Real-time monitoring of biofilm formation using a noninvasive impedance-based method

Kumar, Sriram; Nguyễn, Anh Tuấn; Goswami, Subir; Ferracane, Jack L.; Koley, Dipankar

doi:10.1016/j.snb.2022.133034

Cited by 13 publications

(5 citation statements)

References 39 publications

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“…The biofilm content cannot be measured straightforwardly, and there is a considerable shortage of harmony among the variety of methods adopted to study and grow biofilms [61,62]. The formation of biofilm was not investigated in the experiment.…”

Section: Biofilm Evolutionmentioning

confidence: 99%

“…The comparison of the productive rates of CaCO 3 and the suspended biomass concentrations between the experimental and calculated outcomes indirectly confirmed the accuracy of the biofilm model and the existence of a biofilm. The biofilm content cannot be measured straightforwardly, and there is a considerable shortage of harmony among the variety of methods adopted to study and grow biofilms [61,62]. The formation of biofilm was not investigated in the experiment.…”

Section: Biofilm Evolutionmentioning

confidence: 99%

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A Novel Mathematical Model for Repairing Rough Cracks Using the Microbially Induced Carbonate Precipitation (MICP)

Zhang,

Wang,

Ahmed

et al. 2023

Sustainability

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Concrete cracks have an adverse effect on the strength properties and durability of concrete structures. Therefore, repairing concrete cracks to recover the concrete’s strength parameters is an important task in the civil engineering field. For repairing concrete cracks, the MICP technique has been widely analyzed in recent times; however, no research has been conducted to deeply investigate the repair effects of MICP on concrete cracks with a rough surface using a theoretical model. In the current research, MICP with a novel mathematical model was conducted considering the precipitation of calcium carbonate (CaCO3), ureolysis, suspended biomass, geochemistry, transport of solutes, and biofilm growth. Furthermore, crack repair experiments were performed to assess the performance of the new mathematical model. The results revealed that the calculated concentrations of suspended biomass in cracks gradually decreased during the test. The comparison between the experimental results and calculated results verified the precision of the migration behavior of the suspended biomass. At the inlet, the solute concentrations and volume fractions of biofilm were higher, causing an increase in the productive rates of calcium carbonate. The consumed concentrations of solutes were higher for cracks with a smoother surface, eventually leading to smaller values of sonic time; the upper parts of the cracks also had smaller values of sonic time, showing good repair effects. The proposed mathematical model provides a better solution to control the repair time and microbial metabolism process, allowing for adjustive bioremediation and biomineralization of concrete, which could provide a firm basis for the remediation of materials in the civil engineering field.

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Section: Biofilm Evolutionmentioning

confidence: 99%

Section: Biofilm Evolutionmentioning

confidence: 99%

A Novel Mathematical Model for Repairing Rough Cracks Using the Microbially Induced Carbonate Precipitation (MICP)

Zhang,

Wang,

Ahmed

et al. 2023

Sustainability

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“…Many different methods are used to test for new antibiofilm agents. These are classified as direct and indirect methods such as microscopy (light microscopy, FISH, confocal microscopy, TEM, SEM, AFM), the roll plate method, mass spectrometry, QCM-based method, impedance-based method, Raman spectroscopy, Congo Red agar method, crystal violet method in plates/tubes, PCR, and nuclease-based method (Kırmusaoğlu 2019 ; Mozioğlu and Kocagöz 2020 ; Goudarzi et al 2021 ; Slade et al 2022 ; Kamimura et al 2022 ; Salazar et al 2023 ; Kumar et al 2023 ). Some of them (such as PCR, SEM, TEM, mass spectrometry, AFM, Raman, QCM and impedance-based methods) require sophisticated expensive instrumentation and are not suitable for high throughput studies, especially for the study of multiple antibiofilm agents at different concentrations, while others (crystal violet and Congo Red agar methods) except nuclease-based method (Mozioğlu and Kocagöz 2020 ) are easy to perform but semi-quantitative methods (Slobbe et al 2009 ).…”

Section: Introductionmentioning

confidence: 99%

New-generation biofilm effective antimicrobial peptides and a real-time anti-biofilm activity assay: CoMIC

Polat,

Soyhan,

Cebeci

et al. 2024

Appl Microbiol Biotechnol

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Nowadays, it is very important to produce new-generation drugs with antimicrobial properties that will target biofilm-induced infections. The first target for combating these microorganisms, which are the source itself. Antimicrobial peptides, which are more effective than antibiotics due to their ability to kill microorganisms and use a different metabolic pathway, are among the new options today. The aim of this study is to develop new-generation antibiotics that inhibit both biofilm-producing bacteria and the biofilm itself. For this purpose, we designed four different peptides by combining two amino acid forms (D- and L-) with the same sequence having alpha helix structures. It was found that the combined use of these two forms can increase antimicrobial efficacy more than 30-fold. These results are supported by molecular modeling and scanning electron microscopy (SEM), at the same time cytotoxicity (IC50) and hemotoxicity (HC50) values remained within the safe range. Furthermore, antibiofilm activities of these peptides were investigated. Since the existing biofilm inhibition methods in the literature do not technically simulate the exact situation, in this study, we have developed a real-time observable biofilm model and a new detection method based on it, which we call the CoMIC method. Findings have shown that the NET1 peptide with D-leucine amino acid in its structure and the NET3 peptide with D-arginine amino acid in its structure are effective in inhibiting biofilm. As a conclusion, our peptides can be considered as potential next-generation broad-spectrum antibiotic molecule/drug candidates that might be used in biofilm and clinical important bacteria. Key points • Antimicrobial peptides were developed to inhibit both biofilms producing bacteria and the biofilm itself. • CoMIC will fill a very crucial gap in understanding biofilms and conducting the necessary quantitative studies. • Molecular modelling studies, NET1 peptide molecules tends to move towards and adhere to the membrane within nanoseconds.

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“…Numerous biosensors have been developed based on the AC impedance technique. , Wang et al used phage as a novel molecular probe to achieve rapid and specific detection of Salmonella in food matrices by electrochemical impedance spectroscopy (EIS). Koley et al . described a noninvasive electrochemical impedance method for real-time monitoring of the growth of multiple plaque-derived biofilms on a range of substrates.…”

Section: Introductionmentioning

confidence: 99%

Smart Implant with Bacteria Monitoring and Killing Ability for Orthopedic Applications

Liang

et al. 2023

ACS Appl. Mater. Interfaces

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Bacterial infections around implants constitute a significant cause of implant failures. Early recognition of bacterial adhesion is an essential factor in preventing implant infections. Therefore, an implant capable of detecting and disinfecting initial bacterial adhesion is required. This study reports on the development of an intelligent solution for this issue. We developed an implant integrated with a biosensor electrode based on alternating current (AC) impedance technology to monitor the early growth process of Escherichia coli (E. coli) and its elimination. The biosensor electrode was fabricated by coating polypyrrole (PPy) doped with sodium p-toluenesulfonate (TSONa) on titanium (Ti) surfaces. Monitoring the change in resistance using electrochemical impedance spectroscopy (EIS), combined with an equivalent circuit model (ECM), enables the monitoring of the early adhesion of E. coli. The correlation with the classical optical density (OD) monitoring value reached 0.989. Subsequently, the eradication of bacteria on the electrode surface was achieved by applying different voltages to E. coli cultured on the electrode surface, which caused damage to E. coli. Furthermore, in vitro cellular experiments showed that the PPy coating has good biocompatibility and can promote bone differentiation.

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Real-time monitoring of biofilm formation using a noninvasive impedance-based method

Cited by 13 publications

References 39 publications

A Novel Mathematical Model for Repairing Rough Cracks Using the Microbially Induced Carbonate Precipitation (MICP)

A Novel Mathematical Model for Repairing Rough Cracks Using the Microbially Induced Carbonate Precipitation (MICP)

New-generation biofilm effective antimicrobial peptides and a real-time anti-biofilm activity assay: CoMIC

Smart Implant with Bacteria Monitoring and Killing Ability for Orthopedic Applications

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