Recognition of <i>Helicobacter pylori</i> by protein‐targeting aptamers

Yan, Wanli; Gu, Lide; Ren, Wei; Ma, Xiaoyi; Qin, Mingcan; Lyu, Mingsheng; Wang, Shujun

doi:10.1111/hel.12577

Cited by 16 publications

(8 citation statements)

References 49 publications

(87 reference statements)

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“…Hp4 was an aptamer screened by our laboratory using the H. pylori surface protein HP-Ag as a target. 64 The binding rate of Hp4 and HPA-2 was then compared. The two aptamers were incubated with the same number of H. pylori cells at the same concentration (200 nM).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA Aptamers

Wu¹,

Gu²,

Ma³

et al. 2021

ACS Omega

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Helicobacter pylori was first isolated from gastritis patients by Barry J. Marshall and J. Robin Warren in 1982, and more than 90% of duodenal ulcers and about 80% of gastric ulcers are caused by H. pylori infection. Most detection methods require sophisticated instruments and professional operators, making detection slow and expensive. Therefore, it is critical to develop a simple, fast, highly specific, and practical strategy for the detection of H. pylori. In this study, we used H. pylori as a target to select unique aptamers that can be used for the detection of H. pylori. In our study, we used random ssDNA as an initial library to screen nucleic acid aptamers for H. pylori. We used binding rate and the fluorescence intensity to identify candidate aptamers. One DNA aptamer, named HPA-2, was discovered through six rounds of positive selection and three rounds of negative selection, and it had the highest affinity constant of all aptamers tested (K d = 19.3 ± 3.2 nM). This aptamer could be used to detect H. pylori and showed no specificity for other bacteria. Moreover, we developed a new sensor to detect H. pylori with the naked eye for 5 min using illumination from a hand-held flashlight. Our study provides a framework for the development of other aptamer-based methods for the rapid detection of pathogenic bacteria.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…Hp4 was an aptamer screened by our laboratory using the H. pylori surface protein HP-Ag as a target . The binding rate of Hp4 and HPA-2 was then compared.…”

Section: Methodsmentioning

confidence: 99%

Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA Aptamers

Wu¹,

Gu²,

Ma³

et al. 2021

ACS Omega

Self Cite

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“…Compared to antibodies, aptamers are more stable and possess long-term stability. Wanli Yan et al [ 98 ] emphasized that Hp4 aptamer exhibited high specificity for H. pylori cells, without any specificity for other bacteria.…”

Section: Biosensorsmentioning

confidence: 99%

Evolution of Diagnostic Methods for Helicobacter pylori Infections: From Traditional Tests to High Technology, Advanced Sensitivity and Discrimination Tools

et al. 2022

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Rapid diagnosis and treatment application in the early stages of H. pylori infection plays an important part in inhibiting the transmission of this infection as this bacterium is involved in various gastric pathologies such as gastritis, gastro-duodenal ulcer, and even gastric neoplasia. This review is devoted to a quick overview of conventional and advanced detection techniques successfully applied to the detection of H. pylori in the context of a compelling need to upgrade the standards of the diagnostic methods which are currently being used. Selecting the best diagnostic method implies evaluating different features, the use of one or another test depending on accessibility, laboratories equipment, and the clinical conditions of patients. This paper aims to expose the diagnosis methods for H. pylori that are currently available, highlighting their assets and limitations. The perspectives and the advantages of nanotechnology along with the concept of nano(bio)sensors and the development of lab-on-chip devices as advanced tools for H. pylori detection, differentiation, and discrimination is also presented, by emphasizing multiple advantages: simple, fast, cost-effective, portable, miniaturized, small volume of samples required, highly sensitive, and selective. It is generally accepted that the development of intelligent sensors will completely revolutionize the acquisition procedure and medical decision in the framework of smart healthcare monitoring systems.

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“…Fluorescence biosensors Since fluorophores can be easily modified with aptamers, aptamer-based fluorescent methods usually harness aptamer-modified fluorophores for fluorescence signal transduction based on the binding of the target and aptamers, with the characteristics of rapidness and high sensitivity [74]. A variety of fluorophores with different properties can be incorporated, thereby boosting the applications of aptamer-based fluorescent sensors especially in food safety [75] and public health [76]. For example, Song et al employed an aptamer-based strategy for the quantification of S. aureus using the fluorescence resonance energy transfer (FRET) principle (Fig.…”

Section: Bacterial Detectionmentioning

confidence: 99%

Selection and applications of functional nucleic acids for infectious disease detection and prevention

et al. 2021

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Infectious diseases caused by pathogenic microorganisms such as viruses and bacteria pose a great threat to human health. Although a significant progress has been obtained in the diagnosis and prevention of infectious diseases, it still remains challenging to develop rapid and cost-effective detection approaches and overcome the side effects of therapeutic agents and pathogen resistance. Functional nucleic acids (FNAs), especially the most widely used aptamers and DNAzymes, hold the advantages of high stability and flexible design, which make them ideal molecular recognition tools for bacteria and viruses, as well as potential therapeutic drugs for infectious diseases. This review summarizes important advances in the selection and detection of bacterial-and virus-associated FNAs, along with their potential prevention ability of infectious disease in recent years. Finally, the challenges and future development directions are concluded.

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Recognition of Helicobacter pylori by protein‐targeting aptamers

Cited by 16 publications

References 49 publications

Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA Aptamers

Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA Aptamers

Evolution of Diagnostic Methods for Helicobacter pylori Infections: From Traditional Tests to High Technology, Advanced Sensitivity and Discrimination Tools

Selection and applications of functional nucleic acids for infectious disease detection and prevention

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