Real-Time RT-PCR assay to quantify the expression of fum1 and fum19 genes from the Fumonisin-producing Fusarium verticillioides

López‐Errasquín, Elena; Vázquez, Covadonga; Jiménez, Misericordia; González-Jaén, M. Teresa

doi:10.1016/j.mimet.2006.09.007

Cited by 66 publications

(55 citation statements)

References 22 publications

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“…Many studies have demonstrated that the expression of Fum1 is correlated with fumonisin production (Proctor et al 1999;Seo et al 2001;L opez-Errasqu ın et al 2007;Jurado et al 2008). In our study we found variation in the expression of Fum1 transcripts, with a maximum increase under red, yellow and royal blue (200 lx) light, and a decrease under white, green and SW.…”

Section: Discussionsupporting

confidence: 54%

Influence of light on growth, conidiation and fumonisin production by Fusarium verticillioides

et al. 2012

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Section: Discussionsupporting

confidence: 54%

Influence of light on growth, conidiation and fumonisin production by Fusarium verticillioides

et al. 2012

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“…FUM1 and FUM19 transcripts were observed from F. verticillioides cultures incubated at 20°C for 14 days and found to be linearly correlated to fumonisin production (López-Errasquín et al, 2007). These findings have been confirmed by de Oliveira Rocha et al (2011) in F. verticillioides 7 day-old cultures grown at 25°C.…”

Section: Introductionsupporting

confidence: 54%

“…verticillioides strains used in this study were recovered from maize and were confirmed to be fumonisin-producers (Etcheverry et al, 2009;Moretti et al, 1995). They showed different patterns of fumonisin production: ITEM 1744 had a classical pattern of FBs produced (FB 1 > FB 2 > FB 3 ; de Oliveira Rocha et al, 2011;López-Errasquín et al, 2007) while ITEM 10027 presented one of the minor chemotypes: FB 1 >FB 3 >FB 2 (Szecsi et al, 2010), in accord with our previous data (ITEM1744: 2800 FB 1 >521 FB 2 >480 FB 3 μg/kg; ITEM 10027: 7720 FB 1 >2180 FB 3 > 2070 FB 2 μg/kg) (Lazzaro et al, 2012). Water activity significantly affected fumonisin production with a w =0.990 supporting more production than a w =0.955.…”

Section: Discussionmentioning

confidence: 92%

FUM and BIK gene expression contribute to describe fumonisin and bikaverin synthesis in Fusarium verticillioides

Lazzaro

Busman

Battilani

et al. 2012

International Journal of Food Microbiology

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Lazzaro, I.; Busman, M.; Battilani, P.; and Butchko, R. A. E., "FUM and BIK gene expression contribute to describe fumonisin and bikaverin synthesis in Fusarium verticillioides" (2012 Fusarium verticillioides is a maize pathogen that produces toxic secondary metabolites, including fumonisins and bikaverin. The regulation of biosynthetic gene expression and the production of these metabolites are not fully understood and in this study we investigated the influence of water activity (0.955 and 0.990) on the expression of 5 genes (FUM3-FUM8-FUM13-FUM14 and BIK1) in F. verticillioides strains after 14 and 21 days incubation. Fumonisin production and biosynthetic gene expression were greatest at a w = 0.990, and the same trend was observed for bikaverin production, and BIK1 expression. FUM3 and FUM14 were the most highly expressed genes and were positively correlated with the production of FB 1 , FB 2 and FB 3 . When FUM14 is more highly expressed than FUM3 the amount of FB 3 quantified is higher with respect to FB 1 ; this could be explained by the role of FUM3 in the hydroxylation of FB 3 to FB 1 .

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“…Recent reports indicate significant correlation between FUM1 transcripts, quantified by real-time reverse transcription-PCR (RT-PCR), and phenotypic fumonisin production (6,7). The quantitation of FUM1 transcripts by real-time RT-PCR permits a sensitive and specific approach to evaluate the effects of various treatments on fumonisin biosynthesis.…”

mentioning

confidence: 99%

Relationship between Solute and Matric Potential Stress, Temperature, Growth, and FUM1 Gene Expression in Two Fusarium verticillioides Strains from Spain

Jurado

Marín

Magan

et al. 2008

Appl Environ Microbiol

105

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The objective of this work was to study the effect of ecophysiological factors on fumonisin gene expression and growth in Fusarium verticillioides. The effects of ionic and nonionic solute water potentials, matric potential, and temperature on in vitro mycelial growth rates and on expression of the FUM1 gene, involved in fumonisin biosynthesis, were examined. FUM1 transcript levels were quantified using a specific real-time reverse transcription-PCR (RT-PCR) protocol. Low temperature and water stress reduced fungal growth. Water stress increased FUM1 transcript levels, especially in the case of stress caused by nonionic solute. The temporal kinetic assays showed that water stress had opposite effects on fungal growth versus FUM1 expression. These results indicate that water stress may be an important factor for fumonisin accumulation, particularly in the later phases of maize colonization when water availability decreases. The quantitative RT-PCR methods described here provide a valuable tool for investigating the ecophysiological basis for fumonisin gene expression and ultimately may lead to more effective control strategies for this important mycotoxigenic pathogen.During the life cycle of Fusarium verticillioides in maize, the pathogen colonizes soil and survives effectively on crop residue and spores are dispersed predominantly via rain splash and sometimes by wind. The pathogen's life cycle is significantly influenced by environmental factors, especially water availability and temperature (14). In terrestrial ecosystems, water availability can be expressed by the total water potential (⌿ t ), a measure of the fraction of the total water content available for microbial growth in pascals (10). This is the sum of three factors: (i) osmotic or solute potential (⌿ s ) due to the presence of ions or other solutes, (ii) matric potential (⌿ m ) due directly to forces required to remove water bound to the matrix (e.g., soil), and (iii) turgor potential of microbial cells balancing their internal status with the external environment. The influences of both ⌿ s and ⌿ m were of interest in this study because growth on crop residue and in ripening silks is determined predominantly by tolerance to solute potential, while growth in soil is determined mainly by matric potential, except in saline soils. The effects of ionic and nonionic ⌿ s stress on germination, growth, and fumonisin production by strains of F. verticillioides and Fusarium proliferatum have been determined in vitro and in stored maize grain (13). However, no information is available on the effect of soil ⌿ m water stress and how F. verticillioides responds to such stress in terms of growth capability and production of fumonisins.Fungi vary in their ability to tolerate ⌿ m stress. For example, basidiomycetes such as Rhizoctonia solani and the cultivated mushrooms (Agaricus bisporus and Pleurotus ostreatus) are very sensitive to matric forces compared to ionic or nonionic ⌿ s stress (1, 11). Interestingly, xerotolerant mycotoxigenic species such as Aspergill...

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Real-Time RT-PCR assay to quantify the expression of fum1 and fum19 genes from the Fumonisin-producing Fusarium verticillioides

Cited by 66 publications

References 22 publications

Influence of light on growth, conidiation and fumonisin production by Fusarium verticillioides

Influence of light on growth, conidiation and fumonisin production by Fusarium verticillioides

FUM and BIK gene expression contribute to describe fumonisin and bikaverin synthesis in Fusarium verticillioides

Relationship between Solute and Matric Potential Stress, Temperature, Growth, and FUM1 Gene Expression in Two Fusarium verticillioides Strains from Spain

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