Abstract:Here, a novel rapid and ultrasensitive aptamer biosensor
was designed
for target-induced activation of AIE effect and followed by the activation
of Crispr Cas12a (LbCpf1)-mediated cleavage to achieve dual-signal
detection. The prepared DNA building blocks contain the target aptamer,
ssDNA-Fc, and Activator1. In this system, the activation mode was
divided into two steps. First, when the target interacts with the
aptamers, the DNA building blocks would be disintegrated rapidly,
releasing a mass of Ac1, generati… Show more
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