Recent advances in enzyme immobilization based on nanoflowers

Role of pretty nanoflowers as novel versatile analytical tools for sensing in biomedical and bioanalytical applications

“…At the final step, the growth of the hNFs is saturated through the anisotropic growth to form complete hNFs. 34,35 The formation of hNFs is influenced by certain experimental parameters such as the concentration of organic and inorganic components, pHs, temperature and incubation time. [36][37][38][39][40][41][42] The formation of hNFs depends on pHs of reaction environments which is in charge of the electrostatic interaction or between enzyme molecules and enzyme and metal ion.…”

Nanomaterial-based biosensors for the detection of foodborne bacteria: a review

“…84 Therefore, the layered nano-flower (NF) technology opens a new door for enzyme immobilization, which has the advantages of enhanced loading capacity, high specific surface area, low cost and environmental protection. 87 For the detection of S. aureus , W. Yin et al 88 prepared a fused protein tdTomato-(G 4 S) 3 -CBD that contains a red fluorescent protein, tdTomato, which was incorporated together with HRP and CuSO 4 to form a three-in-one hybrid nanoflower as a chromogenic probe for the detecting S. aureus . Due to the high specific binding ability of CBD with S. aureus , and the enhanced stability and activity of HRP, the bio-hybrid nanoarchitectonics of the nanoflower allowed us to develop a novel ELISA method for efficient and sensitive detection of S. aureus .…”

Highly sensitive amperometric sensors based on laccase-mimetic nanozymes for the detection of dopamine

“…10 However, due to the high cost of laccase and its non-sufficient stability (susceptibility to changes of pH and temperature during the storage) it is advantageous to substitute laccase by nanoparticles (NPs) possessing laccase-like activity. 11–15…”