Recent Advancements in Decellularized Matrix-Based Biomaterials for Musculoskeletal Tissue Regeneration

Kim, Hyunbum; Kim, Yunhye; Fendereski, Mona; Hwang, Nathaniel S.; Hwang, Yongsung

doi:10.1007/978-981-13-0947-2_9

Cited by 11 publications

(11 citation statements)

References 79 publications

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“…Decellularization technique involves chemical, enzymatic, physical and combined methods, which can remove cells and preserve extracellular matrix integrity 38 . Triton X‐100, a non‐ionic surfactant, is a relatively mild agent for decellularization, which is usually used in combination with NH 4 OH 38 . A recent study evaluated various methods for the decellularization of periodontal ligament cell sheets 39 .…”

Section: Discussionmentioning

confidence: 99%

Decellularized matrix could affect the proliferation and differentiation of periodontal ligament stem cells in vitro

Huang

Liu

et al. 2021

J of Periodontal Research

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Objective and Background Recently, decellularized matrix (DCM) is considered as a new biomaterial for tissue regeneration. To explore the possible application of DCM in periodontal regeneration, the effect of DCM from three different cells on the proliferation and differentiation of human periodontal ligament stem cells (PDLSCs) was investigated. Methods DCM derived from human periodontal ligament cells (PDLCs), dental pulp cells (DPCs), and gingival fibroblasts (GFs) were fabricated using Triton X‐100/NH4OH combined with DNase I. Allogeneic PDLSCs were cultured on PDLC‐DCM, DPC‐DCM, and GF‐DCM, respectively. The proliferative capacity of PDLSCs was evaluated by PicoGreen assay kit. The expression of alkaline phosphatase (ALP), runt‐related transcription factor‐2 (RUNX2), osteocalcin (OCN), collagen I (COL1), periostin (POSTN), and cementum protein 1 (CEMP1) were detected by qRT‐PCR and western blotting. Results PDLC‐DCM, DPC‐DCM, and GF‐DCM had similar and integrated networks of extracellular matrix, as well as significantly decreased DNA content. Compared with control group in which PDLSCs were directly seeded in culture plates, PDLC‐DCM, DPC‐DCM, and GF‐DCM promoted the proliferation of re‐seeded PDLSCs. Additionally, PDLSCs on DCM exhibited higher mRNA and protein expression levels of ALP, RUNX2, OCN, and COL1. The expression of POSTN in PDLC‐DCM group was significantly higher than control group at both mRNA and protein levels. Conclusions PDLC‐DCM, DPC‐DCM, and GF‐DCM could enhance the proliferation of PDLSCs. PDLC‐DCM facilitated osteogenic differentiation and periodontal ligament differentiation of PDLSCs, while DPC‐DCM and GF‐DCM promoted osteogenic differentiation.

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Section: Discussionmentioning

confidence: 99%

Decellularized matrix could affect the proliferation and differentiation of periodontal ligament stem cells in vitro

Huang

Liu

et al. 2021

J of Periodontal Research

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“…This improves the efficiency of acellular decellularization ( Rana et al, 2017 ). A combination of an enzymic reagent and a chelating agent, such as trypsin and EDTA, is frequently used to selectively destroy cell adhesion proteins on the carbon side of arginine and lysine ( Kim et al, 2018 ).…”

Section: Preparation Of the Ecm Scaffoldmentioning

confidence: 99%

Preparation and Application of Decellularized ECM-Based Biological Scaffolds for Articular Cartilage Repair: A Review

Zhang

Long

et al. 2022

Front. Bioeng. Biotechnol.

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Cartilage regeneration is dependent on cellular-extracellular matrix (ECM) interactions. Natural ECM plays a role in mechanical and chemical cell signaling and promotes stem cell recruitment, differentiation and tissue regeneration in the absence of biological additives, including growth factors and peptides. To date, traditional tissue engineering methods by using natural and synthetic materials have not been able to replicate the physiological structure (biochemical composition and biomechanical properties) of natural cartilage. Techniques facilitating the repair and/or regeneration of articular cartilage pose a significant challenge for orthopedic surgeons. Whereas, little progress has been made in this field. In recent years, with advances in medicine, biochemistry and materials science, to meet the regenerative requirements of the heterogeneous and layered structure of native articular cartilage (AC) tissue, a series of tissue engineering scaffolds based on ECM materials have been developed. These scaffolds mimic the versatility of the native ECM in function, composition and dynamic properties and some of which are designed to improve cartilage regeneration. This review systematically investigates the following: the characteristics of cartilage ECM, repair mechanisms, decellularization method, source of ECM, and various ECM-based cartilage repair methods. In addition, the future development of ECM-based biomaterials is hypothesized.

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“…Overall, the use of dECM has become a whole new paradigm in bioprinting for tissue engineering. Details of using dECM for bioprinting have also been summarized comprehensively in a few well-written review articles (Baiguera et al, 2020;Dzobo et al, 2019;Kim et al, 2018aKim et al, , 2019. Therefore, we only summarized a few musculoskeletal-related applications for dECM in this review without further expanding the topic (Table 2).…”

Section: Decellularized Extracellular Matrix (Decm)mentioning

confidence: 99%

Hybprinting for musculoskeletal tissue engineering

Kim

Chen

et al. 2022

iScience

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Recent Advancements in Decellularized Matrix-Based Biomaterials for Musculoskeletal Tissue Regeneration

Cited by 11 publications

References 79 publications

Decellularized matrix could affect the proliferation and differentiation of periodontal ligament stem cells in vitro

Decellularized matrix could affect the proliferation and differentiation of periodontal ligament stem cells in vitro

Preparation and Application of Decellularized ECM-Based Biological Scaffolds for Articular Cartilage Repair: A Review

Hybprinting for musculoskeletal tissue engineering

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