Recent trends in fluorescent aptasensors for mycotoxin detection in food: Principles, constituted elements, types, and applications

Abstract

Mycotoxins are the secondary metabolites of fungi in food and can be toxic to people. Therefore, developing simple, sensitive, and specific detection methods to detect mycotoxins in food is essential for ensuring food safety. Fluorescent aptasensors for the detection of mycotoxins in food have developed rapidly. They are simple and fast with high affinity and specificity. However, fluorescent aptasensors have not yet been applied in officially approved standards so far. This review elucidates fluorescent aptasensors for mycotoxin detection, including principles, constituted elements, types, and applications. Screening results of aptamers and each aptamer sequence used in fluorescent aptasensors are summarized to facilitate further research for later studies.We also focused on fluorescent probes, nanomaterials, and other strategies in the process of constructing fluorescent aptasensors. We then discuss current challenges and future perspectives in fluorescent aptasensors for mycotoxin detection.

Emerging applications of metabolomics in food science and future trends

“…The use of metabolomics techniques allows for the rapid quantification or localisation of certain types of substances in foods, which is a very convenient method in food composition studies. Based on this same principle, toxins, hormones, pesticides or drug residues in food can be identified for the purpose of food safety control ( Yan et al, 2022 ). Flavour components of food products are also a popular research topic.…”

Selection and Characterization of DNA Aptamers for Constructing Aptamer-AuNPs Colorimetric Method for Detection of AFM1

“…[ 13 , 14 ]. In recent years, many research groups have screened and prepared aptamers for a variety of potential hazards in food matrices, such as biotoxins [ 15 , 16 ], pesticides [ 17 ], veterinary drugs [ 18 ], pathogenic microorganisms [ 19 ], and heavy metals [ 20 ]. As biological recognition components, aptamers have been widely used in the analysis of hazardous substances in food safety, showing outstanding detection performance, such as high sensitivity, simple operation, fast detection speed, and low cost.…”

Easy-to-Use Visual Sensing System for Milk Freshness, Sensitized with Acidity-Responsive N-Doped Carbon Quantum Dots

“…Recently, fluorescence sensing methods have been widely used for food assessment, owing to their high operational simplicity, high sensitivity, and short response time [10,11]; however, these fluorescence methods need to be improved, owing to the requirement of specialized instruments [12]. Eye-readable sensors have attracted increasing attention for their simple and convenient assessment, without the need for electronics and external digital readouts [13,14]. Such sensors have been fabricated using organic fluorophores, such as fluorescein isothiocyanate (FITC) [8] and succinimide substituted 2-(2 -hydroxyphenyl) benzothiazole derivative (BTSA) [15].…”