MEKC‐LIF analysis of rhodamine123 delivered by carbon nanotubes in K562 cells

Abstract

Oxidized single-walled carbon nanotubes (o-SWNTs) were employed as the drug carriers to deliver the small molecules of Rhodamine123 (Rho123) into the K562 cells via physical adsorption. However, due to the fluorescence quenching of Rho123 on carbon nanotubes, the quantitative determination of Rho123 in cells is difficult. Based on the MEKC coupled with LIF detection, a quantitative approach was developed for the determination of Rho123 delivered into K562 cells by o-SWNTs. Where the adsorbed Rho123 on o-SWNTs could be desorbed by SDS in running buffer and be simultaneously separated with o-SWNTs due to the differences of their electrophoretic mobility by applying the electric potential at the both ends of capillary. Using this approach, the intracellular uptakes of Rho123 in multidrug-resistant and multidrug-sensitive leukemia cells were quantified, and the results showed that o-SWNTs could be used as the potential drug carriers to deliver small molecules into cells via the physical adsorption along with the circumventing of multidrug resistance of leukemia cells.

Pluronic F108 Coating Decreases the Lung Fibrosis Potential of Multiwall Carbon Nanotubes by Reducing Lysosomal Injury

“…41 This reaction reflects the excitation of ground-state electrons in FITC to the highest occupied molecular orbital, following which electron transfer to the CNT conduction band and then to the ground state leads to energy release in the form of thermal radiation. 39 Quantification of the fluorophore quenching is an indirect measure of the available tube surface for FITC binding. 42 Thus, the degree of quenching could be used to provide a comparison between different coated tubes, but should not be interpreted as accurate measurement of the total surface area (which changes in response to tube agglomeration).…”

In vivo detection of magnetic labeled oxidized multi-walled carbon nanotubes by magnetic resonance imaging

“…Synthesis and characterization of SPIO o-MWCNTs Figure 1 showed the scheme to synthesize (SPIO) o-MWCNTs. Briefly, after oxidization by a mixed acids of HNO 3 and H 2 SO 4 [26], the resulting o-MWCNTs were dispersed in FeCl 3 aqueous solution to load Fe 3+ . The Fe 3+ /o-MWCNTs composites were then filtered out and re-dissolved in 50 mL EG solution with sodium acetate.…”

Cellular Interactions of a Water‐Soluble Supramolecular Polymer Complex of Carbon Nanotubes with Human Epithelial Colorectal Adenocarcinoma Cells

“…Recently, SWNTs have been shown to be promising drug carriers, especially for multidrug‐resistant cells. In one model, Rho 123‐adsorbed SWNTs were shown to circumvent the multidrug resistance of leukemia cells due to the promising ability of SWNTs to act as intracellular carriers with a relatively large capacity and prolonged release ability 27. Moreover, anti‐P‐gp‐functionalized water‐soluble SWNTs show 23‐fold higher binding affinity for drug‐resistant leukemia cells than for drug‐sensitive ones 28.…”