FUM and BIK gene expression contribute to describe fumonisin and bikaverin synthesis in Fusarium verticillioides

Lazzaro, Irene; Busman, Mark; Battilani, Paola; Butchko, Robert A. E.

doi:10.1016/j.ijfoodmicro.2012.10.004

Cited by 20 publications

(13 citation statements)

References 40 publications

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“…FUM1 expression can be induced and repressed by NaCl at low and high concentrations ( Jurado et al, 2008 ). For F. proliferatum , FUM1 transcription was induced under non-ionic water stress at 15–35°C in F. proliferatum culture ( Lazzaro et al, 2012a ). FUM8 as an aminotransferase gene is transcriptionally regulated also ( Seo et al, 2001 ).…”

Section: Discussionmentioning

confidence: 99%

Influence of Butylated Hydroxyanisole on the Growth, Hyphal Morphology, and the Biosynthesis of Fumonisins in Fusarium proliferatum

Jian

Chen

et al. 2016

Front. Microbiol.

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Fusarium proliferatum as a common fungus pathogen in foods can produce toxic fumonisins, which can cause animal diseases and increase risks of human cancers. On contrary, butylated hydroxyanisole (BHA) as a synthetic antioxidant offers a clue for preventing growth of fungal species and inhibiting production of mycotoxins. Unfortunately, information of the inhibitory mechanism of BHA on Fusarium species is still limited. In this study, influence of BHA treatment on growth and inhibition of fumonisin production in relation to the expression of the fumonisin biosynthesis-related genes of the F. proliferatum ZYF was investigated, which revealed that BHA had a negative influence on growth and fumonisin production of F. proliferatum. To further elucidate the mechanism of BHA on the growth of F. proliferatum, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine the F. proliferatum hyphae. The BHA treatment induced the loss of cytoplasm and cellular constituents, as well as distortion of mycelia, but it did not directly degrade the fumonisin. Furthermore, the BHA treatment markedly inhibited the expressions of FUM1 (a polyketide synthase encoding gene) and FUM8 (an aminotransferase encoding gene) genes, which resulted in the depression of metabolic pathway of F. proliferatum. The transcriptional analyses of the FUM1 and FUM8 genes confirmed a correlation between the fumonisin production and its gene expression. This study provided some insights into mechanisms of production of fumonisin and feasible prevention to reduce fumonisin contamination in favor of human and animal health.

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Section: Discussionmentioning

confidence: 99%

Influence of Butylated Hydroxyanisole on the Growth, Hyphal Morphology, and the Biosynthesis of Fumonisins in Fusarium proliferatum

Jian

Chen

et al. 2016

Front. Microbiol.

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“…Fumonisins may also cause oesophageal (Kamangar et al 2009;Kigen et al 2017) and renal (Müller et al 2012;Wu et al 2014;Barnett and Cummings 2018) cancers as well as neural tube defects (Wu et al 2014;Ponce-García et al 2018;Lumsangkul et al 2019). A deeper understanding of the molecular regulation of fumonisin biosynthesis in toxigenic fusaria (Butchko et al 2012;Lazzaro et al 2012;Woloshuk and Shim 2013;Gu et al 2017;Gil-Serna et al 2019) may lead to the development of atoxigenic strains with possible biocontrol potential or to the utilization of RNA interference technologies to silence mycotoxin biosynthesis genes (Alberts et al 2016).…”

Section: Introductionmentioning

confidence: 99%

FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides

Szabó

Pákozdi

Murvai

et al. 2020

Appl Microbiol Biotechnol

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FvatfA from the maize pathogen Fusarium verticillioides putatively encodes the Aspergillus nidulans AtfA and Schizasaccharomyces pombe Atf1 orthologous bZIP-type transcription factor, FvAtfA. In this study, a ΔFvatfA deletion mutant was constructed and then genetically complemented with the fully functional FvatfA gene. Comparing phenotypic features of the wild-type parental, the deletion mutant and the restored strains shed light on the versatile regulatory functions played by FvAtfA in (i) the maintenance of vegetative growth on Czapek-Dox and Potato Dextrose agars and invasive growth on unwounded tomato fruits, (ii) the preservation of conidiospore yield and size, (iii) the orchestration of oxidative (H 2 O 2 , menadione sodium bisulphite) and cell wall integrity (Congo Red) stress defences and (iv) the regulation of mycotoxin (fumonisins) and pigment (bikaverin, carotenoid) productions. Expression of selected biosynthetic genes both in the fumonisin (fum1, fum8) and the carotenoid (carRA, carB) pathways were down-regulated in the ΔFvatfA strain resulting in defected fumonisin production and considerably decreased carotenoid yields. The expression of bik1, encoding the polyketide synthase needed in bikaverin biosynthesis, was not up-regulated by the deletion of FvatfA meanwhile the ΔFvatfA strain produced approximately ten times more bikaverin than the wild-type or the genetically complemented strains. The abolishment of fumonisin production of the ΔFvatfA strain may lead to the development of new-type, biology-based mycotoxin control strategies. The novel information gained on the regulation of pigment production by this fungus can be interesting for experts working on new, Fusarium-based biomass and pigment production technologies. Key points • FvatfA regulates vegetative and invasive growths of F. verticillioides. • FvatfA also orchestrates oxidative and cell wall integrity stress defenses. • The ΔFvatfA mutant was deficient in fumonisin production. • FvatfA deletion resulted in decreased carotenoid and increased bikaverin yields.

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“…In Fv , genes responsible for the biosynthesis of bikaverin, a polyketide structurally similar to fatty acids (Hopwood and Sherman, ), were up‐regulated in the Δfac1 mutant (http://onlinelibrary.wiley.com/doi/10.1111/mpp.12272/suppinfo). Bikaverin is one of the main toxic secondary metabolites with antibiotic activity produced by Fv (Lazzaro et al ., ). Interestingly, the Fv ‐unique component of the cAMP–PKA pathway also regulated several genes involved in fatty acid metabolism (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…In Fv, genes responsible for the biosynthesis of bikaverin, a polyketide structurally similar to fatty acids (Hopwood and Sherman, 1990), were up-regulated in the Δfac1 mutant (Table S2). Bikaverin is one of the main toxic secondary metabolites with antibiotic activity produced by Fv (Lazzaro et al, 2012).…”

Section: The Diverged Camp-pka Pathway Controls Species-specific Biolmentioning

confidence: 99%

“…Although Fg causes head blight on Triticum aestivum (wheat) and Hordeum vulgare (barley), and ear and stalk rot on Zea mays (maize; Goswami and Kistler, 2004;Leslie and Summerell, 2006;Sutton, 1982), Fv primarily causes stalk and ear rot in maize and Sorghum bicolor (sorghum) (Leslie and Summerell, 2006). In addition to causing yield losses, both fungi produce species-specific mycotoxins, such as deoxynivalenol (DON) and aurofusarin in Fg (Desjardins et al, 1993;Goswami and Kistler, 2004) and fumonisin B1 and bikaverin in Fv (Kedera et al, 1999;Lazzaro et al, 2012;Sydenham et al, 1990), which pose serious threats to animal and human health.…”

Section: Introductionmentioning

confidence: 99%

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Conservation and divergence of the cyclic adenosine monophosphate–protein kinase A (cAMP–PKA) pathway in two plant‐pathogenic fungi: Fusarium graminearum and F. verticillioides

Guo

Breakspear

Zhao

et al. 2015

Molecular Plant Pathology

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SummaryThe cyclic adenosine monophosphate–protein kinase A (cAMP–PKA) pathway is a central signalling cascade that transmits extracellular stimuli and governs cell responses through the second messenger cAMP. The importance of cAMP signalling in fungal biology has been well documented and the key conserved components, adenylate cyclase (AC) and the catalytic subunit of PKA (CPKA), have been functionally characterized. However, other genes involved in this signalling pathway and their regulation are not well understood in filamentous fungi. Here, we performed a comparative transcriptomics analysis of AC and CPKA mutants in two closely related fungi: Fusarium graminearum (Fg) and F. verticillioides (Fv). Combining available Fg transcriptomics and phenomics data, we reconstructed the Fg cAMP signalling pathway. We developed a computational program that combines sequence conservation and patterns of orthologous gene expression to facilitate global transcriptomics comparisons between different organisms. We observed highly correlated expression patterns for most orthologues (80%) between Fg and Fv. We also identified a subset of 482 (6%) diverged orthologues, whose expression under all conditions was at least 50% higher in one genome than in the other. This enabled us to dissect the conserved and unique portions of the cAMP–PKA pathway. Although the conserved portions controlled essential functions, such as metabolism, the cell cycle, chromatin remodelling and the oxidative stress response, the diverged portions had species‐specific roles, such as the production and detoxification of secondary metabolites unique to each species. The evolution of the cAMP–PKA signalling pathway seems to have contributed directly to fungal divergence and niche adaptation.

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FUM and BIK gene expression contribute to describe fumonisin and bikaverin synthesis in Fusarium verticillioides

Cited by 20 publications

References 40 publications

Influence of Butylated Hydroxyanisole on the Growth, Hyphal Morphology, and the Biosynthesis of Fumonisins in Fusarium proliferatum

Influence of Butylated Hydroxyanisole on the Growth, Hyphal Morphology, and the Biosynthesis of Fumonisins in Fusarium proliferatum

FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides

Conservation and divergence of the cyclic adenosine monophosphate–protein kinase A (cAMP–PKA) pathway in two plant‐pathogenic fungi: Fusarium graminearum and F. verticillioides

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