Sphingolipids Profiling of Plasma in Patients with Diabetes Mellitus Associated with Atherosclerosis by a Novel Normal-Phase UPLC-QToF MS Method

Abstract

A novel normal-phase UPLC-QToF MS method was developed for sphingolipids analysis, which was then applied to separate and identify seven classes of sphingolipids in plasma. The time-consuming of this novel method was much shorter than previous 2D LC-MS method, while the sensitivity, repeatability and resolution for sphingolipids analysis were better or comparable. Besides, the differences of lipids in human plasma among diabetes mellitus patients, diabetes mellitus associated with atherosclerosis patients and control subjects were also compared. Finally, 12 sphingolipids species were identified as the amounts of which in diabetes mellitus associated with atherosclerosis patients were significantly higher than them in diabetes mellitus patients (VIP [1.0, p value \0.05, fold change [1.5). As the isomers of GalCs and GluCs were successfully separated, it was found that five GluCs, rather than GalCs, were included in these 12 potential biomarkers. These results suggest the need to separate GalCs from GluCs, and also indicated that there may be some extra activation pathways of diabetes mellitus associated with atherosclerosis, different from the atherosclerosis pathway and the diabetes mellitus pathway, causing cardiovascular disease.

Exploring potential antidiabetic and anti-inflammatory flavonoids from Euphorbia humifusa with an integrated strategy

“…Diabetes mellitus (DM) is a chronic metabolic disease characterized by reduced insulin sensitivity or insulin deficiency, causing blood hyperglycemia in the postprandial and fasting state ( Kazeem et al, 2021 ). By 2045, it is projected that around 800 million of the global population will be hurt by this pandemic metabolic disorder with 6.7 million recorded deaths in 2021 ( Li et al, 2017 ; Ni et al, 2022 ). Persistent hyperglycemia encompasses multiple complications leading to nephropathy, retinopathy, neuropathy and vascular damage ( Yen et al, 2021 ).…”

Sphingolipidomic study of davidiin-treated HepG2 human hepatocellular carcinoma cells using UHPLC-MS

“…12 LC-MS strategy has been widely employed in sphingolipidomic studies for the discovery of biomarkers of various diseases, such as cancers, [13][14][15] diabetes and atherosclerosis. 16 The LC-MS based sphingolipidomic approach has also been extensively utilized in the evaluation of therapeutic effect of drug and exploration of drug target. [17][18][19] Davidiin is an ellagitannin originating from Polygonum capitatum (P. capitatum).…”