Recent advances in production of lignocellulolytic enzymes by solid-state fermentation of agro-industrial wastes

Leite, Paulina; Sousa, Daniel; Fernandes, Helena; Ferreira, Miguel; Costa, Ana Rita; Filipe, Diogo; Gonçalves, Marisa Pereira; Perés, Helena; Belo, Isabel; Salgado, José Manuel

doi:10.1016/j.cogsc.2020.100407

Cited by 71 publications

(21 citation statements)

References 46 publications

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“…Solid-state fermentation (SSF) is a white biotechnological process that may be used to obtain carbohydrases through the hydrolysis of adequate solid substrates by selected microorganisms. SSF differs from submerged fermentation (SmF) as it occurs with little or no free water, is suitable for filamentous fungi growth, and is a cost-effective and eco-friendly process 12 . SmF is a liquid-state fermentation, commonly uses bacteria and allows easy final product recovery through filtration or centrifugation 13 .…”

Section: Introductionmentioning

confidence: 99%

Solid-state fermented brewer's spent grain enzymatic extract increases in vitro and in vivo feed digestibility in European seabass

Fernandes

Moyano

Castro

et al. 2021

Sci Rep

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Brewer’s spent grain (BSG) is the largest by-product originated from the brewery industry with a high potential for producing carbohydrases by solid-state fermentation. This work aimed to test the efficacy of a carbohydrases-rich extract produced from solid-state fermentation of BSG, to enhance the digestibility of a plant-based diet for European seabass (Dicentrarchus labrax). First, BSG was fermented with A. ibericus to obtain an aqueous lyophilized extract (SSF-BSG extract) and incorporated in a plant-based diet at increasing levels (0—control; 0.1%, 0.2%, and 0.4%). Another diet incorporating a commercial carbohydrases-complex (0.04%; Natugrain; BASF) was formulated. Then, all diets were tested in in vitro and in vivo digestibility assays. In vitro assays, simulating stomach and intestine digestion in European seabass, assessed dietary phosphorus, phytate phosphorus, carbohydrates, and protein hydrolysis, as well as interactive effects between fish enzymes and dietary SSF-BSG extract. After, an in vivo assay was carried out with European seabass juveniles fed selected diets (0—control; 0.1%, and 0.4%). In vitro digestibility assays showed that pentoses release increased 45% with 0.4% SSF-BSG extract and 25% with Natugrain supplemented diets, while amino acids release was not affected. A negative interaction between endogenous fish enzymes and SSF-BSG extract was observed in both diets. The in vivo digestibility assay corroborated in vitro data. Accordingly, the dietary supplementation with 0.4% SSF-BSG increased the digestibility of dry matter, starch, cellulose, glucans, and energy and did not affect protein digestibility. The present work showed the high potential of BSG to produce an added-value functional supplement with high carbohydrases activity and its potential contribution to the circular economy by improving the nutritional value of low-cost and sustainable ingredients that can be included in aquafeeds.

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Section: Introductionmentioning

confidence: 99%

Solid-state fermented brewer's spent grain enzymatic extract increases in vitro and in vivo feed digestibility in European seabass

Fernandes

Moyano

Castro

et al. 2021

Sci Rep

Self Cite

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“…Moreover, wheat straw is the cellulose-rich substrate, which would allow the efficient endocellulases production. The lignocellulolytic enzymes produced by SSF showed high thermal stability and resistance to alkaline or acidic conditions and thus suitable for industrial applications [32]. In brief, the studied agro-industrial waste showed the feasibility as a solid substrate in sustainable production of low-cost lignocellulolytic enzymes by fungus and thus can have an alternative to commercial substrates.…”

Section: Production Of Lignocellulolytic Enzymesmentioning

confidence: 95%

Sustainable Production of Lignocellulolytic Enzymes in Solid-State Fermentation of Agro-Industrial Waste: Application in Pumpkin (Cucurbita maxima) Juice Clarification

et al. 2021

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Valorization of agro-industrial waste through greener and biotechnological processes are promising approaches to minimize the generation of agro-industrial waste. Therefore, the study aimed to produce lignocellulolytic enzymes from agro-industrial waste under solid-state fermentation (SSF) conditions and study their application in the clarification of pumpkin juice. The SSF was performed with three different combinations of wheat bran + rice bran (WBRB), wheat bran + wheat straw (WBWS), and rice bran + wheat straw (RBWS) as dry solid substrates (1:1) using Fusarium oxysporum (MTCC 7229). The protein, carboxymethyl cellulase (CMCase), and xylanase contents ranged from 0.98–3.90 mg/g, 5.89–6.84 U/g substrate, and 10.08–13.77 U/g substrate, respectively in different agro-industrial waste as substrates (WBRB, WBWS, RBWS, and control). The increase in enzyme concentration (0.50–2.40%) added to pumpkin juice exhibited an increased juice yield (16.30–55.60%), reduced browning index (1.03–0.70), and an increase in clarity (5.31–13.77 %T), which was further confirmed by a total variance of 84.83% by principal component analysis. Thus, the low-cost lignocellulolytic enzymes can be produced from agro-industrial waste that might have applications in food and beverage industries. Hence, this approach could be used as a long-term sustainable and circular source to valorize agro-industrial waste towards the greener future and the preservation of ecosystems.

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“…O descarte desses materiais é de grande importância e, quando realizado de forma inadequada, pode gerar impactos ambientais. Neste sentido, a biotecnologia pode ser uma excelente ferramenta para transformar os resíduos e subprodutos agroindustriais em matérias-primas para a produção de produtos de valor agregado (Leite et al, 2020).…”

Section: Processo De Produçãounclassified

Produção Microbiológica De Enzimas: Uma Revisão/Microbiological Production of Enzymes: A Review

Rigo

Gayeski

Tres

et al. 2021

BJD

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A produção de enzimas a partir de microrganismos possui extensa aplicação na indústria alimentícia buscando a melhoria do processo de produção e outros componentes relacionados. As lipases produzidas por microrganismos têm ganhado um enorme interesse industrial devido à sua versatilidade, maior rendimento e produção independente da estação do ano. O objetivo deste trabalho foi realizar uma revisão bibliográfica da produção microbiológica de enzimas, com foco principalmente em lipases devido a sua importância no meio industrial, identificando quais os principais microrganismos empregados, forma de produção, métodos de isolamento, purificação e aplicação. O trabalho foi desenvolvido por meio de pesquisa bibliográfica realizada em artigos científicos, dissertações e teses localizados nas bases de dados online e portais de pesquisa. Este estudo mostrou que a produção de enzimas por microrganismos vem sendo muito estudada indicando diferentes posteriores aplicações para a indústria. A possibilidade de utilização de resíduos agroindustriais para a produção microbiana de enzimas também é uma boa alternativa para sanar problemas ambientais, além de envolver baixos custos no processo. Para a produção de lipases, existem inúmeros microrganismos que podem atender esta procura, no entanto, os fungos se destacam, sendo os maiores produtores de lipase microbiana.

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Recent advances in production of lignocellulolytic enzymes by solid-state fermentation of agro-industrial wastes

Cited by 71 publications

References 46 publications

Solid-state fermented brewer's spent grain enzymatic extract increases in vitro and in vivo feed digestibility in European seabass

Solid-state fermented brewer's spent grain enzymatic extract increases in vitro and in vivo feed digestibility in European seabass

Sustainable Production of Lignocellulolytic Enzymes in Solid-State Fermentation of Agro-Industrial Waste: Application in Pumpkin (Cucurbita maxima) Juice Clarification

Produção Microbiológica De Enzimas: Uma Revisão/Microbiological Production of Enzymes: A Review

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