AuPt Nanodonuts as a Novel Coreaction Accelerator and Luminophore for a Label-free Electrochemiluminescence Aptasensor

Abstract

Novel and effective coreaction accelerators are of great importance in electrochemiluminescence (ECL) systems. In this work, novel AuPt nanodonuts, i.e., SnS 2 quantum dots (QDs)/Cys-AuPt heterogeneous nanorings (NRs), serve as both a highly effective coreaction accelerator and the luminophore in a label-free ECL aptasensor. The novel AuPt nanodonuts were formed by decorating SnS 2 QDs onto AuPt NR surfaces, which would promote the production of more coreactant intermediate in the SnS 2 QDs/ K 2 S 2 O 8 system. As a result, the ECL performance was greatly improved. Meanwhile, Lcysteine (L-Cys) played an important role in the combination between AuPt NRs and SnS 2 QDs, and the nanodonuts served as the matrix to load numerous lincomycin (Lin) aptamers. Under optimal conditions, the ECL aptasensor exhibited ultrasensitive detection of Lin from 1 fg/mL to 0.1 pg/mL with a limit of detection (LOD) of 0.7 fg/mL (1.72 fM).

Regulating Reactive Oxygen Species over M–N–C Single-Atom Catalysts for Potential-Resolved Electrochemiluminescence

“…Importantly, these intermediates exhibit distinct lifetimes and oxidation abilities, such as ·OH (with lifetimes ranging from approximately 10 –9 to 10 –6 s; E 0 (OH·/OH – ) = +1.9 to +2.7 V NHE ) and O 2 ·– (with a lifetime of approximately 60 s; E 0 (O 2 ·– /O 2 2– ) = ∼+1.69 V NHE ), which undergo different ECL reaction pathways, contributing to potential-resolved luminol ECL behaviors. However, rationally designing advanced coreaction accelerators to achieve the controlled and efficient generation of ROS by activating dissolved O 2 remains a challenge for developing potential-resolved ECL using a single luminophore. , …”

Electrochemical Detection of a Breast Cancer Biomarker with an Amine-Functionalized Nanocomposite Pt–Fe3O4–MWCNTs–NH2 as a Signal-Amplifying Label

“…The nanomaterials can act as a pseudoenzyme by mimicking the enzymatic action. Therefore, nanomaterial-based signal amplification can improve the stability and sensitivity of the immunosensors. , The utilization of the nanocomposite of metal and metal oxide in electrochemical immunosensors has gained significant attention for biomolecule detection. − Among metals, platinum nanoparticles (Pt NPs) have been regarded as the most promising due to their distinctive physical and chemical characteristics such as excellent catalytic capabilities, ease of preparation, biocompatibility, and good stability. , Fe 3 O 4 nanoparticles have intrinsic peroxidase-like activity with good biocompatibility and a large surface area. , Fe 3 O 4 exhibits peroxidase-like activity, primarily due to its ability to facilitate the decomposition of hydrogen peroxide (H 2 O 2 ), with the active catalytic center being Fe 2+/ Fe 3+ . Initially, Fe 3+ undergoes partial reduction to Fe 2+ , which serves to activate H 2 O 2 and generate hydroxyl radicals ( • OH).…”

The potential of an MOF accelerator in an electrochemiluminescence system for sensitive detection of menthol enantiomers

“…21,22 This accelerator interacts with the co-reactant, generating more active intermediate states, thereby bolstering the ECL signal. 23,24 Recently, a number of coreaction accelerators have been put forward for different ECL coreaction systems, including nanomaterials, 25,26 single-atom materials, 27,28 organic small molecules, 29,30 and so on. However, the development of coreaction accelerators that are structurally simple, cost-effective, stable, and low in toxicity continues to pose a challenge.…”