Carbon Nanomaterial Based Biosensors for Non-Invasive Detection of Cancer and Disease Biomarkers for Clinical Diagnosis

Pasinszki, Tibor; Krebsz, Melinda; Tung, Thanh; Lošić, Dušan

doi:10.3390/s17081919

Cited by 140 publications

(63 citation statements)

References 159 publications

(183 reference statements)

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“…Several types of nanoparticles, such as quantum dots [ 8 , 9 ], silver and gold nanoparticles [ 10 ], and hydrogels [ 11 ] have been used in applications for diagnosing, monitoring, and treating different diseases. However, these nanoparticles are generally addressed to drug delivery and not to the sequestering or capturing of proteins and peptides.…”

Section: Introductionmentioning

confidence: 99%

Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides

et al. 2017

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Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution of 243.9 ± 11.6 nm and an estimated surface charge of −38.1 ± 0.9 mV. The successful attachment of compounds at every stage of synthesis was evidenced by ATR-FTIR. The capture of model peptides was determined by mass spectrometry, indicating that only the peptide with a long sequence of hydrophobic amino acids (alpha zein 34-mer) interacted with the molecular bait. FCSNP excluded the high molecular weight protein (HMW), BSA, and captured LMW proteins (myoglobin and aprotinin), as evidenced by SDS-PAGE. Functionalization of nanoparticles with Cibacron Blue was crucial to capture these molecules. FCSNP were stable after twelve months of storage and maintained a capacity of 3.1–3.4 µg/mg.

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Section: Introductionmentioning

confidence: 99%

Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides

et al. 2017

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“…To this end, task‐specific protein sensors with a nanoscale sensing mechanism (e.g., nanoparticle‐based biosensors) can offer great potentials. [ 3,4 ] For instance, there has been a great interest in the label‐free optical detection of proteins in purified [ 5 ] and crude biological samples. [ 6 ] Amongst various techniques explored for this purpose, bio‐optical sensors based on peptide‐decorated nanostructures have attracted significant attention because of their immanent low toxicity and high translational values.…”

Section: Figurementioning

confidence: 99%

Nanoassembled Peptide Biosensors for Rapid Detection of Matrilysin Cancer Biomarker

Behi

Naficy

Chandrawati

et al. 2020

Small

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high translational values. [7][8][9] Commonly, organic fluorophore dyes and quantum dots (QDs) have been employed in order to modify peptides such as Escherichia coli for fluorescent imaging and biosensing. [10,11] Still, fluorescent dyes have several issues such as susceptibility to photobleaching and slender specificity for bioconjugation to particular reactive sites. [12,13] Most QDs, on the other hand, suffer from cytotoxicity for biomedical applications. [14][15][16] Carbon quantum dots (CDs), [17] a class of 0D carbon nanomaterials (1-10 nm) with unique optical properties, high photo stability, excellent biocompatibility, and facile preparation are promising substitutes to fluorescent dyes and QDs. [18,19] In addition, gold nanoparticles (AuNPs) have shown remarkable physical and optical properties in nanomedicine applications, specifically in the field of nanobiosensors. [20][21][22] The chemical and physical interactions between AuNPs and proteins or peptides have already been characterized with biophysical, [23] biochemical, [24] and computational methods, [25] which indicate the diversity of AuNPs as an ideal model material for developing new peptide/protein optical biosensors. So far, several studies have been conducted aiming at manipulating peptide self-nanoassembly into desired nanoarchitectures for sensing applications. [26][27][28][29][30][31] For instance, peptide-functionalized AuNPs with tunable, reversible, [32] and irreversible [33,34] aggregations were synthesized as nanobiosensors to detect phospholipases and proteolytic activities, respectively. Yet, it remains a challenge to fully engineer the nanoarchitecture of peptide and hybrid nanoparticles to respond to minuscule concentrations of target analytes (e.g., cancer biomarker) in a short amount of time.In the light of the necessity for rapid detection of cancerrelated protein biomarkers, we have engineered a sensitive and selective fluorescent, label-free protein detection platform hereafter referred to as the PACD (peptide-Au-carbon dots) nanoprobe (Sections S1-S7, Supporting Information). We based this class of hybrid nanobiosensing platform on a synthetic polypeptide aptamer sensitive to the target biomarker which is anchored to AuNPs from one end and semiconducting zero dimensional CDs from the other end with niche electro-optical properties (Figure 1a). The polypeptide, referred to as the bioreceptor interchangeably, belongs to a family of helix-loophelix polypeptides de novo (JR2EC). [35] The bioreceptors and the surface-functionalized CDs were assembled step-wise on the surface of AuNPs, forming a stable nanoprobe (Figure 1b). As the active component of the nanobiosensors, JR2ECEarly detection of cancer is likely to be one of the most effective means of reducing the cancer mortality rate. Hence, simple and ultra-quick methods for noninvasive detection of early-stage tumors are highly sought-after. In this study, a nanobiosensing platform with a rapid response time of nearly 30 s is introduced for the detection of matrilysin-the sal...

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“…Fibrous nanomaterials are important for many potential applications including: wound dressing (Hassiba et al ., ); filtration, including air filtration (Al‐Attabi et al ., ); biosensors (Pasinszki et al ., ); tissue engineering such as skin tissue engineering (Jiang et al ., ) and targeted drug delivery to, e.g. bone (Cheng et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Automatic diameter and orientation distribution determination of fibrous materials in micro X‐ray CT imaging data

Chiverton

Kao

Roldo

et al. 2018

Journal of Microscopy

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Fibrous nanomaterials such as electrospun materials have many uses ranging from tissue engineering to biosensors. High-resolution imaging is an important component in the characterization of these materials. Important parameters required to predict and study the properties of fibre rich materials include diameter and orientation distribution as well as fibre spacing. The orientations and the relative dimensions of the fibres can be measured via specially designed imaging software. Difficulties in this measurement process can arise if fibres are distributed in close proximity to each other in relation to the resolution of the imaging modality. For example, if some automation is required in the measurement process and, particularly, if the automated processes are not designed for situations where the fibres are in close proximity to each other. This work is therefore concerned with the development of automated measurement techniques to provide estimates of the diameters of fibres and also the orientation distribution. The software automatically detects special points in the fibrous materials where fibres can be considered to have some delineation from surrounding fibres. These sparse points are considered to be points at which estimates of the fibres' properties can be quantified. Aligned and randomly distributed electrospun poly(caprolactone) nanofibres were prepared. Imaging of these materials was performed with an X-ray Computer Tomography system with an image voxel size of 0.15 × 0.15 × 0.15 μm . Scanning Electron Microscopy images were also obtained. Fibre diameters estimated using images from both modalities using the developed techniques were found to be in agreement. Orientation distribution was summarized with multiscale entropy and found to be consistent with visual observation across different scales. LAY DESCRIPTION: Fibres are present in many types of materials which can include, for some materials, very small fibres e.g. a few nanometres in diameter. Very small fibres are present in collagen and elastin which are common tissues of many organs in many types of living things. The sizes of these very small fibres and how they are arranged are important information that can help in the understanding of the overall properties of these materials. Materials with very small fibres can also be synthesized using specialised techniques. The properties of these synthesized fibrous materials are also important to help in understanding how the materials will perform in various different applications. Applications are many and can range from tissue engineering to drug delivery. Some properties of these materials can be shown, visually, with the aid of 3D imaging techniques such as X-ray Computer Tomography (XCT) or in 2D, with Scanning Electron Microscopy (SEM) but at a higher magnification. The work described here is centred around the development of computer algorithms to automatically determine material properties from 3D XCT images. Tests are performed with material samples, where the fibres are aligned (in semi...

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Carbon Nanomaterial Based Biosensors for Non-Invasive Detection of Cancer and Disease Biomarkers for Clinical Diagnosis

Cited by 140 publications

References 159 publications

Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides

Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides

Nanoassembled Peptide Biosensors for Rapid Detection of Matrilysin Cancer Biomarker

Automatic diameter and orientation distribution determination of fibrous materials in micro X‐ray CT imaging data

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