Recognition of Amphiphiles with Many Pendent Galactose Residues byRicinus communisAgglutinin

Community-Based Network Study of Protein-Carbohydrate Interactions in Plant Lectins Using Glycan Array Data

“…?RCA is a glycoprotein from seeds of castor plants and one of the most important applied lectins that have been widely used as a tool to study cell surfaces and to purify glycans [79]. RCA promotes binding and agglutination of polysaccharides and glycoproteins in addition to liposomes and micelles containing glycolipids with galactosyl residues [80], [81]. Furthermore, the specificities of interactions of RCA with neutral and sialylated oligosaccharides have been well established and is consistent with our results as summarized in Table S11 [82].…”

Kinetic study on the binding of lectin to mannose residues in a polymer brush

“…It has very often been reported that lectin does not induce a coagulation of liposomes which incorporate sugar-carrying amphiphiles with a short spacer group between the sugar residue and a hydrophobic anchor [51][52][53]. This is due to a large steric hindrance preventing the huge lectin molecule from approaching the small sugar residues on the membrane surface.…”

Interaction of wheat germ agglutinin with an N-acetylglucosamine-carrying telomer brush accumulated on a colloidal gold monolayer

“…An azo-type radical initiator with dioctadecyl groups at both ends (DODA-501, Scheme 1(A)) [45][46][47][48] and the disulfidecarrying photo-iniferter (Cys-BDC, Scheme 1(B)) [23] were prepared as described earlier. A polymerizable phospholipid (bis(trans, trans-2,4-dioctadecadienoyl)phosphatidyl choline, DDPC) and 3-aminopropyl triethoxysilane (APTS) were purchased from Japan Fat & Oil Co., Tokyo, Japan and ShinEtsu Chemicals, Tokyo, Japan, respectively.…”

“…1(B)) [46][47][48]. The turbidity change of GlcNAc-carrying liposomes caused by the addition of WGA was dependent on the DP value of DODA-PMHGlcNAc: The addition of WGA to the dispersion of DODA-PMHGlcNAc (DP = 19) liposome induced a gradual increase in turbidity at 350 nm.…”

“…), it has often been reported that a lectin does not bind to an incorporated sugar-carrying amphiphile with a short spacer group between the sugar residue and a hydrophobic anchor [46][47][48], due to a large steric hindrance preventing the huge lectin from approaching the small sugar residue on the membrane surface. Therefore, CysPMHGlcNAc, which has a telomer chain with many pendent GlcNAc groups, was adopted as a "fly" to fish for WGA in this study.…”