Real time PCR based on Fluorescent Quenching of Mercaptoacetic Acid-Modified CdTe Quantum Dots for Ultrasensitive Specific Detection of Nucleic Acids

Cui, Daxiang; Li, Qing; Huang, Peng; Wang, Kan; Kong, Ying; Zhang, Hong; You, Xiaogang; He, Rong; Shi, Hua; Wang, Jingping; Asahi, Toru; Gao, Feng; Osaka, T.

doi:10.5101/nbe.v2i1.p44-54

Cited by 13 publications

(13 citation statements)

References 36 publications

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“…[8,9] Recently, QDs have been used as additives to improve PCR yield and specificity. [10][11][12][13][14][15] Furthermore, QDs demonstrated very obvious hot-start features similar to those of commercial hot-start polymerase. [16,17] This report described a simple/economical, fast PCR based on the modifications of the cycling conditions with the help of cadmium telluride (CdTe) QDs.…”

Section: Introductionmentioning

confidence: 99%

CdTe quantum dots accelerate the speed of Pfu-based polymerase chain reaction

Sang

Yang

Hexiang

et al. 2013

Journal of Experimental Nanoscience

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It has not been previously reported that the speed of polymerase chain reaction (PCR) can be accelerated by quantum dots (QDs). In the present work, the addition of cadmium telluride (CdTe) QDs resulted in significant time-saving compared with the conventional PCR. The reaction time could be significantly shortened from 143 to 46 min without compromising the general efficiency in a PCR. CdTe QD-facilitated fast PCR also displayed excellent hot-start effects.

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Section: Introductionmentioning

confidence: 99%

CdTe quantum dots accelerate the speed of Pfu-based polymerase chain reaction

Sang

Yang

Hexiang

et al. 2013

Journal of Experimental Nanoscience

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“…But they are not capable to be used for supersensitivity detection and real time monitoring due to their comparative low fluorescence intensity and low photostability. Recently, fluorescent nanoparticles including quantum dots and fluorescent silica particles, as fluorescent probes, have been developed and shown a lot of advantages [1][2][3][4][5][6][7][8][9][10][11]. As a lot of fluorescent dye molecules encapsulated in the silica matrix that also severs to protect dye molecules from photodamaging oxidation, the silica fluorescent nanoparticles are extremetly bright, photostable and chemical stable [12,13].…”

Section: Introductionmentioning

confidence: 99%

Time-Resolved Fluorescence Immunoassay of Mouse IgG using Europium(Ш) Chelate-doped Silica Nanoparticles

Yin¹,

Zhang²,

Liu³

et al. 2011

Nano BioMed ENG

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Nanoparticle labes conjuaged with biomolecules have been used in a variety of different assay application. We investigated the possibility of using europium(III) chated-doped silica nanoparticle conjugated with streptavidin (SA) to detect mouse IgG by time-resolved fluorescence immunoassay (TRFIA). Results demonstrate that the nanoparticle is a novel kind of superior fluourscent probe which could be effectively applied in time-resolved fluorescent immunoassay with high detective sensitivity. In the present study, the lowest detection limit of mouse IgG is 34 pg mL -1 .

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“…One promising application is for DNA amplification by using the polymerase chain reaction (PCR). Recently, Daxiang Cui et al reported a new PCR system based on mercaptoacetic acid-modified CdTe nanocrystals (mQDs) and can improve the sensitivity and specificity of PCR within less than 1.33 mg/mL [12], but compared with the conventional PCR assays, the concept of miniaturizing and automating PCR systems through digital microfluidics and advanced microfabrication techniques has attracted a great deal of attention because of the potential to dramatically improve the speed, portability, cost, sensitivity and specificity. For example, Zhishan Hua et al successfully developed a multiplexed real-time PCR system using electrowetting-based digital microfluidics [13].…”

Section: Nucleic Acid Analysismentioning

confidence: 99%

Biological Application of Digital Microfluidics Technology

Liu¹,

Deng²,

Qin³

et al. 2010

Nano BioMed ENG

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Digital microfluidics technology offers a platform for developing diagnostic applications with the advantages of portability, sample and reagent volume reduction, faster analysis, increased automation, low power consumption, compatibility with mass manufacturing and high throughput. In addition to diagnostics, digital microfluidics is finding use in nucleic acid analysis, peptide and protein analysis, cell analysis, drug analysis and delivery and immunization analysis. In this review, we describe these applications, their implementation, and associated design issues. As other review in the digital microfluidics technology, there have been and will be unexpected developments as DMF matures, but we predict that the future is bright for this promising technology at the last section.Keywords: Digital microfluidics technology; Nucleic acid analysis; Peptide and protein analysis; Cell analysis; Drug analysis and delivery Citation: B. Liu et al. Biological Application of Digital Microfluidics Technology. Nano Biomed Eng. 2010, 2(2), 149-154. DOI: 10.5101/nbe.v2i2.p149-154. The advent and development of digital microfluidics technologyTraditional (continuous-flow) microfluidic technologies are based on the continuous flow of liquid through microfabricated channels [1]. Continuous-flow systems are inherently difficult to integrate because the parameters that govern flow field (e.g. pressure, fluid resistance, electric field strength) vary along the flow-path, making the flow at any location dependent upon the properties of the entire system.The concept of digital microfluidics (DMF) arose in the late 1990s and involves the manipulation of discrete volumes of liquids on a surface. Manipulation of droplets can occur through various mechanisms, including electrowetting [2], dielectrophoresis [3], thermocapillary transport [4] and surface acoustic wave transport [5]. DMF was popularized in the early 2000s by Fair and coworkers [6] and Kim and coworkers [7] at Duke and UCLA, respectively. The technique was explained as being a phenomenon driven by surface tension, and was called "electrowetting" or "electrowetting ondielectric" (EWOD). A detailed review of electrowetting basics can be found in the work of Mugele [8]. In addition, work on simulation and modeling of dropletbased electrowetting has been reported by Biddut Bhattacharjee and Homayoun Najjaran [9].In contrast to continuous-flow biochips, digital microfluidic biochips platform is under software-driven electronic control, eliminating the need for mechanical tubes, pumps, and valves. Moreover, because each droplet can be controlled independently, these "digital" systems also have dynamic reconfigurability, whereby groups of cells in a microfluidic array can be reconfigured to change their functionality during the concur- Application of digital microfluidics technology in bioanalysisTo accomplish a digital microfluidics device it requires a hierarchical taxonomy. At the top level, applications are scaled to a microfluidic platform. The second level describ...

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Real time PCR based on Fluorescent Quenching of Mercaptoacetic Acid-Modified CdTe Quantum Dots for Ultrasensitive Specific Detection of Nucleic Acids

Cited by 13 publications

References 36 publications

CdTe quantum dots accelerate the speed of Pfu-based polymerase chain reaction

CdTe quantum dots accelerate the speed of Pfu-based polymerase chain reaction

Time-Resolved Fluorescence Immunoassay of Mouse IgG using Europium(Ш) Chelate-doped Silica Nanoparticles

Biological Application of Digital Microfluidics Technology

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