Enantioselective capillary electrochromatography: Recent developments and new trends

Abstract

Since its development in the early 1970s, CEC has been studied quite extensively, but unfortunately its use is still mostly located at an academic level. Reasons for this are the limited availability of commercially available stationary phases (SPs) and columns, along with some practical limitations, such as column fragility, lack of column robustness and reproducibility. Nevertheless, CEC maintains a place among the separation techniques, probably because of its unique feature to combine two separation principles. Also in the field of chiral separations, CEC is often used as a separation technique and already showed its potential for this kind of analyses. This overview will focus on the recent applications, i.e. between 2006 and 2010, in enantioselective analysis by means of CEC. For the selected applications, the used SPs (chiral selectors) and their potential for future method development or screening purposes will be evaluated and critically discussed.

Chiral Separations in Capillary Electrophoresis

“…57,58 When columns are compared in the CEC and nanoliquid chromatography mode using the same mobile phase, superior performance under CEC conditions is often observed (Figure 8). The mobile phase is driven through the column by the EOF generated upon application of the electric field as a consequence of the charged surface of the CSP and/or the capillary.…”

Chiral Separation of Basic Compounds on Sulfated β-Cyclodextrin-Coated Zirconia Monolith by Capillary Electrochromatography

“…10,12,13 Zirconia is a viable alternative to silica as the support, due to its unique and extraordinary chemical, mechanical and thermal stability.…”

Study on the separation of ofloxacin enantiomers by hydroxyl-propyl-β-cyclodextrin as a chiral selector in capillary electrophoresis: a computational approach

“…Customarily, in CE addition of chiral selectors to the background electrolyte (BGE) facilitated resolution of a huge number of chiral compounds. A glance at the literature can easily reveal the fact that macrocycle antibiotics and cyclodextrins are the most dominantly utilized running buffer additives [1,3,[5][6][7][8][9][10][11][12]. So far the general mechanism adopted for the chiral resolution is the one based on the formation of transient diastereoisomers that exhibit different thermodynamic stability with an ensuing difference in migration times.…”