Recent applications in CEC

Huo, Yuli; Kok, Wim Th.

doi:10.1002/elps.200700639

Cited by 64 publications

(36 citation statements)

References 75 publications

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“…These promising features caused the start of a beautiful collection of CEC applications in all kinds of areas, such as pharmaceutical analysis, chiral analysis and environmental analysis [4]. However, scientists fast encountered the drawbacks associated with this technique.…”

Section: Introductionmentioning

confidence: 99%

Enantioselective capillary electrochromatography: Recent developments and new trends

Mangelings

Heyden

2011

Electrophoresis

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Since its development in the early 1970s, CEC has been studied quite extensively, but unfortunately its use is still mostly located at an academic level. Reasons for this are the limited availability of commercially available stationary phases (SPs) and columns, along with some practical limitations, such as column fragility, lack of column robustness and reproducibility. Nevertheless, CEC maintains a place among the separation techniques, probably because of its unique feature to combine two separation principles. Also in the field of chiral separations, CEC is often used as a separation technique and already showed its potential for this kind of analyses. This overview will focus on the recent applications, i.e. between 2006 and 2010, in enantioselective analysis by means of CEC. For the selected applications, the used SPs (chiral selectors) and their potential for future method development or screening purposes will be evaluated and critically discussed.

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Section: Introductionmentioning

confidence: 99%

Enantioselective capillary electrochromatography: Recent developments and new trends

Mangelings

Heyden

2011

Electrophoresis

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“…CEC is a hybrid separation technique that couples the high separation efficiency of CE with the superior selectivity of HPLC [1]. Despite the fact that CEC is recognized as having tremendous potential in the pharmaceutical and biomedical fields, several technical problems have to be addressed before the technique achieves general acceptance and finds widespread applications.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured pillars based on vertically aligned carbon nanotubes as the stationary phase in micro‐CEC

Yang

Wang

et al. 2009

Electrophoresis

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We present a micro-CEC chip carrying out a highly efficient separation of dsDNA fragments through vertically aligned multi-wall carbon nanotubes (MWCNTs) in a microchannel. The vertically aligned MWCNTs were grown directly in the microchannel to form straight nanopillar arrays as ordered and directional chromatographic supports. 1-Pyrenedodecanoic acid was employed for the surface modification of the MWCNTs' stationary phase to adsorb analytes by hydrophobic interactions. This device was used for separating dsDNA fragments of three different lengths (254, 360, and 572 bp), and fluorescence detection was employed to verify the electrokinetic transport in the MWCNT array. The micro-CEC separation of the three compounds was achieved in less than 300 s at a field strength of 66 V/cm due to superior laminar flow patterns and a lower flow resistance resulting from the vertically aligned MWCNTs being used as the stationary phase medium. In addition, a fivefold reduction of band broadening was obtained when the analyte was separated by the chromatographic MWCNT array channel instead of the CE channel. From all of the results, we suggest that an in situ grown and directional MWCNT array can potentially be useful for preparing more diversified forms of stationary phases for vertically efficient chip-based electrochromatography.

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“…Currently, most of these analyses are performed by HPLC, but the characteristics and availability of CE instrumentation have made CEC a technique with potential in these industries. (Lerma-Garcia et al, 2008;Huo et al, 2008) The parabens are esters of p-hydroxybenzoic acid that differ in the alkyl group linked to the ester functional group. The parabens have antimicrobial functions and thus act as preservatives in food, pharmaceuticals and cosmetics (De Rossi, Desiderio, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Although CEC has been successfully used for the analysis of pharmaceutical compounds (Huo, Kok, 2008;Castro-Puyana et al, 2010), few studies have been performed on the separation of parabens. The work of Huang et al (2004) used monolithic columns based on esters of methacrylates as stationary phase.…”

Section: Introductionmentioning

confidence: 99%

Determination of parabens in sweeteners by capillary electrochromatography

Bottoli

Gutiérrez-Ponce

Aguiar

et al. 2011

Braz. J. Pharm. Sci.

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Parabens, common food preservatives, were analysed by capillary electrochromatography, using a commercial C18 silica (3 µm, 40 cm × 100 µm i. d.) capillary column as separation phase. In order to optimise the separation of these preservatives, the effects of mobile phase composition on the separation were evaluated, as well as the applied voltage and injection conditions. The retention behavior of these analytes was strongly influenced by the level of acetonitrile in the mobile phase. An optimal separation of the parabens was obtained within 18.5 minutes with a pH 8.0 mobile phase composed of 50:50 v/v tris(hydroxymethyl)aminomethane buffer and acetonitrile. The method was successfully applied to the quantitative analysis of paraben preservatives in sweetener samples with direct injection. Uniterms:Capillary eletrochromatography/quantitative analysis. Parabenos/determination. Sweeteners/ quantitative analysis.Os parabenos, empregados como conservantes em alimentos, foram analisados por eletrocromatografia capilar, empregando uma coluna comercial recheada com partículas de sílica-C18 (3 µm, 40 cm × 100 µm d. i.) como fase estacionária de separação. Para otimizar a separação destes conservantes foram avaliados os efeitos da composição da fase móvel na separação, bem como a voltagem e as condições de injeção. O comportamento de retenção dos analitos foi fortemente influenciado pela proporção de acetonitrila na fase móvel. A separação dos parabenos foi alcançada em 18,5 min com uma fase móvel contendo tampão tris(hidroximetil)aminometano e acetonitrila na proporção 50:50 v/v. O método foi aplicado na análise quantitativa de parabenos em adoçantes empregando a injeção direta das amostras.Unitermos: Eletrocromatografia capilar/análise quantitativa. Parabenos/determinação. Adoçantes/ análise quantitativa.

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Recent applications in CEC

Cited by 64 publications

References 75 publications

Enantioselective capillary electrochromatography: Recent developments and new trends

Enantioselective capillary electrochromatography: Recent developments and new trends

Nanostructured pillars based on vertically aligned carbon nanotubes as the stationary phase in micro‐CEC

Determination of parabens in sweeteners by capillary electrochromatography

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