also discuss complementary approaches for antigen identification and potential limitations of current pre-clinical vaccine testing.

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Recombinant liveSalmonellaspp. for human vaccination against heterologous pathogens

Cited by 52 publications

References 60 publications

Evaluation of Protective Efficacy of Live Attenuated Salmonella enterica Serovar Gallinarum Vaccine Strains against Fowl Typhoid in Chickens

Evaluation of Protective Efficacy of Live Attenuated Salmonella enterica Serovar Gallinarum Vaccine Strains against Fowl Typhoid in Chickens

Live Vaccination Tactics: Possible Approaches for Controlling Visceral Leishmaniasis

Identification of Protective Antigens for Vaccination against Systemic Salmonellosis

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