Recent advances in the production of value added chemicals and lipids utilizing biodiesel industry generated crude glycerol as a substrate – Metabolic aspects, challenges and possibilities: An overview

Narisetty, Vivek; Sindhu, Raveendran; Madhavan, Aravind; Anju, Alphonsa Jose; Castro, Eulógio; Faraco, Vincenza; Pandey, Ashok; Binod, Parameswaran

doi:10.1016/j.biortech.2017.05.056

Cited by 126 publications

(61 citation statements)

References 101 publications

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“…Y. lipolytica is a fascinating non‐conventional yeast that can be often found in dairy products such as raw milk, yogurt, cheese and sausage . It not only enables to tolerate a fairly wide range of the environment from hypersaline concentration to a wide acidity range from pH 3 to 8 , but also enables to utilize the various substrates as sole carbon source from sugars, glycerols, alkanes, peptone, fatty acids to other types of lipids .…”

Section: Assimilations Of Carbon Sourcesmentioning

confidence: 99%

Cellular and metabolic engineering of oleaginous yeast Yarrowia lipolytica for bioconversion of hydrophobic substrates into high‐value products

Soong

Liu

Yoon

et al. 2019

Engineering in Life Sciences

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The non‐conventional oleaginous yeast Yarrowia lipolytica is able to utilize both hydrophilic and hydrophobic carbon sources as substrates and convert them into value‐added bioproducts such as organic acids, extracellular proteins, wax esters, long‐chain diacids, fatty acid ethyl esters, carotenoids and omega‐3 fatty acids. Metabolic pathway analysis and previous research results show that hydrophobic substrates are potentially more preferred by Y. lipolytica than hydrophilic substrates to make high‐value products at higher productivity, titer, rate, and yield. Hence, Y. lipolytica is becoming an efficient and promising biomanufacturing platform due to its capabilities in biosynthesis of extracellular lipases and directly converting the extracellular triacylglycerol oils and fats into high‐value products. It is believed that the cell size and morphology of the Y. lipolytica is related to the cell growth, nutrient uptake, and product formation. Dimorphic Y. lipolytica demonstrates the yeast‐to‐hypha transition in response to the extracellular environments and genetic background. Yeast‐to‐hyphal transition regulating genes, such as YlBEM1, YlMHY1 and YlZNC1 and so forth, have been identified to involve as major transcriptional factors that control morphology transition in Y. lipolytica. The connection of the cell polarization including cell cycle and the dimorphic transition with the cell size and morphology in Y. lipolytica adapting to new growth are reviewed and discussed. This review also summarizes the general and advanced genetic tools that are used to build a Y. lipolytica biomanufacturing platform.

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Section: Assimilations Of Carbon Sourcesmentioning

confidence: 99%

Cellular and metabolic engineering of oleaginous yeast Yarrowia lipolytica for bioconversion of hydrophobic substrates into high‐value products

Soong

Liu

Yoon

et al. 2019

Engineering in Life Sciences

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“…As previously mentioned, glycerol is a byproduct in the biodiesel industry. However, biodiesel-derived glycerol (bio-glycerol) cannot satisfy purity and cost requirement [13]. For efficient and economical utilization of bio-glycerol, it needs to be converted to value-added chemicals, such as glycerol carbonate [78][79][80][81].…”

Section: Chemical Process For Dmc-based Chemicals Productionmentioning

confidence: 99%

“…With a growing biodiesel industry, a large surplus of glycerol has been produced [10,11]. For example, over 1400 kilo tons of glycerol were created from biodiesel production Glycerol, as a low-cost feedstock, can be used for chemical, pharmaceutical, and cosmetic industries, but the cost of separation and purification of crude glycerol is relatively high [10,[13][14][15]. To overcome the issues of alcohol-mediated transesterification, an alternative novel acyl acceptor, dimethyl carbonate (DMC), has been employed for glycerol-free biodiesel synthesis.…”

Section: Introductionmentioning

confidence: 99%

Environmentally-Benign Dimethyl Carbonate-Mediated Production of Chemicals and Biofuels from Renewable Bio-Oil

Kim

Lee

2017

Energies

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Due to the increasing emission of carbon dioxide (CO 2 ), the development of fuels and chemicals based on renewable resources has attracted much attention. Bio-oil, as a carbon rich material, has been considered as a feedstock for biodiesel production. In conventional methanol-mediated transesterification of bio-oil for biodiesel production, significant amounts of glycerol are being generated as a byproduct. In order to overcome these issues, dimethyl carbonate (DMC) has been recently used as an alternative acyl acceptor to avoid the generation of glycerol. DMC is an environmentally-benign chemical reagent and reactive solvent due to safety, health, and environmental benefits. Moreover, DMC can be produced from CO 2 . Co-production of biodiesel and chemicals such as glycerol carbonate is possible as the concept of zero-waste utilization of bio-oil. Value-added chemicals can be synthesized using DMC as a reagent. This paper provides a review on the physical and chemical properties of DMC as a solvent, as well as the production methods for DMC. DMC-mediated production of various chemicals and fuels in both chemical and enzymatic processes are discussed together with their pros and cons.

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“…Glycerol is an essential carbon source for the production of 1,3-PDO from lactic acid bacteria. Crude glycerol is a by-product of the biodiesel industry, and about 10% (w/w) glycerol is produced during biodiesel production [19]. The glycerol produced from a biodiesel plant is usually 40-70% (w/w) before acid treatment, and 80% (w/w) after acid treatment [20].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

Wang

Heo

et al. 2020

Microb Cell Fact

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Background: 1,3-propanediol (1,3-PDO) is the most widely studied value-added product that can be produced by feeding glycerol to bacteria, including Lactobacillus sp. However, previous research reported that L. reuteri only produced small amounts and had low productivity of 1,3-PDO. It is urgent to develop procedures that improve the production and productivity of 1,3-PDO. Results:We identified a novel L. reuteri CH53 isolate that efficiently converted glycerol into 1,3-PDO, and performed batch co-fermentation with glycerol and glucose to evaluate its production of 1,3-PDO and other products. We optimized the fermentation conditions and nitrogen sources to increase the productivity. Fed-batch fermentation using corn steep liquor (CSL) as a replacement for beef extract led to 1,3-PDO production (68.32 ± 0.84 g/L) and productivity (1.27 ± 0.02 g/L/h) at optimized conditions (unaerated and 100 rpm). When CSL was used as an alternative nitrogen source, the activity of the vitamin B12-dependent glycerol dehydratase (dhaB) and 1,3-propanediol oxidoreductase (dhaT) increased. Also, the productivity and yield of 1,3-PDO increased as well. These results showed the highest productivity in Lactobacillus species. In addition, hurdle to 1,3-PDO production in this strain were identified via analysis of the half-maximal inhibitory concentration for growth (IC50) of numerous substrates and metabolites. Conclusions:We used CSL as a low-cost nitrogen source to replace beef extract for 1,3-PDO production in L. reuteri CH53. These cells efficiently utilized crude glycerol and CSL to produce 1,3-PDO. This strain has great promise for the production of 1,3-PDO because it is generally recognized as safe (GRAS) and non-pathogenic. Also, this strain has high productivity and high conversion yield.

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Recent advances in the production of value added chemicals and lipids utilizing biodiesel industry generated crude glycerol as a substrate – Metabolic aspects, challenges and possibilities: An overview

Cited by 126 publications

References 101 publications

Cellular and metabolic engineering of oleaginous yeast Yarrowia lipolytica for bioconversion of hydrophobic substrates into high‐value products

Cellular and metabolic engineering of oleaginous yeast Yarrowia lipolytica for bioconversion of hydrophobic substrates into high‐value products

Environmentally-Benign Dimethyl Carbonate-Mediated Production of Chemicals and Biofuels from Renewable Bio-Oil

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

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