Recent Advances of Carbon Nanotubes‐based Electrochemical Immunosensors for the Detection of Protein Cancer Biomarkers

Feng, Taotao; Wang, Yue; Qiao, Xiuwen

doi:10.1002/elan.201600512

Cited by 38 publications

(17 citation statements)

References 97 publications

(90 reference statements)

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“…In recent years, carbon-based matrices involving graphene oxide (GO) [ 28 ], reduced graphene oxide (rGO) [ 29 ], graphene sheets [ 30 , 31 , 32 ], carbon nanotubes [ 24 , 33 ] and their composites with nanoparticles [ 33 , 34 , 35 ], polymers [ 36 ], etc., have recently attracted much attention owing to their high conductivity, large surface area, and stability. In most of the cases, blocking of free surface areas on the sensor chip after capture molecule binding was achieved using BSA solution incubation.…”

Section: Matrix Selection Modification and Development Of Immunosmentioning

confidence: 99%

“…With the advances in material science and chemistry newer nano and hybrid materials have been explored in recent years to enhance the amplification of the signal. These advanced materials act as carriers for loading of multiple enzyme molecules and thus enhance the signal [ 10 , 24 , 25 ]. However, the use of redox tags and enzyme-mimicking molecules are also receiving much attention in the development of advanced immunosensors.…”

Section: Electrochemical Elisa Based Detectionmentioning

confidence: 99%

“…[ 2 ]. And for sensitive detection researchers have utilized various tags involving redox enzymes, metallic particles, quantum dots, etc., which they have used directly or in combination with another matrix for enhanced loading [ 6 , 14 , 24 , 25 ]. Other than these, the activity and recognition ability of developed sensors also depend on how and where the capturing molecule is immobilized and how well it can interact with the target analyte.…”

Section: Introductionmentioning

confidence: 99%

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Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection

Arya

Estrela

2018

Sensors

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Electrochemical enzyme-linked immunosorbent assay (ELISA)-based immunoassays for cancer biomarker detection have recently attracted much interest owing to their higher sensitivity, amplification of signal, ease of handling, potential for automation and combination with miniaturized analytical systems, low cost and comparative simplicity for mass production. Their developments have considerably improved the sensitivity required for detection of low concentrations of cancer biomarkers present in bodily fluids in the early stages of the disease. Recently, various attempts have been made in their development and several methods and processes have been described for their development, amplification strategies and testing. The present review mainly focuses on the development of ELISA-based electrochemical immunosensors that may be utilized for cancer diagnosis, prognosis and therapy monitoring. Various fabrication methods and signal enhancement strategies utilized during the last few years for the development of ELISA-based electrochemical immunosensors are described.

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Section: Matrix Selection Modification and Development Of Immunosmentioning

confidence: 99%

Section: Electrochemical Elisa Based Detectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection

Arya

Estrela

2018

Sensors

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“…CNTs promote electron transfer and possess high stability, low background noise, rapid electrode kinetics, and excellent biocompatibility. Therefore, CNTs are widely used in various biosensor constructions for preparing the sensing layer of the sensor and for fabricating labels for signal amplification in sandwich-type biosensors [ 19 , 30 , 31 , 32 ].…”

Section: Detection Of Cancer and Disease Biomarkersmentioning

confidence: 99%

Carbon Nanomaterial Based Biosensors for Non-Invasive Detection of Cancer and Disease Biomarkers for Clinical Diagnosis

Pasinszki

Krebsz

Tung

et al. 2017

Sensors

142

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The early diagnosis of diseases, e.g., Parkinson’s and Alzheimer’s disease, diabetes, and various types of cancer, and monitoring the response of patients to the therapy plays a critical role in clinical treatment; therefore, there is an intensive research for the determination of many clinical analytes. In order to achieve point-of-care sensing in clinical practice, sensitive, selective, cost-effective, simple, reliable, and rapid analytical methods are required. Biosensors have become essential tools in biomarker sensing, in which electrode material and architecture play critical roles in achieving sensitive and stable detection. Carbon nanomaterials in the form of particle/dots, tube/wires, and sheets have recently become indispensable elements of biosensor platforms due to their excellent mechanical, electronic, and optical properties. This review summarizes developments in this lucrative field by presenting major biosensor types and variability of sensor platforms in biomedical applications.

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“…The development of novel immuno‐biosensors for highly sensitive, selective, and rapid cancer biomarker detection is of paramount importance for early diagnosis, effective treatment and prognosis of cancer . Carbon‐based nanomaterials, such as: graphene oxide (GO) , carbon nanotubes (CNTs) , mesoporous carbon , carbon quantum dots , etc., have brought many tremendous achievements in immuno‐biosensors.…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanomaterials‐based Electrochemical Immunoassay with β‐Galactosidase as Labels for Carcinoembryonic Antigen

Jin

Lou

et al. 2018

Electroanalysis

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In this study, a novel signal‐amplified strategy for sensitive electrochemical sandwiched immunoassay of carcinoembryonic antigen (CEA) was constructed based on aminofunctionalized graphene oxide (GO‐NH2) supported AgNPs used as catalytic labels of secondary anti‐CEA and β‐galactosidase (β‐Gal), Meanwhile, sulfhydrylation single‐wall carbon nanotubes (SWCNTs‐SH) as substrate materials embellished gold electrode through Au‐SH and connected with gold nanoparticles to form anti‐CEA/AuNPs/SWCNTs‐SH/Au sensing platform through layer‐by‐layer. In the presence of analyte CEA, a sandwich‐type immunoassay format was employed for determination of CEA by using the labeled β‐Gal toward the reduction of p‐aminophenyl galactopyranoside (PAPG) and the redox reaction of AgNPs. Under optimal conditions, the increase in the current was proportional to the concentration of CEA from 0.1 pg/mL to 200 ng/mL. The detection limit (LOD) was 0.036 pg/mL CEA at 3σ. The electrochemical immunoassay displayed an acceptable precision, selectivity, stability. Clinical serum specimens were assayed with the method, and the results were in acceptable agreement with those obtained from the referenced electrochemiluminescent method.

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Recent Advances of Carbon Nanotubes‐based Electrochemical Immunosensors for the Detection of Protein Cancer Biomarkers

Abstract: Electroanalysis 2017,29, 662-675 671 Review Fig. 11. Theillustrations of the single/multiple-HRP-based PSA immunosensor (reprinted with permission from [98]).

Cited by 38 publications

References 97 publications

Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection

Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection

Carbon Nanomaterial Based Biosensors for Non-Invasive Detection of Cancer and Disease Biomarkers for Clinical Diagnosis

Carbon Nanomaterials‐based Electrochemical Immunoassay with β‐Galactosidase as Labels for Carcinoembryonic Antigen

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