CdTe quantum dots accelerate the speed of Pfu-based polymerase chain reaction

Sang, Fuming; Yang, Yang; Hexiang, Zhao; Ma, Minmin; Zhang, Zhizhou

doi:10.1080/17458080.2013.843208

Cited by 5 publications

(2 citation statements)

References 20 publications

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“…In 2009, Wang et al [6] first found that CdTe QDs could increase the specificity of the PCR at different annealing temperatures with DNA templates of different lengths. Also, CdTe QDs were reported to accelerate PCR speed [50]. Then a Pfu polymerase based multi-round PCR technique was developed through being assisted by CdTe QDs, and the specificity could be retained even in ninth-round amplification [51].…”

Section: Qdsmentioning

confidence: 99%

Application of Nanomaterials to Enhance Polymerase Chain Reaction

et al. 2022

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Polymerase Chain Reaction (PCR) is one of the most common technologies used to produce millions of copies of targeted nucleic acid in vitro and has become an indispensable technique in molecular biology. However, it suffers from low efficiency and specificity problems, false positive results, and so on. Although many conditions can be optimized to increase PCR yield, such as the magnesium ion concentration, the DNA polymerases, the number of cycles, and so on, they are not all-purpose and the optimization can be case dependent. Nano-sized materials offer a possible solution to improve both the quality and productivity of PCR. In the last two decades, nanoparticles (NPs) have attracted significant attention and gradually penetrated the field of life sciences because of their unique chemical and physical properties, such as their large surface area and small size effect, which have greatly promoted developments in life science and technology. Additionally, PCR technology assisted by NPs (NanoPCR) such as gold NPs (Au NPs), quantum dots (QDs), and carbon nanotubes (CNTs), etc., have been developed to significantly improve the specificity, efficiency, and sensitivity of PCR and to accelerate the PCR reaction process. This review discusses the roles of different types of NPs used to enhance PCR and summarizes their possible mechanisms.

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Section: Qdsmentioning

confidence: 99%

Application of Nanomaterials to Enhance Polymerase Chain Reaction

et al. 2022

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“…The temporal stability of photoluminescence is another crucial parameter describing a luminophore along with the quantum yield. For instance, the typical duration of a complete analytical procedure such as polymerase chain reaction 21 or tumor marker tests 22 varies from several hours up to 10-15 days. In this regard, an ageing of the NCs-containing particles in the course of time was investigated.…”

Section: Temporal Pl Properties Of Ncs-containing Particlesmentioning

confidence: 99%