Recent advances in lipidomics for disease research

Yang, Li; Li, Min; Shan, Yabing; Shen, Sensen; Bai, Yu; Liu, Huwei

doi:10.1002/jssc.201500899

Cited by 82 publications

(65 citation statements)

References 157 publications

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“…19, 20 Indeed, recent lipidomics studies revealed a dynamic alteration of the membrane lipid composition in human cancer tissues, 21 and distinct lipid profiles correlated with tumor grade. 22 Moreover, combination of molecular biology and lipidomics techniques revealed some of the mechanisms underlying deregulated lipid metabolism in cancer.…”

Section: Introductionmentioning

confidence: 99%

A distinct function of the retinoblastoma protein in the control of lipid composition identified by lipidomic profiling

et al. 2017

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Here, by combining lipidomics with transcriptome analysis, we demonstrate that Rb depletion in mouse embryonic fibroblastss induces significant alterations in their lipid composition. We discovered that Rb depletion induced increase in lysophosphatidylserine, diacylglycerol (DAG), fatty acid (FA), acylcarnitine, phosphatidylcholine (PC), arachidonoyl ethanolamine, and decrease in phosphatidylglycerol, monoacylglycerol, without change in total lipid per protein levels. Analysis of the acyl chain composition of DAG, PC and phosphatidylserine revealed increase of saturated and mono-unsaturated acyl chains with specific carbon chain length. Consistently, we observed that Rb depletion increased the levels of fatty acids with the corresponding carbon chain length and number of carbon–carbon double bondssuch as myristic acid (14:0), palmitic acid (16:0), stearic acid (18:0) and all forms of FA 18:1. Microarray analysis revealed that Rb depletion induced significant upregulation of enzymes involved in elongation and desaturation of fatty acids. Among these, we found that elongation of long chain fatty acid family member 6 (Elovl6) and stearoyl-CoA desaturase 1 (Scd1) are the most robustly controlled by Rb possibly through E2F and sterol regulatory element-binding protein transcription factors. Depletion of Elovl6 or Scd1 significantly suppressed colony formation, sphere formation and xenograft tumor growth of Rb-deficient tumor cells. Suppression of self-renewal by the SCD1 inhibitor was rescued upon supplementation of the mono-unsaturated fatty acids generated by this enzyme. This study suggests a novel role for Rb in suppressing the malignant progression of tumors by controlling the lipid composition.

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Section: Introductionmentioning

confidence: 99%

A distinct function of the retinoblastoma protein in the control of lipid composition identified by lipidomic profiling

et al. 2017

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“…atherosclerosis), neurodegenerative disease (e.g. Alzheimer’s disease) and infection disease, have been well summarized in other reviews[3,5–7]. In the past few years, the application of lipidomics to studies of skin biology and inflammatory skin diseases has drawn particular attention.…”

Section: Introductionmentioning

confidence: 99%

Lipidomic analysis of epidermal lipids: a tool to predict progression of inflammatory skin disease in humans

Ganguli‐Indra

Indra

2016

Expert Review of Proteomics

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Introduction Lipidomics is the large-scale profiling and characterization of lipid species in a biological system using mass spectrometry. The skin barrier is mainly comprised of corneocytes and a lipid-enriched extracellular matrix. The major skin lipids are ceramides, cholesterol and free fatty acids. Lipid compositions are altered in inflammatory skin disorders with disrupted skin barrier such as atopic dermatitis (AD). Areas covered Here we discuss some of the recent applications of lipidomics in human skin biology and in inflammatory skin diseases such as AD, psoriasis and Netherton syndrome. We also review applications of lipidomics in human skin equivalent and in pre-clinical animal models of skin diseases to gain insight into the pathogenesis of the skin disease. Expert commentary Skin lipidomics analysis could be a fast, reliable and noninvasive tool to characterize the skin lipid profile and to monitor the progression of inflammatory skin diseases such as AD.

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“…Lipidomics is an important branch of metabolomics, which has been extensively introduced to many diseases research, including diabetes mellitus and atherosclerosis [6]. As lipids can be divided into eight categories (fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterol lipids, prenolipids, saccharolipids, and polyketides) containing distinct classes and subclasses [7], and each class of lipids contains a series of molecules with different combination of acyl chains, the diversity of lipids presents a challenge in lipidomics analysis [8].…”

Section: Introductionmentioning

confidence: 99%

Sphingolipids Profiling of Plasma in Patients with Diabetes Mellitus Associated with Atherosclerosis by a Novel Normal-Phase UPLC-QToF MS Method

Yang

Huang

et al. 2017

J. Anal. Test.

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A novel normal-phase UPLC-QToF MS method was developed for sphingolipids analysis, which was then applied to separate and identify seven classes of sphingolipids in plasma. The time-consuming of this novel method was much shorter than previous 2D LC-MS method, while the sensitivity, repeatability and resolution for sphingolipids analysis were better or comparable. Besides, the differences of lipids in human plasma among diabetes mellitus patients, diabetes mellitus associated with atherosclerosis patients and control subjects were also compared. Finally, 12 sphingolipids species were identified as the amounts of which in diabetes mellitus associated with atherosclerosis patients were significantly higher than them in diabetes mellitus patients (VIP [1.0, p value \0.05, fold change [1.5). As the isomers of GalCs and GluCs were successfully separated, it was found that five GluCs, rather than GalCs, were included in these 12 potential biomarkers. These results suggest the need to separate GalCs from GluCs, and also indicated that there may be some extra activation pathways of diabetes mellitus associated with atherosclerosis, different from the atherosclerosis pathway and the diabetes mellitus pathway, causing cardiovascular disease.

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Recent advances in lipidomics for disease research

Cited by 82 publications

References 157 publications

A distinct function of the retinoblastoma protein in the control of lipid composition identified by lipidomic profiling

A distinct function of the retinoblastoma protein in the control of lipid composition identified by lipidomic profiling

Lipidomic analysis of epidermal lipids: a tool to predict progression of inflammatory skin disease in humans

Sphingolipids Profiling of Plasma in Patients with Diabetes Mellitus Associated with Atherosclerosis by a Novel Normal-Phase UPLC-QToF MS Method

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