Interaction of wheat germ agglutinin with an N-acetylglucosamine-carrying telomer brush accumulated on a colloidal gold monolayer

Kitano, Hiromi; Nakada, Hideharu; Mizukami, Kazuya

doi:10.1016/j.colsurfb.2007.06.028

Cited by 19 publications

(11 citation statements)

References 59 publications

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“…63 In this last case, the labile initiator fragment confers to the polymer chain degradability at a precise location, allowing the controlled reduction in molecular weight and the degree of branching, but also the formation of certain functional endgroups. Regarding reduction in size and degree of branching upon degradation, polymers made using degradable initiators have been transformed to smaller entities of simpler architecture, with most notable examples the scission of linear homopolymers into two equal pieces, 42,45,47,52,53,60 the conversion of ABA triblocks into diblocks, 43,44,51 the decomposition of star polymers into the constituting arms, [48][49][50] the separation of dumbbell polymers to two star polymers, 46,54 and the conversion of homopolymer model networks to star homopolymers. 57,59,61,62 Regarding the formation of functional end-groups, these are the residues of the labile initiator fragment after its degradation, remaining in the polymer.…”

Section: Introductionmentioning

confidence: 99%

End-linked, amphiphilic, degradable polymer conetworks: synthesis by sequential atom transfer radical polymerization using a bifunctional, cleavable initiator

et al. 2012

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Section: Introductionmentioning

confidence: 99%

End-linked, amphiphilic, degradable polymer conetworks: synthesis by sequential atom transfer radical polymerization using a bifunctional, cleavable initiator

et al. 2012

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“…Galactose presenting polymer brushes was also used to modify colloidal gold monolayers and their binding of the lectin RCA120 was followed by LSPR, and the binding of HepG2 cells which contain galactose receptors was followed by optical microscopy [98]. The interaction of wheat germ agglutinin (WGA) with a disulfidemodified telomer polymer on a colloidal gold monolayer was also followed by LSPR [99].…”

Section: Binding Affinitymentioning

confidence: 99%

Self-Assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces

Bhattarai¹,

Neupane²,

Mikhaylov³

et al. 2017

Carbohydrate

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Self-assembled monolayers (SAMs) presenting carbohydrates (glycans) have been widely prepared on gold surfaces to mimic the carbohydrate surfaces that are involved in molecular recognition phenomena in living cells. The binding affinity of carbohydrate immbolized on SAM surfaces to various carbohydrate-binding proteins (such as lectins) can be studied by optical, electrochemical, piezoelectrical and thermal sensing techniques. The lectins present on the surface of pathogens (e.g., bacteria or viruses) can be used as targets for capturing onto carbohydrates immobilized on SAM surfaces. The immobilized carbohydrates can also be used for detecting different types of disease biomarkers present in bodily fluids. Synergistic properties of carbohydrate SAMs and gold nanoparticles can be used for vaccine preparation and drug delivery. By studying different types of glycans, their properties, and the behavior toward recognition of specific pathogens and biomarkers, we can develop not only new therapeutics but also enhance the diagnostic strategies of various diseases. In this chapter, we discuss carbohydrate-terminated SAMs and their common preparation strategies. Next, we focus on roles of different components of SAMs, characterization techniques, and applications.

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“…10.14.4), which is used in Support Protocol 1 for capping failure sequences in ODN synthesis. The synthesis has been reported previously (Kitano et al, 2008;Zhu et al, 2010;Fang et al, 2011). Prepare methacryloyl alcohol S.12 1.…”

Section: Synthesis Of Polymerizable Phosphoramidite For Tagging Failumentioning

confidence: 99%

Purification of Synthetic Oligonucleotides via Catching by Polymerization

Fang

Fueangfung

Yuan

2012

CP Nucleic Acid Chemistry

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This unit describes the purification of synthetic oligodeoxyribonucleotides (ODN) using a catching-by-polymerization approach. In a crude ODN, the major impurity is the failure sequences generated in the coupling step of each synthetic cycle. They are difficult to remove due to the similarity of their physical properties to the full-length sequences. Two non-chromatographic methods are described in the unit to solve the problem. In the first one, during automated synthesis, the failure sequences are tagged with a methacrylamide group, which is polymerizable and can participate in acrylamide radical polymerization reactions; the full-length sequences are not tagged. After synthesis, the crude mixture is subjected to polymerization. The failure sequences are incorporated into an insoluble polymer; the full-length sequences are extracted with water. In the second method, the full-length sequences are tagged with a methacrylamide group via a cleavable linker; the failure sequences are not tagged. After synthesis, the full-length sequences are incorporated into a polymer; the failure sequences are washed away with water. Pure full-length sequences are cleaved from the polymer. The two methods are complementary. .

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Interaction of wheat germ agglutinin with an N-acetylglucosamine-carrying telomer brush accumulated on a colloidal gold monolayer

Cited by 19 publications

References 59 publications

End-linked, amphiphilic, degradable polymer conetworks: synthesis by sequential atom transfer radical polymerization using a bifunctional, cleavable initiator

End-linked, amphiphilic, degradable polymer conetworks: synthesis by sequential atom transfer radical polymerization using a bifunctional, cleavable initiator

Self-Assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces

Purification of Synthetic Oligonucleotides via Catching by Polymerization

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