Kinetic study on the binding of lectin to mannose residues in a polymer brush

Kitano, Hiromi; Takahashi, Yoshiko; Mizukami, Kazuya; Matsuura, Kazuhiro

doi:10.1016/j.colsurfb.2008.12.016

Cited by 33 publications

(25 citation statements)

References 63 publications

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“…We have found that K d for the mannosides and Con A interactions are in the nanomolar range and are slightly smaller than previously reported values for mannose–Con A interaction using SPR ( K d =135–400 nM) [47] and QCM ( K d = 250–400 nM) techniques [48, 49]. It has also been found that binding affinity of multivalent ligands to lectins are stronger compared to monovalent ligands [50, 51].…”

Section: Resultscontrasting

confidence: 51%

Electrochemical impedance spectroscopy study of Concanavalin A binding to self-assembled monolayers of mannosides on gold wire electrodes

Bhattarai

Tan

Pandey

et al. 2016

Journal of Electroanalytical Chemistry

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The interactions of the lectin Concanavalin A (Con A) with self-assembled monolayers (SAMs) of thiolated mono-, di-, and tri-mannosides were studied on the surface of gold wires using electrochemical impedance spectroscopy (EIS). The SAMs of mannosides were prepared either pure or along with thiolated triethylene glycol (TEG) at different molar ratios (1:1, 1:2, 1:4, 1:9, and 1:19) to better understand and optimize the interaction conditions. The charge-transfer resistance of the [Fe(CN)6]3−/4− redox probe was compared before and after the interaction at different concentrations of Con A to determine the equilibrium dissociation constant (Kd) and limit of detection (LOD). Values of Kd were found in the nanomolar range showing multivalent interactions between mannosides and Con A, and LOD was found ranging from 4–13 nM depending on the type of mannoside SAM used. Analysis using the Hill equation suggests negative cooperativity in the binding behavior. Peanut agglutinin was used as a negative control, and cyclic voltammetry was used to further support the experiments. We have found that neither the pure nor the widely dispersed monolayers of mannosides provide the conditions for optimal binding of Con A. The binding of Con A to these SAMs is sensitive to the molar ratio of the mannoside used to prepare the SAM and to the structure of the mannoside. A simple cleaning method has also been shown to regenerate the used gold wire electrodes. The results from these experiments contribute to the development of simple, cheap, selective, and sensitive EIS-based bioassays, especially for lectin–carbohydrate interactions.

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Section: Resultscontrasting

confidence: 51%

Electrochemical impedance spectroscopy study of Concanavalin A binding to self-assembled monolayers of mannosides on gold wire electrodes

Bhattarai

Tan

Pandey

et al. 2016

Journal of Electroanalytical Chemistry

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“…50,51,53 Values of k on = 2.0 × 10 4 M −1 s −1 and k off = 2.6 × 10 −3 s −1 corresponding to K d = 130 nM were reported for Con A interacting with SAMs of a thiolated PEG linker terminated in a mannose unit on 5 nm Au islands. 53 As noted by Bellapadrona et al, a range of values of k off between 2.48 × 10 −4 s −1 and 1.2 × 10 −2 s −1 , and of k on ranging from 5.2 × 10 3 M −1 s −1 to 1.4 × 10 5 M −1 s −1 , corresponding to K d values ranging from 42 nM to 423 nM have been reported for the Con A–mannose interaction depending upon measurement method, details of surface modification, and experimental conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Au nanoparticles supported on glass have been modified by polymer brushes with many pendant glucose residues and LSPR was used to determine a binding constant from real-time analysis of 5.0 ± 0.2 × 10 5 M −1 noted as larger than that for Con A binding to methyl α-D-glucopyranoside of 2.4 ± 0.1 × 10 3 M −1 in solution and attributed to multipoint binding effects. 50 The use of supported gold nanoparticles modified with a polymer brush having pendant mannose units was applied to follow Con A binding, 51 resulting in an apparent association constant determined from analysis of real-time association kinetics data of 7.4 ± 0.1 × 10 6 M −1 , noted as much greater than that for Con A tomethyl α-D-mannopyranoside in solution of 7.6 ± 0.2 × 10 3 M −1 , with the difference attributed to multipoint binding effects. Au nanoparticles bound to glass modified by 3-aminopropyltrimethoxysilane were modified by dodecanethiol SAMs into which a N -acetylglucosamine glycolipid was inserted, and shift in the LSPR peak wavelength due to Con A binding was observed in transmission mode in real-time for both association and dissociation.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical synthesis of nanostructured gold film for the study of carbohydrate–lectin interactions using localized surface plasmon resonance spectroscopy

Bhattarai

Sharma

Fujikawa

et al. 2015

Carbohydrate Research

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Localized surface plasmon resonance (LSPR) spectroscopy is a label-free chemical and biological molecular sensing technique whose sensitivity depends upon development of nanostructured transducers. Herein, we report an electrodeposition method for fabricating nanostructured gold films (NGFs) that can be used as transducers in LSPR spectroscopy. The NGF was prepared by electrodepositing gold from potassium dicyanoaurate solution onto a flat gold surface using two sequential controlled potential steps. Imaging by scanning electron microscopy reveals a morphology consisting of randomly configured block-like nanostructures. The bulk refractive index sensitivity of the prepared NGF is 100 ± 2 nm RIU−1 and the initial peak in the reflectance spectrum is at 518 ± 1 nm under N2(g). The figure of merit is 1.7. In addition, we have studied the interaction between carbohydrate (mannose) and lectin (Concanavalin A) on the NGF surface using LSPR spectroscopy by measuring the interaction of 8-mercaptooctyl-α-D-mannopyranoside (αMan-C8-SH) with Concanavalin A by first immobilizing αMan-C8-SH in mixed SAMs with 3,6-dioxa-8-mercaptooctanol (TEG-SH) on the NGF surface. The interaction of Con A with the mixed SAMs is confirmed using electrochemical impedance spectroscopy. Finally, the NGF surface was regenerated to its original sensitivity by removing the SAM and the bound biomolecules. The results from these experiments contribute toward the development of inexpensive LSPR based sensors that could be useful for studying glycan–protein interactions and other bioanalytical purposes.

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“…Interestingly, the membrane could be regenerated upon acid treatment ("acid leaching method") without any degradation. Kitano et al [128,134] reported the synthesis of thiol-terminated glycopolymers (Entry 100 and 116; Table 2) and their grafting on a colloidal gold-immobilized glass substrate for the study of binding and adsorption processes by UV-Vis spectrophotometer with the help of a localized surface plasmon resonance. To this end, 2-(2-bromoisobutyroyloxy)ethyl disulfide A34 was used to initiated the polymerization of glucoside methacrylate M34a and mannoside methacrylate derivatives with M n = 1800-8600 Da).…”

Section: Scheme 14mentioning

confidence: 99%

Synthesis of Glycopolymer Architectures by Reversible-Deactivation Radical Polymerization

Ghadban

Albertin

2013

Polymers

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This review summarizes the state of the art in the synthesis of well-defined glycopolymers by Reversible-Deactivation Radical Polymerization (RDRP) from its inception in 1998 until August 2012. Glycopolymers architectures have been successfully synthesized with four major RDRP techniques: Nitroxide-mediated radical polymerization (NMP), cyanoxyl-mediated radical polymerization (CMRP), atom transfer radical polymerization (ATRP) and reversible addition-fragmentation chain transfer (RAFT) polymerization. Over 140 publications were analyzed and their results summarized according to the technique used and the type of monomer(s) and carbohydrates involved. Particular emphasis was placed on the experimental conditions used, the structure obtained (comonomer distribution, topology), the degree of control achieved and the (potential) applications sought. A list of representative examples for each polymerization process can be found in tables placed at the beginning of each section covering a particular RDRP technique.

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Kinetic study on the binding of lectin to mannose residues in a polymer brush

Cited by 33 publications

References 63 publications

Electrochemical impedance spectroscopy study of Concanavalin A binding to self-assembled monolayers of mannosides on gold wire electrodes

Electrochemical impedance spectroscopy study of Concanavalin A binding to self-assembled monolayers of mannosides on gold wire electrodes

Electrochemical synthesis of nanostructured gold film for the study of carbohydrate–lectin interactions using localized surface plasmon resonance spectroscopy

Synthesis of Glycopolymer Architectures by Reversible-Deactivation Radical Polymerization

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