Recent Advances in Andrology Research: Physiopathology and Clinical Application to Fertility and Infertility

Hafez, B.; Goff, Lynda J.; Hafez, Saad Dean

doi:10.3109/01485019708987916

Cited by 8 publications

(8 citation statements)

References 63 publications

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“…Disruptions to the biochemical and biophysical reactions following ejaculation are a major cause of infertility in men [43,15]. Since seminal fluid has important roles in spermatozoon survival and overall fertilization success, its impairment can be directly connected to infertility.…”

Section: Introductionmentioning

confidence: 99%

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Jayaraman

Ghosh

Sengupta

et al. 2014

J Assist Reprod Genet

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Purpose The aim of this study was to analyze the seminal plasma of patients with idiopathic/male factor infertility and healthy controls with proven fertility by NMR spectroscopy, with a hope of establishing difference in biomarker profiles, if any, between the groups. Methods A total of 103 subjects visiting the infertility clinic of Manipal University with normozoospermic parameters, oligozoospermia, asthenozoospermia, azoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria. Seminal plasma was subjected to NMR spectroscopy at a 700 MHz

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Section: Introductionmentioning

confidence: 99%

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Jayaraman

Ghosh

Sengupta

et al. 2014

J Assist Reprod Genet

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“…Since the late 1970s, it has been known that methylxanthine derivates act as motility stimulants in mammalian spermatozoa of various species [5,12,22,28]. These compounds are gaining wider clinical use [15], but information about their effects on the main sperm functional parameters has remained sparse [29]. This study focused on the effects of pentoxifylline and other agents that interact with cAMP signal transduction pathway on responses to hypoosmotic shock and percentage of motile cells, parameters in which the functional integrity of sperm membrane is strongly involved.…”

Section: Discussionmentioning

confidence: 99%

“…Pentoxifylline is a methylxanthine used in the treatment of infertile males [15] and in human and animal assisted fertilization techniques. Pharmacological assays employing reagents whose mechanism of action is known are useful tools for elucidating details relative to the modulation of sperm functional activity.…”

mentioning

confidence: 99%

Influence of Pentoxifylline on Sperm Membrane Functional Integrity

Ponce¹,

M²,

Rd³

et al. 1999

Archives of Andrology

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In epididymal mouse spermatozoa, the effects of dibutyryl cyclic adenosine monophosphate 1 mmol/L (dbcAMP), pentoxifylline 5 mmol/L (PX), and/or mastoparan 50 mumol/L (MT) were evaluated for the following parameters: percentage of motile cells and response to hypoosmotic shock (HOS). The gametes were incubated during 80 min (A) or 200 min (B) in Tyrode's medium, and the drugs were added during the last 20 min. In A, dbcAMP + PX (61.5 +/- 5.4%; n = 10) enhanced and MT decreased significantly the population of motile cells (13.4 +/- 5.4%; n = 6) (control: 47.6 +/- 3.9%; n = 11). In B, PX significantly increased this parameter and MT plus PX also exerted a significant detrimental effect. Responses to HOS dropped significantly in the presence of PX + MT in A or in B; in this latter condition a similar decrease was evoked by MT alone. A positive correlation between percentages of swollen and motile spermatozoa was detected in A or in B in samples incubated with PX (r = .58, n = 11 and r = .76, n = 10; p < .05, respectively). These results that support that, in mouse sperm tail, PX would preserve functional membrane integrity, a relevant condition for adequate motility.

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“…The evaluation of male-factor infertility has become more important and informative since new diagnostic techniques and therapeutic options have become available. Poor sperm motility has been considered as one of the major causes of male infertility [ 1 ]. It is highly probable that the respiratory dysfunction of mitochondria causes a decline in motility [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Association in the Maintenance of the Mitochondrial Microenvironment and Sperm Capacity

Thomas

Chen

Hung

et al. 2021

Oxidative Medicine and Cellular Longevity

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Sperm motility is one of the major determinants of male fertility. Since sperm need a great deal of energy to support their fast movement by active metabolism, they are thus extremely vulnerable to oxidative damage by the reactive oxygen species (ROS) and other free radicals generated as byproducts in the electron transport chain. The present study is aimed at understanding the impact of a mitochondrial oxidizing/reducing microenvironment in the etiopathology of male infertility. We detected the mitochondrial DNA (mtDNA) 4,977 bp deletion in human sperm. We examined the gene mutation of ATP synthase 6 (ATPase6 m.T8993G) in ATP generation, the gene polymorphisms of uncoupling protein 2 (UCP2, G-866A) in the uncoupling of oxidative phosphorylation, the role of genes such as manganese superoxide dismutase (MnSOD, C47T) and catalase (CAT, C-262T) in the scavenging system in neutralizing reactive oxygen species, and the role of human 8-oxoguanine DNA glycosylase (hOGG1, C1245G) in 8-hydroxy-2 ′ -deoxyguanosine (8-OHdG) repair. We found that the sperm with higher motility were found to have a higher mitochondrial membrane potential and mitochondrial bioenergetics. The genotype frequencies of UCP2 G-866A, MnSOD C47T, and CAT C-262T were found to be significantly different among the fertile subjects, the infertile subjects with more than 50% motility, and the infertile subjects with less than 50% motility. A higher prevalence of the mtDNA 4,977 bp deletion was found in the subjects with impaired sperm motility and fertility. Furthermore, we found that there were significant differences between the occurrences of the mtDNA 4,977 bp deletion and MnSOD (C47T) and hOGG1 (C1245G). In conclusion, the maintenance of the mitochondrial redox microenvironment and genome integrity is an important issue in sperm motility and fertility.

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Recent Advances in Andrology Research: Physiopathology and Clinical Application to Fertility and Infertility

Cited by 8 publications

References 63 publications

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Influence of Pentoxifylline on Sperm Membrane Functional Integrity

Genetic Association in the Maintenance of the Mitochondrial Microenvironment and Sperm Capacity

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