Recent Advances in DNA Nanostructures Applied in Sensing Interfaces and Cellular Imaging

Zhang, Pu; Ouyang, Yu; Zhuo, Ying; Yuan, Ruo

doi:10.1021/acs.analchem.2c04540

Cited by 12 publications

(8 citation statements)

References 161 publications

(242 reference statements)

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“…Moreover, it was found that the compound ETTC-dsDNA with a partially complementary double-stranded structure formed by base pairing had better solubility in aqueous solution than that of the compound ETTC-ssDNA, as shown in Figure E, ETTC-dsDNA had a weaker emission in the DMSO–water mixture. This is due to the double helix structure formed by a complementary structure, the hydrophilic phosphate and the sugar groups served as the skeleton at exterior, and the hydrophobic nitrogen-containing bases are paired in the internal, which improves the aqueous solubility of the compound ETTC-ssDNA . Taking advantage of this difference in the solubility (or fluorescence intensity) of ETTC-dsDNA in DMSO-water, we then attempted to tune the solubility of ETTC-dsDNA in mixtures of DMSO and water to achieve a more reasonable range of fluorescence changes.…”

Section: Resultsmentioning

confidence: 99%

“…This is due to the double helix structure formed by a complementary structure, the hydrophilic phosphate and the sugar groups served as the skeleton at exterior, and the hydrophobic nitrogen-containing bases are paired in the internal, which improves the aqueous solubility of the compound ETTC-ssDNA. 39 Taking advantage of this difference in the solubility (or fluorescence intensity) of ETTC-dsDNA in DMSO-water, we then attempted to tune the solubility of ETTC-dsDNA in mixtures of DMSO and water to achieve a more reasonable range of fluorescence changes. For this purpose, we prepared five analytes of the same concentration in this solvent mixture with 1:1, 1:2, 1:3, 1:4, and 1:5.…”

Section: Principle Of the Electrochemical Biosensing Strategymentioning

confidence: 99%

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Target-Induced AIE Effect Coupled with CRISPR/Cas12a System Dual-Signal Biosensing for the Ultrasensitive Detection of Gliotoxin

Qiao

Yuan

et al. 2023

Anal. Chem.

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Here, a novel rapid and ultrasensitive aptamer biosensor was designed for target-induced activation of AIE effect and followed by the activation of Crispr Cas12a (LbCpf1)-mediated cleavage to achieve dual-signal detection. The prepared DNA building blocks contain the target aptamer, ssDNA-Fc, and Activator1. In this system, the activation mode was divided into two steps. First, when the target interacts with the aptamers, the DNA building blocks would be disintegrated rapidly, releasing a mass of Ac1, generating ETTC-dsDNA aggregated to produce a fluorescence signal by the AIE effect. Second, with the release of Ac2, LbCpf1-crRNA was activated, which greatly improves the ssDNA-Fc cleavage efficiency to render signal amplification and ultrasensitive detection of the target. Satisfactorily, using this approach to detect gliotoxin, optimal conditions for detection was achieved for reducing the detection time to 55 min, achieving a low detection limit of 2.4 fM and a satisfactory linear in the range of 50 fM to 1 nM, which addressed the shortcoming of a weak electrochemical signal in previous sensors. The water-insoluble AIE material was coupled with DNA to obtain water-soluble ETTC-dsDNA and successfully introduced into the sensor system, with a low detection limit of 5.6 fM. Subsequently, the biosensor combined with handheld electrochemical workstation was successfully applied in the detection of gliotoxin in five actual samples, with a detection range of 32.0 to 2.09 × 108 pM. This strategy not only provides a novel and effective detection platform for mycotoxins in complex food matrices but also opens a promising avenue for various molecules detection in imaging and disease diagnosis.

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Section: Resultsmentioning

confidence: 99%

Section: Principle Of the Electrochemical Biosensing Strategymentioning

confidence: 99%

Target-Induced AIE Effect Coupled with CRISPR/Cas12a System Dual-Signal Biosensing for the Ultrasensitive Detection of Gliotoxin

Qiao

Yuan

et al. 2023

Anal. Chem.

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“…This synthetic route can effectively solve the decomposition problem of the S-containing heterocyclic end under Suzuki coupling conditions. 29 The raw material for the introduction of substituents was readily available, and the reaction time was reduced to only 1−2 h. These synthetic products of meso-SN-Cy5s were successfully confirmed by 1 H and 13 C NMR and HRMS (Figures S20− S40). Regulation of Photophysical Properties through Grafting Meso-substituents to SN-Cy5.…”

Section: Molecular Design and Synthesis Of G4-specificmentioning

confidence: 87%

“…However, stringent requirements for sample quantity, purity, and the polymorphism of the G4 structure preclude these techniques from being used to study G4 in vivo . While fluorescence sensing and imaging technologies have the advantages of cellular permeability and easy visualization. − …”

Section: Introductionmentioning

confidence: 99%

ON–OFF Fluorescent Cyanine Dye Based on a Benzothiophenyl Rotor Enables Selective Illumination of G-Quadruplexes in Mitochondria

et al. 2023

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Conventional cyanine dyes exist as "always-on" fluorescent probes leading to inevitable background signals which often limit their performance and scope of applications. To develop specific fluorescent probes with high sensitivity and robust OFF/ON switching for targeting G4s, we introduced aromatic heterocycles through conjugation with polymethine chains to construct a rotor-π system. Here, a universal strategy is presented to synthesize pentamethine cyanines with different aromatic heterocycle substituents on the meso-polymethine chain. In these probes, SN-Cy5-S is self-quenched in aqueous solution due to H-aggregation. The structure indicates that SN-Cy5-S with a flexible meso-benzothiophenyl rotor conjugated to the cyanine backbone matches adaptively with G-tetrad planes, enhancing π−π stacking and resulting in triggered fluorescence. This allows recognition of G-quadruplexes due to the synergy of disaggregation-induced emission (DIE) and inhibited twisted intramolecular charge-transfer effects. This combination leads to a robust lighting-up fluorescence response for c-myc G4 with superior fluorescence enhancement (98-fold), allowing for a low detection limit of 1.51 nM, which is much more sensitive than the previously reported DIE-based G4 probes (22−83.5 nM). In addition, the superior imaging properties and rapid internalization time (5 min) in mitochondria allow SN-Cy5-S to also have a high potential for mitochondrially targeting anti-cancer therapy.

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“…In the past few years, various methods have been employed to detect the Lin residues in food and the environment, such as high-performance liquid chromatography, fluorescence, and immunochromatographic analysis, as well as photoelectrochemical (PEC) , and electrochemical (EC) , methods. As an emerging analytical method, electrochemiluminescence (ECL) combines the advantages of electrochemistry and luminescence, such as high detection sensitivity, low background, and rapid analysis. – Nontoxic and biocompatible SnS 2 quantum dots (QDs) have served as excellent luminophores in ECL studies. For example, an ECL resonance energy transfer (ECL-RET) system was designed and applied in an immunosensor for chloramphenicol detection using SnS 2 QDs as a novel donor and flaky Ag@Au nanomaterials as acceptor .…”

Section: Introductionmentioning

confidence: 99%

AuPt Nanodonuts as a Novel Coreaction Accelerator and Luminophore for a Label-free Electrochemiluminescence Aptasensor

Li,

Luo,

Yang

et al. 2023

Anal. Chem.

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Novel and effective coreaction accelerators are of great importance in electrochemiluminescence (ECL) systems. In this work, novel AuPt nanodonuts, i.e., SnS 2 quantum dots (QDs)/Cys-AuPt heterogeneous nanorings (NRs), serve as both a highly effective coreaction accelerator and the luminophore in a label-free ECL aptasensor. The novel AuPt nanodonuts were formed by decorating SnS 2 QDs onto AuPt NR surfaces, which would promote the production of more coreactant intermediate in the SnS 2 QDs/ K 2 S 2 O 8 system. As a result, the ECL performance was greatly improved. Meanwhile, Lcysteine (L-Cys) played an important role in the combination between AuPt NRs and SnS 2 QDs, and the nanodonuts served as the matrix to load numerous lincomycin (Lin) aptamers. Under optimal conditions, the ECL aptasensor exhibited ultrasensitive detection of Lin from 1 fg/mL to 0.1 pg/mL with a limit of detection (LOD) of 0.7 fg/mL (1.72 fM).

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Recent Advances in DNA Nanostructures Applied in Sensing Interfaces and Cellular Imaging

Cited by 12 publications

References 161 publications

Target-Induced AIE Effect Coupled with CRISPR/Cas12a System Dual-Signal Biosensing for the Ultrasensitive Detection of Gliotoxin

Target-Induced AIE Effect Coupled with CRISPR/Cas12a System Dual-Signal Biosensing for the Ultrasensitive Detection of Gliotoxin

ON–OFF Fluorescent Cyanine Dye Based on a Benzothiophenyl Rotor Enables Selective Illumination of G-Quadruplexes in Mitochondria

AuPt Nanodonuts as a Novel Coreaction Accelerator and Luminophore for a Label-free Electrochemiluminescence Aptasensor

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