Recent advances of electrochemical and optical biosensors for detection of C-reactive protein as a major inflammatory biomarker

Sohrabi, Hessamaddin; Kordasht, Houman Kholafazad; Pashazadeh-Panahi, Paria; Nezhad‐Mokhtari, Parinaz; Hashemzaei, Mahmoud; Majidi, Mir Reza; Mosafer, Jafar; Oroojalian, Fatemeh; Mokhtarzadeh, Ahad; Guárdia, Miguel de la

doi:10.1016/j.microc.2020.105287

Cited by 74 publications

(32 citation statements)

References 125 publications

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“…Additionally, local surface plasmon resonance (LSPR) has been applied to these sensors [ 97 , 98 ]; in LSPR, vibration is generated on the surface of metal nanoparticles with local surfaces on the SPR sensor [ 99 , 100 ]. Figure 3 A shows the basic principle of LSPR, which can detect biological interactions on the nanoscale due to the bonding between the electromagnetic field (EM) and spatially constrained free electrons.…”

Section: Optical-based Biosensormentioning

confidence: 99%

Recent Advances in CRP Biosensor Based on Electrical, Electrochemical and Optical Methods

Noh

Kim

et al. 2021

Sensors

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C-reactive protein (CRP) is an acute-phase reactive protein that appears in the bloodstream in response to inflammatory cytokines such as interleukin-6 produced by adipocytes and macrophages during the acute phase of the inflammatory/infectious process. CRP measurement is widely used as a representative acute and chronic inflammatory disease marker. With the development of diagnostic techniques measuring CRP more precisely than before, CRP is being used not only as a traditional biomarker but also as a biomarker for various diseases. The existing commercialized CRP assays are dominated by enzyme-linked immunosorbent assay (ELISA). ELISA has high selectivity and sensitivity, but its limitations include requiring complex analytic processes, long analysis times, and professional manpower. To overcome these problems, nanobiotechnology is able to provide alternative diagnostic tools. By introducing the nanobio hybrid material to the CRP biosensors, CRP can be measured more quickly and accurately, and highly sensitive biosensors can be used as portable devices. In this review, we discuss the recent advancements in electrochemical, electricity, and spectroscopy-based CRP biosensors composed of biomaterial and nanomaterial hybrids.

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Section: Optical-based Biosensormentioning

confidence: 99%

Recent Advances in CRP Biosensor Based on Electrical, Electrochemical and Optical Methods

Noh

Kim

et al. 2021

Sensors

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“…The literature search related to NSE, IL-6, IL-8 and CRP biomarkers has been limited to the last three years (from January 2018 up to December 2020), mainly due to a large number of strategies reported in the past decade, as well as already available reviews summarizing EC strategies for the detection of these specic targets (e.g. CRP; [101][102][103] EC (bio)sensor conguration typically comprises two main parts: (i) immobilized recognition element providing the selectivity to the target analyte (T) and (ii) the EC transducer serving as a converter of the biorecognition event to an electronic signal. 107,108 The most common transducers reported for mTBI-related biosensors include gold electrodes (disk, microelectrodes, lms and interdigitated electrodes 109,110 ), carbon-based (glassy carbon, carbon paste, carbon nanotubes (CNTs) 111 ) and graphene-based materials.…”

Section: Design Of Electrochemical Sensors For Detection Of Protein Biomarkers Relevant To Mtbimentioning

confidence: 99%

Electrochemical sensing of blood proteins for mild traumatic brain injury (mTBI) diagnostics and prognostics: towards a point-of-care application

2021

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“…Nowadays, CRP determination is performed by a variety of immunochemical methods [11,12], such as immunoturbidimetric assays [13], enzyme-linked immunosorbent assays [14], chemiluminescence [15] and fluorescence immunoassays [16]. These methods provide high sensitivity and accuracy; however, because they require special equipment and skilled personnel, they are performed mainly at hospitals' central laboratories, which might lead to increased workflow and delays in the delivery of tests results [17,18]. Consequently, the current challenge in diagnostics field is to overcome the limitations of conventional techniques by the development of methods and devices that can provide fast and reliable quantitative results at the Point-of-Care (PoC).…”

Section: Introductionmentioning

confidence: 99%

Development of a Point-of-Care System Based on White Light Reflectance Spectroscopy: Application in CRP Determination

et al. 2021

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The development of methods and miniaturized systems for fast and reliable quantitative determinations at the Point-of-Care is a top challenge and priority in diagnostics. In this work, a compact bench-top system, based on White Light Reflectance Spectroscopy, is introduced and evaluated in an application with high clinical interest, namely the determination of C-Reactive protein (CRP) in human blood samples. The system encompassed all the necessary electronic and optical components for the performance of the assay, while the dedicated software provided the sequence and duration of assay steps, the reagents flow rate, the real-time monitoring of sensor response, and data processing to deliver in short time and accurately the CPR concentration in the sample. The CRP assay included two steps, the first comprising the binding of sample CRP onto the chip immobilized capture antibody and the second the reaction of the surface immunosorbed CRP molecules with the detection antibody. The assay duration was 12 min and the dynamic range was from 0.05 to 200 μg/mL, covering both normal values and acute inflammation incidents. There was an excellent agreement between CRP values determined in human plasma samples using the developed device with those received for the same samples by a standard diagnostic laboratory method.

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Recent advances of electrochemical and optical biosensors for detection of C-reactive protein as a major inflammatory biomarker

Cited by 74 publications

References 125 publications

Recent Advances in CRP Biosensor Based on Electrical, Electrochemical and Optical Methods

Recent Advances in CRP Biosensor Based on Electrical, Electrochemical and Optical Methods

Electrochemical sensing of blood proteins for mild traumatic brain injury (mTBI) diagnostics and prognostics: towards a point-of-care application

Development of a Point-of-Care System Based on White Light Reflectance Spectroscopy: Application in CRP Determination

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