Recent advances in micellar electrokinetic chromatography

Pappas, Theron J.; Gayton-Ely, Melissa; Holland, Lisa

doi:10.1002/elps.200410191

Cited by 78 publications

(58 citation statements)

References 190 publications

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“…Capillary EKC (CEKC), namely its micellar mode (CMEKC), last year celebrating 20 years of its invention by Terabe et al [135] and on this occasion reviewed by him [136] and other authors [137,138]. CMEKC is mostly used with ionogenic detergents, anionic SDS, cationic CTAB, or zwitterionic CHAPS; it is suitable for separation of electroneutral peptides, i.e., peptides with blocked or derivatized N-and C-termini and other ionogenic groups of peptide chain, and/or for separation of peptides having the same or very similar charge-to-mass ratio but differing in their hydrophobicity.…”

Section: Electrokinetic Chromatographymentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Electrokinetic Chromatographymentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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“…This is not easily achievable in HPLC. Several workers have demonstrated the ease and flexibility of the MEKC for analysis of neutral and weakly charged compounds [1][2][3][4]. Here, we demonstrate how direct sample injection without any treatment can be applied to the routine analysis of iohexol in serum for assessing the glomerular filtration rate (GFR) and with very good daily reproducibility.…”

Section: Introductionmentioning

confidence: 97%

Serum iohexol analysis by micellar electrokinetic capillary chromatography

Shihabi

Hinsdale

2006

Electrophoresis

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A simple and rapid ( approximately 4 min) method for the measurement of iohexol in serum for assessing the glomerular filtration rate is described. It is based on direct serum injection on the capillary by MEKC. The method is linear between 8 and 260 mg/L, with an RSD of peak height of 2.9%. Several simple steps have contributed to an improved daily precision, such as choosing a high pH buffer, increasing the SDS concentration, frequent standardization, and eliminating any sample pretreatment.

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“…Fluorescence spectrophotometer (FS) and flow cytometry [14,15] [16,17], which has been applied in bioanalysis [18] and clinical analysis [19] in the past decades. However, the quantitative determination of fluorescent compounds physically adsorbed on CNTs is still the problem due to the fluorescence quenching phenomenon of fluorophores on CNTs [20,21].…”

Section: Introductionmentioning

confidence: 99%

MEKC‐LIF analysis of rhodamine123 delivered by carbon nanotubes in K562 cells

et al. 2009

Electrophoresis

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Oxidized single-walled carbon nanotubes (o-SWNTs) were employed as the drug carriers to deliver the small molecules of Rhodamine123 (Rho123) into the K562 cells via physical adsorption. However, due to the fluorescence quenching of Rho123 on carbon nanotubes, the quantitative determination of Rho123 in cells is difficult. Based on the MEKC coupled with LIF detection, a quantitative approach was developed for the determination of Rho123 delivered into K562 cells by o-SWNTs. Where the adsorbed Rho123 on o-SWNTs could be desorbed by SDS in running buffer and be simultaneously separated with o-SWNTs due to the differences of their electrophoretic mobility by applying the electric potential at the both ends of capillary. Using this approach, the intracellular uptakes of Rho123 in multidrug-resistant and multidrug-sensitive leukemia cells were quantified, and the results showed that o-SWNTs could be used as the potential drug carriers to deliver small molecules into cells via the physical adsorption along with the circumventing of multidrug resistance of leukemia cells.

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Recent advances in micellar electrokinetic chromatography

Cited by 78 publications

References 190 publications

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Serum iohexol analysis by micellar electrokinetic capillary chromatography

MEKC‐LIF analysis of rhodamine123 delivered by carbon nanotubes in K562 cells

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